Journal of Plant Research

, Volume 130, Issue 3, pp 559–570 | Cite as

Biochemical response of hybrid black poplar tissue culture (Populus × canadensis) on water stress

  • B. M. Popović
  • D. Štajner
  • R. Ždero-Pavlović
  • I. Tari
  • J. Csiszár
  • Á. Gallé
  • P. Poór
  • V. Galović
  • B. Trudić
  • S. Orlović
Regular Paper

Abstract

In this study, poplar tissue culture (hybrid black poplar, M1 genotype) was subjected to water stress influenced by polyethyleneglycol 6000 (100 and 200 mOsm PEG 6000). The aim of the research was to investigate the biochemical response of poplar tissue culture on water deficit regime. Antioxidant status was analyzed including antioxidant enzymes, superoxide-dismutase (SOD), catalase (CAT), guiacol-peroxidase (GPx), glutathione-peroxidase (GSH-Px), glutathione-reductase, reduced glutathione, total phenol content, Ferric reducing antioxidant power and DPPH radical antioxidant power. Polyphenol oxidase and phenylalanine-ammonium-lyase were determined as enzymatic markers of polyphenol metabolism. Among oxidative stress parameters lipid peroxidation, carbonyl-proteins, hydrogen-peroxide, reactive oxygen species, nitric-oxide and peroxynitrite were determined. Proline, proline-dehydrogenase and glycinebetaine were measured also as parameters of water stress. Cell viability is finally determined as a biological indicator of osmotic stress. It was found that water stress induced reactive oxygen and nitrogen species and lipid peroxidation in leaves of hybrid black poplar and reduced cell viability. Antioxidant enzymes including SOD, GPx, CAT and GSH-Px were induced but total phenol content and antioxidant capacity were reduced by PEG 6000 mediated osmotic stress. The highest biochemical response and adaptive reaction was the increase of proline and GB especially by 200 mOsm PEG. While long term molecular analysis will be necessary to fully address the poplar potentials for water stress adaptation, our results on hybrid black poplar suggest that glycine-betaine, proline and PDH enzyme might be the most important markers of poplar on water stress and that future efforts should be focused on these markers and strategies to enhance their concentration in poplar.

Keywords

Poplar Tissue culture Osmotic stress Antioxidant Proline Glycinebetaine 

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Copyright information

© The Botanical Society of Japan and Springer Japan 2017

Authors and Affiliations

  • B. M. Popović
    • 1
  • D. Štajner
    • 1
  • R. Ždero-Pavlović
    • 1
  • I. Tari
    • 2
  • J. Csiszár
    • 2
  • Á. Gallé
    • 2
  • P. Poór
    • 2
  • V. Galović
    • 3
  • B. Trudić
    • 3
  • S. Orlović
    • 3
  1. 1.Department of Field and Vegetable Crops, Faculty of AgricultureUniversity of Novi SadNovi SadSerbia
  2. 2.Department of Plant BiologyUniversity of SzegedSzegedHungary
  3. 3.Institute of Lowland Forestry and EnvironmentNovi SadSerbia

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