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Effects of rice fermented extracts, “Sake Lees”, on the functional activity of odontoblast-like cells (KN-3 cells)

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Abstract

This study was designed to investigate the effects of Sake Lees extracts (SLE, Sake Kasu) on the functional activity of odontoblastic cells and tooth pulp of the rats. For in vitro studies, a rat clonal odontoblast-like cell line, KN-3 cells were cultured. SLE significantly decreased KN-3 cell proliferation, but showed no significant cytotoxicity. SLE effects on several protein productions of KN-3 cells were compared with PBS. SLE and PBS increased alkaline phosphatase (ALP), dentin sialoprotein (DSP), and osterix in a day-course dependent manner, while SLE increased the induction of ALP on day 9–21 and DSP on day 15–21. SLE also increased Runx2 expression on day 3 and 9 compared to PBS. Alizarin Red stainings revealed that SLE showed a subtle increase in mineralization of KN-3 cells on day 15 and 21. A histological investigation was conducted to assess if SLE induced reparative dentin formation after direct capping at the exposed tooth pulp in rats, suggesting that SLE could increase the reparative dentin formation more than PBS. These findings suggest that Sake Lees could have functional roles in the alterations of odontoblastic activity, which might influence the physiology of the tooth pulp.

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Acknowledgements

This work was supported by the Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research (KAKENHI; Grant Number, 19K10353).

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Authors and Affiliations

  1. Division of Oral Physiology, Niigata University Graduate School of Medical and Dental Sciences, 2-5274, Gakkocho-dori, Chuo-ku, Niigata, 951-8514, Japan

    Keiichiro Okamoto & Kensuke Yamamura

  2. Division of Dental Pharmacology, Niigata University Graduate School of Medical and Dental Sciences, 2-5274, Gakkocho-dori, Chuo-ku, Niigata, 951-8514, Japan

    Yoshito Kakihara & Makio Saeki

  3. Division of Cariology, Operative Dentistry and Endodontics, Niigata University Graduate School of Medical and Dental Sciences, 2-5274, Gakkocho-dori, Chuo-ku, Niigata, 951-8514, Japan

    Naoto Ohkura, Aiko Tohma & Yuichiro Noiri

  4. Division of Endodontics and Restorative Dentistry, Department of Oral Functions, Kyushu Dental University, 2-6-1, Manazuru, Kokura-kita, Kitakyushu, Fukuoka, 803-8580, Japan

    Ayako Washio & Chiaki Kitamura

  5. Sakeology Center, Niigata University, Niigata, Japan

    Keiichiro Okamoto & Yoshito Kakihara

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  1. Keiichiro Okamoto
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  2. Yoshito Kakihara
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  3. Naoto Ohkura
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  4. Aiko Tohma
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  6. Chiaki Kitamura
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  7. Yuichiro Noiri
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  8. Kensuke Yamamura
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  9. Makio Saeki
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Correspondence to Keiichiro Okamoto.

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Okamoto, K., Kakihara, Y., Ohkura, N. et al. Effects of rice fermented extracts, “Sake Lees”, on the functional activity of odontoblast-like cells (KN-3 cells). Odontology 110, 254–261 (2022). https://doi.org/10.1007/s10266-021-00654-9

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  • DOI: https://doi.org/10.1007/s10266-021-00654-9

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