Abstract
The importance of the phosphorylated pathway (PPSB) of l-serine (Ser) biosynthesis in plant growth and development has been demonstrated, but its specific role in leaves and interaction with photorespiration, the main leaf Ser biosynthetic pathway at daytime, are still unclear. To investigate whether changes in biosynthesis of Ser by the PPSB in leaves could have an impact on photorespiration and plant growth, we overexpressed PSP1, the last enzyme of this pathway, under control of the Cauliflower Mosaic Virus 35S promoter in Arabidopsis thaliana. Overexpressor plants grown in normal air displayed larger rosette diameter and leaf area as well as higher fresh and dry weight than the wild type. By contrast, no statistically significant differences to the wild type were observed when the overexpressor seedlings were transferred to elevated CO2, indicating a relationship between PSP1 overexpression and photorespiration. Additionally, the transgenic plants displayed higher photorespiration, an increase in CO2 net-uptake and stronger expression in the light of genes encoding enzymes involved in photorespiration. We further demonstrated that expression of many genes involved in nitrogen assimilation was also promoted in leaves of transgenic plants and that leaf nitrate reductase activity increased in the light, too, although not in the dark. Our results suggest a close correlation between the function of PPSB and photorespiration, and also nitrogen metabolism in leaves.
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Acknowledgements
We wish to thank Prof. Hermann Bauwe and Dr. Stefan Timm for their valuable advices during our collaboration and critical reading of our manuscript. The first author also wishes to thank Dr. Ramona Kern for her help during the author’s visit in Prof. Bauwe’s lab.This work was supported by the National Natural Science Foundation of China (Grant No. 31270296), a PPP Project of CSC-DAAD, and 111 Project B08011.
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XH, YZ, YB, and YW designed the research. XH, KP, HW, and SS performed the research. XH and YW analyzed the data. XH and YW wrote the article, which was later approved by all the other authors.
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Han, X., Peng, K., Wu, H. et al. Overexpression of PSP1 enhances growth of transgenic Arabidopsis plants under ambient air conditions. Plant Mol Biol 94, 419–431 (2017). https://doi.org/10.1007/s11103-017-0615-7
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DOI: https://doi.org/10.1007/s11103-017-0615-7