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An assessment of the potamodromous fish Opsariichthys uncirostris uncirostris (Hasu fish) during its reproductive migration to a Lake Biwa tributary using stable isotope ratios and biometric data

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Abstract

Opsariichthys uncirostris uncirostris (Hasu fish), a vulnerable potamodromous fish, is the only piscivorous cyprinid fish in Japan and endemic as a subspecies to Lake Biwa. The species population is on a continued decline for the past 70 years. This study aimed at developing a portfolio on the species during its reproductive migration to Shiotsuo River, a Lake Biwa tributary, by using a combination of biometric measurements and stable isotope ratios in its tissues. Hasu fish were collected monthly, from May to September 2019, using cast nets. The biometric measurements: wet weight, standard length, gonad weight and gut content were collected and used to calculate the gonado-somatic index (GSI) and Fulton’s condition constant (K) and determine the feeding habits of Hasu fish. Carbon and nitrogen isotope ratios (δ13C and δ15N) in slow-changing muscle and fast-changing mucus tissues of Hasu fish were also used to determine recent diet change. At the beginning of the reproductive season, fewer females than males were caught; however, the number of females increased as the season progressed. On average, males were larger than females. Migrating individuals were healthy (K > 1) and over 37% larger in length than those in the 1960s. Gut content analysis revealed, for the first time, Hasu fish feeding in the rivers, primarily on Ayu fish, during the reproductive migration. δ13C and δ15N in muscle and mucus indicated a recent change in diet, i.e., from Lake Biwa to Shiotsuo River, with differences in the onset of feeding (and consequently upstream migration) between sexes and individuals. For the effective conservation of Hasu fish in the other tributaries where Ayu fish traps block other fishes’ migration, we recommend having the rivers open from June to September to cover its variable timing in the recruitment of reproductive individuals.

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Acknowledgments

We thank Dr. H. Sawada for assistance in collecting specimens and providing advice during the stable isotope analysis. We also express our gratitude to Dr. R. Tabata and his team at the Lake Biwa Museum for their guidance and support during the Hasu preliminary survey aquarium experiments. We would also like to thank the Shiga Prefectural Government for giving us permission to collect fish samples in Shiotsuo River. This research was supported by a grant from the Japan Society for the Promotion of Science (JSPS) KAKENHI (Grant No. 16K00630).

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  1. Department of Environmental Solution Technology, Faculty of Science and Technology, Ryukoku University, Seta-Oe, Otsu, Shiga, 520-2194, Japan

    Andrew Mvula & Atsushi Maruyama

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  1. Andrew Mvula
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  2. Atsushi Maruyama
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Correspondence to Andrew Mvula.

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All procedures complied with Japanese laws governing ethical conduct and the care and use of animals in research.

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Mvula, A., Maruyama, A. An assessment of the potamodromous fish Opsariichthys uncirostris uncirostris (Hasu fish) during its reproductive migration to a Lake Biwa tributary using stable isotope ratios and biometric data. Ichthyol Res (2024). https://doi.org/10.1007/s10228-024-00965-1

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  • DOI: https://doi.org/10.1007/s10228-024-00965-1

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