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Regenerative effects of transplanting autologous mesenchymal stem cells on corticosteroid-induced osteonecrosis in rabbits

  • Original Article
  • Published:
Modern Rheumatology

Abstract

Osteonecrosis (ON) of the femoral head is one of the most serious complications associated with steroid administration. Here, we treated corticosteroid-induced ON in the rabbit by transplanting mesenchymal cells (MCs). Rabbits were injected once with 20 mg/kg of methylprednisolone (MPSL) and divided into three groups as follows: (1) MPSL alone (no further treatment); (2) MPSL+MCs (7 days after MPSL, MCs [1 × 107/2 ml] were injected into the bone marrow cavity of the femurs); (3) MPSL+saline (7 days after MPSL, saline [2 ml] was injected into the bone marrow cavity of the femurs). Subsequently, the incidence of ON in the femurs 4 weeks after MPSL alone and MPSL+saline was 80 and 68.4%, respectively. In contrast, no ON was recorded in rabbits treated with MPSL+MCs. Vascular endothelial growth factor (VEGF) staining and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nick end-labeling (TUNEL) staining was more marked in the MPSL alone and MPSL+saline groups than in the MPSL+MCs rabbits. The percentages of cells in the G1 phase in the MPSL+MCs group were significantly lower than in the other two groups. These findings suggest that the injection of autologous MCs into the femur could prevent corticosteroid-induced ON in patients treated with high-dose short-term steroid medication.

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Acknowledgments

This work was supported by a grant from the Haiteku Research Center of the Ministry of Education, a grant from the Science Frontier Program of the Ministry of Education, Culture, Sports, Science and Technology, and a grant from the 21st Century Center of Excellence (COE) Program of the Ministry of Education, Culture, Sports, Science and Technology.

Conflict of interest

All of the authors confirm that they have no conflicts of interest with regard to this work.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka, 570-8506, Japan

    Hiroyuki Matsuya, Taketoshi Kushida, Taku Asada, Masayuki Umeda, Takahiko Wada & Hirokazu Iida

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  1. Hiroyuki Matsuya
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  2. Taketoshi Kushida
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  3. Taku Asada
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  4. Masayuki Umeda
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  5. Takahiko Wada
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  6. Hirokazu Iida
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Correspondence to Taketoshi Kushida.

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Matsuya, H., Kushida, T., Asada, T. et al. Regenerative effects of transplanting autologous mesenchymal stem cells on corticosteroid-induced osteonecrosis in rabbits. Mod Rheumatol 18, 132–139 (2008). https://doi.org/10.1007/s10165-008-0023-6

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  • DOI: https://doi.org/10.1007/s10165-008-0023-6

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