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Rapid detection for sporeless trait from Pleurotus pulmonarius culture extracts by using real-time PCR

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Mycoscience

Abstract

The release and spread of a large number of basidiospores from the developing fruiting body cause serious problems in the cultivation of edible mushrooms, including Pleurotus pulmonarius (Fr.) Quel. The P. pulmonarius sporeless mutant, TMIC-30058, has a high potential for breeding sporeless cultivars to reduce these adverse effects. Our previous study (Okuda et al. in Breeding Science 59:315–319, 2009) showed two sequence-tagged site (STS) markers for the sporeless trait from this mutant. Here, using these STS markers we present rapid detection of sporeless trait from P. pulmonarius culture extracts by real-time PCR. This method enables us to cut down time and labor for screening of the sporeless trait, suggesting its availability for efficient breeding of sporeless cultivars.

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Acknowledgments

This research was supported in part by the Japan Society for the Promotion of Science (Grants-in-Aid for Scientific Research, KibanC 19580004).

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Authors and Affiliations

  1. Faculty of Agriculture, Tottori University, 4-101 Koyamacho-Minami, Tottori, 680-8553, Japan

    Yasuhito Okuda & Teruyuki Matsumoto

  2. Analytical and Measuring Instruments Division, Shimadzu Corporation, 1 Nishinokyo-Kuwabaracho, Nakagyoku, Kyoto, 604-8511, Japan

    Kenji Ninomiya

Authors
  1. Yasuhito Okuda
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  2. Teruyuki Matsumoto
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  3. Kenji Ninomiya
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Corresponding author

Correspondence to Yasuhito Okuda.

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Okuda, Y., Matsumoto, T. & Ninomiya, K. Rapid detection for sporeless trait from Pleurotus pulmonarius culture extracts by using real-time PCR. Mycoscience 52, 143–146 (2011). https://doi.org/10.1007/s10267-010-0078-z

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  • DOI: https://doi.org/10.1007/s10267-010-0078-z

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