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Microbial dynamics in the process of restoration of groundwater contaminated by chlorinated ethene in the presence of Escherichia coli

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Abstract

This study investigated the effects of hygiene indicator bacteria during the biostimulation of groundwater contaminated with chlorinated ethene. We showed the state of dechlorination activity and behavior of microbial structure by the addition of Escherichia coli (E. coli) as hygiene indicator bacteria in a contaminated groundwater sample. Dechlorination of tetrachloroethene and trichloroethene to cis-1,2-dichloroethene (cis-DCE) within 14 days took place similarly both with and without the addition of E. coli. This indicated that inhibition of against dechlorinating activity of corresponding dechlorinating bacteria was not caused by E. coli. Structural change of the bacterial community was analyzed both before and after dechlorination using a denaturing gradient gel electrophoresis (DGGE) and clone library. The result of DGGE detected E. coli only at day 0. A sample at day 14 after dechlorination detected Pseudomonas putida, Anaerosinus glycerini, and Clostridium genus but not E. coli. The result of the clone library also showed an identical profile. Detection of E. coli using desoxycholate media was decreased from 2.3 × 106 cells/ml to 6.0 × 103 cells/ml during day 14. These results suggest that biostimulation of groundwater contaminated by chlorinated ethene in the presence of hygiene bacteria caused the dechlorination without activity inhibition and decrease of dechlorinating bacteria.

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Acknowledgments

We gratefully acknowledge Panasonic Environmental System and Engineering Co. Ltd, for providing groundwater sample used in this study. We also express our special thanks to Professor Fuseng Li, River Basin Research Center, Gifu University, for supporting part of this work.

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Authors and Affiliations

  1. United Graduate School of Agricultural Science, Gifu University, Gifu, Japan

    Bhowmik Arpita & Kazuhiro Takamizawa

  2. Faculty of Applied Biological Science, Gifu University, Gifu, Japan

    Koji Ishimura, Kohei Nakamura & Kazuhiro Takamizawa

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  1. Bhowmik Arpita
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  4. Kazuhiro Takamizawa
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Correspondence to Kazuhiro Takamizawa.

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Arpita, B., Ishimura, K., Nakamura, K. et al. Microbial dynamics in the process of restoration of groundwater contaminated by chlorinated ethene in the presence of Escherichia coli . J Mater Cycles Waste Manag 15, 335–341 (2013). https://doi.org/10.1007/s10163-013-0124-y

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