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Rediscovery of Oryzias hubbsi with notes on its reproductive isolation with O. javanicus

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Abstract

Oryzias hubbsi Roberts 1998 is a miniature species of adrianichthyid (medaka). It was described based on a laboratory stock bred from individuals collected in the field in 1983. No individuals of this species have been collected in the field since then. Mitochondrial haplotyping revealed that individuals collected from a fishing pond at Tangerang, west of Jakarta, were Oryzias javanicus (Bleeker 1854), a closely related common species widely distributed throughout the Sunda Islands, as well as O. hubbsi. Phylogenetic network and population genetic structure analyses on the basis of genome-wide single-nucleotide polymorphisms revealed that O. hubbsi and O. javanicus individuals are clearly separated from each other, indicating that they do not hybridize. Morphometric and meristic characters of the newly discovered O. hubbsi individuals were consistent with those of previous studies. We discussed the reasons for the lack of reports of O. hubbsi since the 1980s and the mechanisms of reproductive isolation between O. hubbsi and O. javanicus.

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Acknowledgements

We thank the Ministry of Research, Technology, and Higher Education, Republic of Indonesia (RISTEKDIKTI) and National Research and Innovation Agency (BRIN), Indonesia, and Centre for Marine and Coastal Studies (CEMACS), Universiti Sains Malaysia (USM), Malaysia, for providing research permits. We thank the National Bio-Resource Project (NBRP) of the Ministry of Education, Science, Sports and Culture of Japan for providing laboratory stock individuals. We thank Erina Fukunaga and Chu-Hong Liao for technical assistance with the experiments and Chris Akcali, PhD, from Edanz (http://jp.edanz.com/ac) for editing a draft of this manuscript. This study was supported by JSPS KAKENHI (No. 19H03269 and 23H02524) to YT and (No. 23570031 and 21H04782) to KY.

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Authors and Affiliations

  1. Museum Zoologicum Bogoriense, Research Center for Biosystematics and Evolution, National Research and Innovation Agency, Cibinong, 16911, Indonesia

    Daniel F. Mokodongan & Ilham V. Utama

  2. Tropical Biosphere Research Center, University of the Ryukyus, Okinawa, 903-0213, Japan

    Ilham V. Utama & Kazunori Yamahira

  3. Faculty of Agriculture, Ryukoku University, Otsu, 520-2194, Japan

    Atsushi J. Nagano

  4. Institute for Advanced Biosciences, Keio University, Tsuruoka, 997-0017, Japan

    Atsushi J. Nagano

  5. Centre for Marine and Coastal Studies, Universiti Sains Malaysia (USM), 11800, Gelugor, Malaysia

    Sau Pinn Woo & Shau Hwai Tan

  6. Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan

    Satoshi Ansai

  7. Faculty of Bio-Science, Nagahama Institute of Bio-Science and Technology, Nagahama, 526-0829, Japan

    Yusuke Takehana

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  1. Daniel F. Mokodongan
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Correspondence to Kazunori Yamahira.

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Ethics approval

The field collections were conducted with permission from RISTEKDIKTI, BRIN, and CEMACS (Research Permit Numbers 394/SIP/FRP/SM/SM/XI/2014 and B-7405/III.5.2/KS01/7/2023). We handled all fish in accordance with the Regulations for Animal Experiments at the University of the Ryukyus, and all the experiments were approved by the Animal Care Committee of University of the Ryukyus (2018099 and 2019084).

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Mokodongan, D.F., Utama, I.V., Nagano, A.J. et al. Rediscovery of Oryzias hubbsi with notes on its reproductive isolation with O. javanicus. Ichthyol Res (2024). https://doi.org/10.1007/s10228-024-00976-y

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