Abstract.
Oxidative injury to monocytes/macrophages is considered one of the key factors in atherogenesis. Macrophage colony stimulating factor (M-CSF) also plays an important role in the stages of atherosclerosis. Some researchers showed that M-CSF accelerated pathological changes in the early stages of atherosclerosis. However, other reports suggested that exogenous M-CSF could prevent the progress of atherosclerosis. To further investigate the role of M-CSF in atherogenesis and to elucidate the effect of M-CSF on the oxidative injury to monocytes/macrophages, RAW264.7 cell lines overexpressing M-CSF were established by applying the lipofectin transfection method. The oxidative injurious effect of tert-butylhydroperoxide on the established cell lines was investigated. Two M-CSF-transfected RAW264.7 cell lines secreted large amounts of M-CSF. Compared with the non-transfected RAW264.7 cells, M-CSF-overexpressing RAW264.7 cells were more vulnerable to oxidative injury. We conclude that M-CSF could aggravate the oxidative injury due to macrophages in some situations.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 21 August 2002 / Accepted: 12 March 2003
Correspondence to Z.-J. Pang
Rights and permissions
About this article
Cite this article
Pang, ZJ. Effect of macrophage colony stimulating factor overexpression on oxidative injury/resistance of RAW264.7 cells. Clin Exp Med 3, 20–26 (2003). https://doi.org/10.1007/s102380300011
Issue Date:
DOI: https://doi.org/10.1007/s102380300011