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Definition of the Region of Interest for the Assessment of Alveolar Bone Repair Using Micro-computed Tomography

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Abstract

The objective of this study was to evaluate the influence of the extraction socket (distal or lingual root) and the type of region of interest (ROI) definition (manual or predefined) on the assessment of alveolar repair following tooth extraction using micro-computed tomography (micro-CT). The software package used for scanning, reconstruction, reorientation, and analysis of images (NRecon®, DataViewer®, CT-Analyzer®) was acquired through Bruker < https://www.bruker.com > . The sample comprised the micro-CT volumes of seven Wistar rat mandibles, in which the right first molar was extracted. The reconstructed images were analyzed using the extraction sockets, i.e., the distal and intermediate lingual root and the method of ROI definition: manual (MA), central round (CR), and peripheral round (PR). The bone volume fraction (BV/TV) values obtained were analyzed by two-way ANOVA with Tukey’s post hoc test (α = 5%). The distal extraction socket resulted in significantly lower BV/TV values than the intermediate lingual socket for MA (P = 0.001), CR (P < 0.001), and PR (P < 0.001). Regarding the ROI, when evaluating the distal extraction socket, the BV/TV was significantly higher (P < 0.001) for MA than for CR and PR, with a lower BV/TV for CR. However, no significant difference was observed for MA (P = 0.855), CR (P = 0.769), or PR (P = 0.453) in the intermediate lingual extraction socket. The bone neoformation outcome (BV/TV) for alveolar bone repair after tooth extraction is significantly influenced by the ROI and the extraction socket. Using the predefined method with a standardized ROI in the central region of the distal extraction socket resulted in the assessment of bone volume, demonstrating the most critical region of the bone neoformation process.

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Acknowledgements

The authors are grateful to Rede de Biotérios de Roedores da Universidade Federal de Uberlândia, Centro de Pesquisa Odontológico-Biomecânica, Biomateriais e Biologia celular (CPBIO).

Funding

This study was supported by grants from the Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG), the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior–Brasil (CAPES)–Finance Code 001 and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). The authors deny any conflicts of interest related to this study.

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Authors and Affiliations

  1. Department of Periodontology and Implantology, School of Dentistry, Federal University of Uberlândia, Avenida Pará s/n°, Campus Umuarama, Bloco 4L, Bairro Umuarama, Uberlândia, Minas Gerais, 38400-902, Brazil

    Juliana Simeão Borges, Vitor Cardoso Costa, Milena Suemi Irie & Priscilla Barbosa Ferreira Soares

  2. Department of Oral Diagnosis, School of Dentistry, Federal University of Uberlândia, Uberlândia, Minas Gerais, Brazil

    Gabriella Lopes de Rezende Barbosa

  3. Department of Dentistry and Oral Health, Section for Oral Radiology, Health, Aarhus University, Aarhus, Denmark

    Rubens Spin-Neto

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Contributions

PBFS, RSN, and JSB participated in the conception and study design. JSB and VCC performed the experiment. MSI and JSB contributed to the interpretation of the experimental results and statistical analysis. JSB, VCC, and MSI wrote the manuscript. All authors discussed the results and commented on the manuscript.

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Correspondence to Priscilla Barbosa Ferreira Soares.

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This study was approved by the Bioethics Committee for Animal Experimentation (#013/19) at Federal University of Uberlândia, Brazil, and conducted in accordance with the ARRIVE guidelines for preclinical studies and the normative guidelines of the National Council for Animal Control and Experimentation (CONCEA).

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Borges, J.S., Costa, V.C., Irie, M.S. et al. Definition of the Region of Interest for the Assessment of Alveolar Bone Repair Using Micro-computed Tomography. J Digit Imaging 36, 356–364 (2023). https://doi.org/10.1007/s10278-022-00693-w

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