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Agrobacterium tumefaciens-mediated genetic transformation of the white root rot ascomycete Rosellinia necatrix

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Mycoscience

Abstract

Hygromycin B resistance was conferred to the mycelium of the white root rot fungus Rosellinia necatrix by transformation with the hygromycin B phosphotransferase gene (hph) of Escherichia coli under the control of the heterologous fungal Aspergillus nidulans P-gpd (glyceraldehyde 3-phosphate dehydrogenase) promoter and the trpC terminator. In all three transformants, the presence of hph and single-copy integrations of the marker gene were demonstrated by Southern analysis. This is the first report describing A. tumefaciens-mediated transformation of R. necatrix

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Authors and Affiliations

  1. Faculty of Agriculture, Tottori University, Tottori, Japan

    Tadanori Aimi & Yutaka Kitamoto

  2. Department of Bioresource Development, Hiroshima Prefectural University, Nanatsukahara, Shobara, Hiroshima, 727-0023, Japan

    Hiroyuki Taguchi, Yoshikazu Tanaka & Tsutomu Morinaga

Authors
  1. Tadanori Aimi
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  2. Hiroyuki Taguchi
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  3. Yoshikazu Tanaka
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  4. Yutaka Kitamoto
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  5. Tsutomu Morinaga
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Corresponding author

Correspondence to Tsutomu Morinaga.

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Aimi, T., Taguchi, H., Tanaka, Y. et al. Agrobacterium tumefaciens-mediated genetic transformation of the white root rot ascomycete Rosellinia necatrix . Mycoscience 46, 27–31 (2005). https://doi.org/10.1007/s10267-004-0210-z

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  • DOI: https://doi.org/10.1007/s10267-004-0210-z

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