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Rhizoid differentiation of Spirogyra is regulated by substratum

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Abstract

Some species of Spirogyra can anchor to substratum with rod- or rosette-shaped rhizoid (hapteron). The rhizoid differentiation can be induced by cutting algal filaments in a laboratory. Requirement of contact stimulation for rhizoid differentiation has been reported (Nagata in Plant Cell Physiol 14:531–541, 1973a). However, the control mechanism of rhizoid morphology has not been elucidated. When cut filaments were incubated on the glass surface, start of tip growth, secretion of lectin-binding material and callose synthesis were observed. In the absence of contact to the glass surface, none of above phenomena was induced. Systematic analysis showed that rosette-shaped rhizoid was formed only on the hydrophobic substratum. On the hydrophobic substratum, both Bandeiraea (Griffonia) simplicifolia lectin and jacalin strongly stained the rhizoids. On the hydrophilic substratum, however, only Bandeiraea (Griffonia) simplicifolia lectin strongly stained the rhizoids.

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Abbreviations

APW:

Artificial pond water

HEPES:

N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acide

BSL-I:

Bandeiraea (Griffonia) simplicifolia lectin

WGA:

Wheat germ agglutinin

References

  • Akashi T, Kawasaki S, Shibaoka H (1990) Stabilization of cortical microtubules by the cell wall in cultured tobacco cells. Planta 182:363–369

    Article  Google Scholar 

  • Akashi T, Shibaoka H (1991) Involvement of transemembrane proteins in the association of cortical microtubules with the plasma membrane in tobacco BY-2 cells. J Cell Sci 98:169–174

    Google Scholar 

  • Baluska F, Salaj J, Mathur J, Braun M, Jasper F, Samaj J, Chua N-H, Barlow PW, Volkmann D (2000) Root hair formation: F-actin dependent tip growth is initiated by local assembly of profiling supported F-actin meshworks accumulated within expansin enriched bulges. Dev Biol 227:618–632

    Article  PubMed  CAS  Google Scholar 

  • Belanger DK, Quatrano SR (2000) Polarity: the role of the localized secretion. Curr Opin Plant Biol 3:67–72

    Article  PubMed  CAS  Google Scholar 

  • Franke WW, Herth W, van der Woude WJ, Morré DJ (1972) Tubular and filamentous structures in pollen tubes: possible involvement as guide elements in cytoplasmic streaming and vectorial migration of secretory vesicles. Planta 105:317–341

    Article  Google Scholar 

  • Hable EW, Kropf LD (1998) Role of secretion and the cytoskelton in cell adhesion and polarity establishment in Pelvetia compressa. Zygotes 198:45–56

    CAS  Google Scholar 

  • Haley A, Russell JA, Wood N, Allan CA, Knight M, Campbell KA, Trewavas JA (1995) Effects of mechanical signaling on plant cell cytosolic calcium. Proc Natl Acad Sci USA 92:4124–4128

    Article  PubMed  CAS  Google Scholar 

  • Ikegaya H, Yamada S, Sonobe S, Shimmen T (2004) Saponin-induced release of single cells from filaments and rhizoid differentiation in Spirogyra. J Plant Res 117:443–447

    Article  PubMed  Google Scholar 

  • Inoue N, Sonobe S, Nagata Y, Shimmen T (1999) Secretion of lectin-binding material in rhizoid differentiation of Spirogyra. Plant Cell Physiol 40:973–977

    CAS  Google Scholar 

  • Inoue N, Yamada S, Shimmen T (2002) Rhizoid differentiation in Spirogyra: position sensing by terminal cells. Plant Cell Physiol 43:479–483

    Article  PubMed  CAS  Google Scholar 

  • Jackson SL, Heath IB (1990) Evidence that actin reinforces the extensible hyphal apex of the oomycete Saprolegnia ferax. Protoplasma 157:144–153

    Article  Google Scholar 

  • Jaffe JM (1973) Thigmomorphogenesis: the response of plant growth and development to mechanical stimulation. Planta 114:143–157

    Article  Google Scholar 

  • Ketelaar T, de Ruijter NCA, Emons AMC (2003) Unstable F-actin specifies the region and microtubule direction of cell expansion in Arabidopsis root hair. Plant Cell 15:285–292

    Article  PubMed  CAS  Google Scholar 

  • Knight H (2000) Calcium signaling during abiotic stress in plants. Int Rev Cytol 195:269–324

    Article  PubMed  CAS  Google Scholar 

  • Legue V, Blancaflor E, Wymer C, Perbal G, Fantin D, Gilroy S (1997) Cytoplasmic free Ca2+ in Arabidopsis root changes in response to touch but not gravity. Plant Physiol 114:789–800

    Article  PubMed  CAS  Google Scholar 

  • Mascarenhas JP, Lafountain J (1972) Protoplasmic streaming, cytochalasin B, and growth of the pollen tube. Tissue Cell 4:11–14

    Article  PubMed  CAS  Google Scholar 

  • Masuda Y, Takagi S, Nagai R (1991) Protease-sensitive anchoring of microfilament bundles provides tracks for cytoplasmic streaming in Vallisneria. Protoplasma 162:151–159

    Article  CAS  Google Scholar 

  • Nagata Y (1973a) Rhizoid differentiation in Spirogyra. I. Basic features of rhizoid formation. Plant Cell Physiol 14:531–541

    Google Scholar 

  • Nagata Y (1973b) Rhizoid differentiation in Spirogyra. II. Photoreversibility of rhizoid induction by red and far-red light. Plant Cell Physiol 14:543–554

    Google Scholar 

  • Nagata Y (1977) Light-induced adhesion of Spirogyra cells to glass. Plant Physiol 59:680–683

    PubMed  Google Scholar 

  • Nagata Y (1979) Rhizoid differentiation in Spirogyra. III. Intracellular localization of phytochrome. Plant Physiol 64:9–12

    Article  PubMed  CAS  Google Scholar 

  • Nishimura T, Yokota E, Wada T, Shimmen T, Okada K (2003) An Arabidopsis ACT2 dominant-negative mutation, which disturbs F-actin polymerization, reveals its distinctive function in root development. Plant Cell Physiol 44:1131–1140

    Article  PubMed  CAS  Google Scholar 

  • Ohiwa T (1977) Preparation and culture of Spirogyra and Zygnema protoplasts. Cell Struct Funct 2:249–255

    Article  Google Scholar 

  • Pocock K, Duckett GJ (1985) On the occurrence of branched and swollen rhizoids in british hepatics: their relationships with the substratum and associations with fungi. New Phytol 99:281–304

    Article  Google Scholar 

  • Preuss ML, Serna J, Falbel TG, Bednarek SY, Nielsen E (2004) The Arabidopsis Rab GTPase Rab4b localizes to the tips of growing root hair cells. Plant Cell 16:1589–1603

    Article  PubMed  CAS  Google Scholar 

  • Ryu J, Mizuno K, Takagi S, Nagai R (1997) Extracellular components implicated in the stationary organization of the actin cytoskeleton in mesophyll cells of Vallisneria. Plant Cell Physiol 38:420–432

    PubMed  CAS  Google Scholar 

  • Schröter K (1978) Asymmetrical jelly secretion of zygotes of Pelvetia and Fucus: an early polarization event. Planta 40:69–70

    Article  Google Scholar 

  • Shaw LS, Quatrano SR (1996) The role of targeted secretion in the establishment of cell polarity and the orientation of the division plane in Fucus zygotes. Development 122:2623–2630

    PubMed  CAS  Google Scholar 

  • Srinivasan S, Vargas MM, Roberson RW (1996) Functional, organizational, and biochemical analysis of actin in hyphal tip cells of Allomyces macrogynus. Mycologia 88:57–70

    Article  CAS  Google Scholar 

  • Yamada S, Sonobe S, Shimmen T (2003) Synthesis of a Callosic substance during rhizoid differentiation in Spirogyra. Plant Cell Physiol 44:1225–1228

    Article  PubMed  CAS  Google Scholar 

  • Yoshida K, Inoue N, Sonobe S, Shimmen T (2003) Involvement of microtubules in rhizoid differentiation of Spirogyra species. Protoplasma 221:227–235

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Graduate School of Life Science, University of Hyogo, Harima Science Park City, Hyogo, 678-1297, Japan

    Hisato Ikegaya, Seiji Sonobe & Teruo Shimmen

  2. Shinko Seiki Co. Ltd, Takatsukadai, Nishi-Ku, Kobe, Hyogo, 651-2271, Japan

    Kohei Murakami

Authors
  1. Hisato Ikegaya
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  2. Seiji Sonobe
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  3. Kohei Murakami
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  4. Teruo Shimmen
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Correspondence to Teruo Shimmen.

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Ikegaya, H., Sonobe, S., Murakami, K. et al. Rhizoid differentiation of Spirogyra is regulated by substratum. J Plant Res 121, 571–579 (2008). https://doi.org/10.1007/s10265-008-0182-8

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  • DOI: https://doi.org/10.1007/s10265-008-0182-8

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