Journal of Plant Research

, Volume 130, Issue 5, pp 909–927 | Cite as

Enhanced photosynthetic capacity increases nitrogen metabolism through the coordinated regulation of carbon and nitrogen assimilation in Arabidopsis thaliana

  • Kumi Otori
  • Noriaki Tanabe
  • Toshiki Maruyama
  • Shigeru Sato
  • Shuichi Yanagisawa
  • Masahiro Tamoi
  • Shigeru Shigeoka
Regular Paper

Abstract

Plant growth and productivity depend on interactions between the metabolism of carbon and nitrogen. The sensing ability of internal carbon and nitrogen metabolites (the C/N balance) enables plants to regulate metabolism and development. In order to investigate the effects of an enhanced photosynthetic capacity on the metabolism of carbon and nitrogen in photosynthetically active tissus (source leaves), we herein generated transgenic Arabidopsis thaliana plants (ApFS) that expressed cyanobacterial fructose-1,6-/sedoheptulose-1,7-bisphosphatase in their chloroplasts. The phenotype of ApFS plants was indistinguishable from that of wild-type plants at the immature stage. However, as plants matured, the growth of ApFS plants was superior to that of wild-type plants. Starch levels were higher in ApFS plants than in wild-type plants at 2 and 5 weeks. Sucrose levels were also higher in ApFS plants than in wild-type plants, but only at 5 weeks. On the other hand, the contents of various free amino acids were lower in ApFS plants than in wild-type plants at 2 weeks, but were similar at 5 weeks. The total C/N ratio was the same in ApFS plants and wild-type plants, whereas nitrite levels increased in parallel with elevations in nitrate reductase activity at 5 weeks in ApFS plants. These results suggest that increases in the contents of photosynthetic intermediates at the early growth stage caused a temporary imbalance in the free-C/free-N ratio and, thus, the feedback inhibition of the expression of genes involved in the Calvin cycle and induction of the expression of those involved in nitrogen metabolism due to supply deficient free amino acids for maintenance of the C/N balance in source leaves of ApFS plants.

Keywords

Biomass Calvin cycle C/N balance Fructose-1,6-bisphosphatase Photosynthesis Sedoheptulose-1,7-bisphosphatase 

Supplementary material

10265_2017_950_MOESM1_ESM.pdf (746 kb)
Supplementary material 1 (PDF 745 KB)

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Copyright information

© The Botanical Society of Japan and Springer Japan 2017

Authors and Affiliations

  • Kumi Otori
    • 1
    • 2
  • Noriaki Tanabe
    • 1
    • 2
  • Toshiki Maruyama
    • 1
  • Shigeru Sato
    • 3
  • Shuichi Yanagisawa
    • 2
    • 3
  • Masahiro Tamoi
    • 1
    • 2
  • Shigeru Shigeoka
    • 1
    • 2
  1. 1.Department of Advanced Bioscience, Faculty of AgricultureKindai UniversityNaraJapan
  2. 2.Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology AgencyKawaguchiJapan
  3. 3.Biotechnology Research CenterThe University of TokyoBunkyo-kuJapan

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