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NtbZIP60, an endoplasmic reticulum-localized transcription factor, plays a role in the defense response against bacterial pathogens in Nicotiana tabacum

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Abstract

A spermine-based signal transduction pathway plays a defensive role against incompatible pathogens. We identified a novel spermine-responsive cDNA from Nicotiana tabacum that encodes a basic region/leucine zipper protein with a putative transmembrane domain. Identity to Arabidopsis thaliana AtbZIP60 was sufficiently high to name the novel cDNA NtbZIP60. Expression analysis revealed that NtbZIP60 is a component of the spermine-signal pathway, and is also involved in the unfolded protein response (UPR), as demonstrated for AtbZIP60. The gene product, NtbZIP60, localizes to the endoplasmic reticulum (ER) in plant cells; once the putative transmembrane domain is eliminated from the intact protein, it targets the nucleus. The putative processed form of NtbZIP60 transactivates target genes through binding to plant-specific UPR cis-elements. Expression of NbbZIP60, an NtbZIP60 ortholog in Nicotiana benthamiana, was significantly up-regulated at 6 h and later time points upon infection with the non-host pathogen Pseudomonas cichorii, while it was unaffected by infection with the compatible pathogen Pseudomonas syringae pv. tabaci. Furthermore, NbbZIP60-silenced N. benthamiana plants allowed higher multiplication of P. cichorii compared to the control plants. Taken together, the results suggest that this ER-localized transcription factor is involved in the spermine-signal transduction pathway and plays an important role in plant innate immunity.

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Abbreviations

AZC:

2-Azetidine carboxylic acid

BiP:

Luminal binding protein

bZIP:

Basic region/leucine zipper transcription factor

BK:

Bonkrekic acid

DMSO:

Dimethyl sulfoxide

DTT:

Dithiothreitol

ER:

Endoplasmic reticulum

ERSE:

ER stress response element

Flav:

Flavone

GFP:

Green fluorescent protein

HR:

Hypersensitive response

NAC:

N-Acetylcysteine

PR:

Pathogenesis-related

PTP:

Permeability transition pore

TMV:

Tobacco mosaic virus

TRV:

Tobacco rattle virus

UPR:

Unfolded protein response

UPRE:

UPR element

VIGS:

Virus-induced gene silencing

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Acknowledgments

We thank Drs. D.C. Baulcombe (John Innes Center, Norwich, UK), T. Okamoto (Tokyo Metropolitan University, Japan) and Y. Niwa (Shizuoka University, Japan) for providing the TRV vector system, the ER-targeting DsRed plasmid and 35S::sGFP(S65T) plasmid, respectively. We also thank Drs. Y. Hikichi (Kochi University, Japan) and T. Ichinose (Okayama University, Japan) for providing us with bacterial strains. Dr. M.R. Shenton is acknowledged for critical reading of the manuscript. This research was partly supported by Grant-in-Aids from the Japan Society for the Promotion of Science to T.K. (19658039) and to Y.T. (19780002), and grants from Saito Gratitude Foundation to C.T. and the Sumitomo Foundation to Y.T.

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Authors and Affiliations

  1. Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai, Miyagi, 980-8577, Japan

    Chika Tateda, Rei Ozaki, Yu Onodera, Yoshihiro Takahashi, Koji Yamaguchi, Thomas Berberich & Tomonobu Kusano

  2. Graduate School of Agricultural Sciences, Osaka Prefectural University, 1-1 Gakuen-cho, Naka-ku, Sakai, Osaka, 599-8531, Japan

    Nozomu Koizumi

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  1. Chika Tateda
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  2. Rei Ozaki
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  3. Yu Onodera
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  4. Yoshihiro Takahashi
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  5. Koji Yamaguchi
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  6. Thomas Berberich
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  7. Nozomu Koizumi
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  8. Tomonobu Kusano
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Correspondence to Tomonobu Kusano.

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Chika Tateda and Rei Ozaki contributed equally to this work.

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Tateda, C., Ozaki, R., Onodera, Y. et al. NtbZIP60, an endoplasmic reticulum-localized transcription factor, plays a role in the defense response against bacterial pathogens in Nicotiana tabacum . J Plant Res 121, 603–611 (2008). https://doi.org/10.1007/s10265-008-0185-5

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