Abstract
The purpose of this study was to demonstrate a micro-computed tomography (CT) method for observations of the mouse embryo. At 13.0 days post-coitum, mouse embryos were fixed in 4% paraformaldehyde for 24 h and stained en bloc by osmium tetroxide overnight. The embryos were then embedded in paraffin using standard methods for 24 h. Specimens were analyzed by micro-CT and image processing was performed. Organs containing nervous and blood systems could be viewed as a result of different osmium-staining densities. The trigeminal ganglion was imaged using three-dimensional techniques. Observation of the embryo was possible by micro-CT with osmium tetroxide staining.
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Aoyagi, H., Tsuchikawa, K. & Iwasaki, Si. Three-dimensional observation of the mouse embryo by micro-computed tomography: composition of the trigeminal ganglion. Odontology 98, 26–30 (2010). https://doi.org/10.1007/s10266-009-0112-9
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DOI: https://doi.org/10.1007/s10266-009-0112-9