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Three-dimensional observation of the mouse embryo by micro-computed tomography: Meckel’s cartilage, otocyst, and/or muscle of tongue

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Abstract

Three-dimensional observation during embryogenesis is possible with micro-computed tomography, but there are no observations of organ size. In this paper, three examples of three-dimensional observation of organs by micro-CT are tried. At 13.0 days post-coitum, mouse embryos were fixed in 4% paraformaldehyde for 24 h and stained enbloc by osmium tetroxide overnight. The embryos were then embedded in paraffin using standard methods for 24 h. Specimens were analyzed by micro-computed tomography and image processing was performed. The entire Meckel’s cartilage and its relation in the mandible, as well as the complex structure of the otocyst, are easily visualized. Although it is difficult to extract detailed structures of the tongue muscles, it is possible to identify the inner and external tongue muscles. Relation among the organs and other are easily visualized. Three-dimensional observation by micro-computed tomography is an important technology for visualization of embryogenesis and could be used in organ culture.

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Acknowledgments

We are grateful to Prof. T. Kimura of the Department of Oral Biochemistry at the Nippon Dental University School of Life Dentistry at Niigata for his encouragement and constant support. We also wish to thank Prof. A. Ezura of General Dentistry at the Nippon Dental University School of Life Dentistry at Niigata for his help and suggestions. This research was supported by Research Promotion Grants (No. NDUF-10-07) from the Nippon Dental University.

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Authors and Affiliations

  1. Advanced Research Center, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan

    Hidekazu Aoyagi & Shin-ichi Iwasaki

  2. Department of Biology, Matsumoto Dental University School of Dentistry, 1780 Hirookagouhara, Shiojiri, Nagano, 399-0781, Japan

    Hideki Yoshizawa

  3. Department of Oral and Maxillofacial Surgery, The Nippon Dental University School of Life Dentistry at Niigata, Niigata, Japan

    Kohzo Tsuchikawa

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  1. Hidekazu Aoyagi
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  2. Shin-ichi Iwasaki
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  3. Hideki Yoshizawa
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  4. Kohzo Tsuchikawa
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Correspondence to Hidekazu Aoyagi.

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Aoyagi, H., Iwasaki, Si., Yoshizawa, H. et al. Three-dimensional observation of the mouse embryo by micro-computed tomography: Meckel’s cartilage, otocyst, and/or muscle of tongue. Odontology 100, 137–143 (2012). https://doi.org/10.1007/s10266-011-0041-2

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  • DOI: https://doi.org/10.1007/s10266-011-0041-2

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