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Note on the banded karyotype of the enigmatic South Asian loach Vaillantella maassi (Cypriniformes: Vaillantellidae)

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Abstract

The karyotype of Vaillantella maassi, a loach with an unusually long dorsal fin, was investigated using sequential CMA3 and AgNO3 staining to test if this character evolved in parallel with Vaillantella’s morphological distinctness. The karyotype was 2n = 50 with 13 pairs of metacentric-submetacentric and 12 pairs of subtelocentric chromosomes. Sequential CMA3 banding and AgNO3 staining evidenced NORs in the short arms of pair no. 2. Additional CMA3-positive, Ag-negative signals were present in the short arms of pairs 14 and 15. The karyotype paralleled those of most other cobitoid loaches studied so far and is apparently not associated with the morphological distinctness of Vaillantella.

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References

  • Buth DG, Dowling TE, Gold JR (1991) Molecular and cytological investigations. In: Winfield IJ, Nelson JS (eds) Cyprinid fishes: systematics, biology and exploitation. Chapman & Hall, London, pp 83–126

    Google Scholar 

  • Howell WM, Black DA (1980) Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia (Basel) 36:1014–1015

    CAS  Google Scholar 

  • Klinkhardt M, Tesche M, Greven H (1995) Database of fish chromosomes. Westarp, Magdeburg

    Google Scholar 

  • Kottelat M (1994) Vaillantella cinnamomea, a new species of balitorid loach from eastern Borneo. Jpn J Ichthyol 40:427–431

    Google Scholar 

  • Nalbant TT (2002) Sixty million years of evolution. Part one: family Botiidae (Pisces: Ostariophysi: Cobitoidea). Trav Mus Hist Nat “Grigore Antipa” 44:309–344

    Google Scholar 

  • Nalbant TT, Bănărescu P (1977) Vaillantellinae, a new subfamily of Cobitidae (Pisces, Cypriniformes). Zool Meded 52:99–105

    Google Scholar 

  • Rábová M, Ráb P, Ozouf-Costaz C (2001) Extensive polymorphism and chromosomal characteristics in a loach fish, Cobitis vardarensis (Ostariophysi, Cobitidae), detected by different banding techniques and flourescence in situ hybridisation. Genetica 111:413–422

    Article  PubMed  Google Scholar 

  • Roberts T (1989) The freshwater fishes of western Borneo (Kalimantan Barat, Indonesia). Mem Calif Acad Sci 14:1–210

    Google Scholar 

  • Saitoh K, Sado T, Mayden LR, Hanzawa N, Nakamura K, Nishida M, Miya M (2006) Mitogenomic evolution and interrelationships of the Cypriniformes (Actinopterygii: Ostariophysi): the first evidence toward resolution of higher-level relationships of the world’s largest freshwater fish clade based on 59 whole mitogenome sequences. J Mol Evol 63:826–841

    Article  PubMed  CAS  Google Scholar 

  • Sawada Y (1982) Phylogeny and zoogeography of the superfamily Cobitoidea (Cyprinoidei, Cypriniformes). Mem Fac Fish Hokkaido Univ 28:65–223

    Google Scholar 

  • Šlechtová V, Bohlen J, Tan HH (2007) Families of Cobitoidea (Teleostei; Cypriniformes) as revealed from nuclear genetic data and the position of the mysterious genera Barbucca, Psilorhynchus, Serpenticobitis and Vaillantella. Mol Phylogenet Evol 44:1358–1365

    Google Scholar 

  • Sola L, Rossi AR, Iaselli V, Rasch EM, Monaco JP (1992) Cytogenetics of bisexual/unisexual species of Poecilia. II. Analysis of heterochromatin and nucleolar organizer regions in Poecilia mexicana mexicana by C-banding and DAPI, quinacrine, chromomycin A3, and silver staining. Cytogenet Cell Genet 60:229–235

    Article  PubMed  CAS  Google Scholar 

  • Suzuki A, Taki Y (1996) Tetraploidisation in the cobitid subfamily Botinae (Pisces, Cypriniformes). Cytobios 85:229–245

    Google Scholar 

  • Völker M, Sonnenberg R, Ráb P, Kullmann H (2006) Karyotype differentiation in Chromaphyosemion killifishes (Cyprinodontiformes, Nothobranchiidae). II: Cytogenetic and mitochondrial DNA analyses demonstrate karyotype differentiation and its evolutionary direction in C. riggenbachi. Cytogenet Genome Res 115:70–83

    Article  PubMed  Google Scholar 

  • Yu X, Zhou T, Li Y, Li K, Zhou M (1989) Chromosomes of Chinese fresh-water fishes. Science Press, Beijing

    Google Scholar 

Download references

Acknowledgments

We thank V. Šlechtová Jr. (Institute of Animal Physiology and Genetics, Liběchov, Czech Republic) and K. Udomritturuj (AquariCorp, Bangkok, Thailand) for their help in the field and K. Saitoh (Tohoku National Fisheries Research Institute, Miyagi, Japan) for his comments on the manuscript. This study was supported by grants no. 206/05/2556 and 206/00/0668 of the Grant Agency of the Czech Republic, grants S5045111 and IAA600450508 of the Grant Agency of the Academy of Sciences of the Czech Republic, and by the IRP IAPG No. AV0Z50450515. The German National Academic Foundation supported M.V.

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Authors and Affiliations

  1. Institute of Animal Physiology and Genetics, Laboratory of Fish Genetics, Academy of Sciences of the Czech Republic, Rumburská 89, 277 21, Liběchov, Czech Republic

    Jörg Bohlen, Marie Rábová & Petr Ráb

  2. Institute of Evolutionary Biology, University of Bonn, An der Immenburg 1, 53121, Bonn, Germany

    Martin Völker

  3. Institute of Animal Physiology and Genetics, Joint Laboratory of Genetics, Physiology and Reproduction of Fishes, Academy of Sciences of the Czech Republic, Rumburská 89, 277 21, Liběchov, Czech Republic

    Petr Ráb

Authors
  1. Jörg Bohlen
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  2. Martin Völker
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  3. Marie Rábová
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  4. Petr Ráb
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Correspondence to Jörg Bohlen.

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Bohlen, J., Völker, M., Rábová, M. et al. Note on the banded karyotype of the enigmatic South Asian loach Vaillantella maassi (Cypriniformes: Vaillantellidae). Ichthyol Res 55, 82–84 (2008). https://doi.org/10.1007/s10228-007-0007-0

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  • DOI: https://doi.org/10.1007/s10228-007-0007-0

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