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Stimulatory effect of growth in the presence of alcohols on biotransformation of penicillin G into cephalosporin-type antibiotics by resting cells of Streptomyces clavuligerus NP1

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Abstract

Growth of Streptomyces clavuligerus NP1 in the presence of methanol or ethanol resulted in a marked increase in production of cephalosporin(s) from penicillin G by resting cells. The mycelium produced in alcohol-supplemented medium was fragmented and dispersed as compared with growth in control medium. HPLC analysis showed that at least two products were present in the biotransformation supernatant fluid after 1 h incubation. One of them has been identified as deacetoxycephalosporin G (DAOG).

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Authors and Affiliations

  1. Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA e-mail: demain@mit.edu Tel.: +1-617-2531711 Fax: +1-617-2538699, , , , , , US

    M.-J. Fernández, J. L. Adrio & A. L. Demain

  2. Department of Biology, Northeastern University, Boston, MA 02115, USA, , , , , , US

    J. M. Piret

  3. Department of Chemistry, Simon Fraser University, Burnaby, BC, Canada, V5A1S6, , , , , , CA

    S. Wolfe & S. Ro

Authors
  1. M.-J. Fernández
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  2. J. L. Adrio
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  3. J. M. Piret
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  4. S. Wolfe
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  5. S. Ro
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  6. A. L. Demain
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Received: 9 December 1998 / Received revision: 29 March 1999 / Accepted: 16 April 1999

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Fernández, MJ., Adrio, J., Piret, J. et al. Stimulatory effect of growth in the presence of alcohols on biotransformation of penicillin G into cephalosporin-type antibiotics by resting cells of Streptomyces clavuligerus NP1. Appl Microbiol Biotechnol 52, 484–488 (1999). https://doi.org/10.1007/s002530051549

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  • DOI: https://doi.org/10.1007/s002530051549

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