Abstract
We describe how a novel thermosensitive escape behaviour in Drosophila larvae can be used to teach neurobiology principles to both undergraduates and school children. The assays are inexpensive, robust and reliably accurate employing apparatus readily available in most science classrooms. The use of Drosophila avoids the employment of vertebrate models and the ethical and expense issues that this entails. We use this practical to effectively teach the principals of calibration and neural sensory responses to post-16-year-old students and undergraduate students.
References
Animal Welfare Act (Laboratory Animal Welfare) Act 1966, P.L. 89–544 (1966)
Animals (Scientific Procedures) Act 1986 (1986)
Babcock DT, Landry C, Galko MJ (2009) Cytokine signaling mediates UV-induced nociception sensitization in Drosophila larvae. Curr Biol 19:799–806
Carew TJ, Walters ET, Kandel ER (1981) Associative learning in Aplysia: cellular correlates supporting a conditioned fear hypothesis. Science 211:501–504
Hodgkin AL, Huxley AF (1952a) Currents carried by the sodium and potassium ions through the membrane of the giant axon of Loligo. J Physiol 116:449–472
Hodgkin AL, Huxley AF (1952b) The components of membrane conductance in the giant axon of Loligo. J Physiol 116:473–496
Hodgkin AL, Huxley AF (1952c) The dual effect of membrane potential on sodium conductance in the giant axon of Loligo. J Physiol 116:497–506
Hodgkin AL, Huxley AF (1952d) A quantitative description of membrane current and it’s application to conduction and excitation in nerve. J Physiol 116:507–544
Levine RB, Truman JW (1983) Peptide activation of a simple neural circuit. Brain Res 279:335–338
Oswald M, Rymarczyck B, Chatters A, Sweeney ST (2011) A novel thermosensitive escape behaviour in Drosophila larvae. Fly 5(4). http://www.landesbioscience.com/journals/fly/article/17810/
Stuart AE, Oertel D (1978) Neuronal properties underlying processing of visual information in the barnacle. Nature 275:287–290
Tharp GD, Woodman DA (2011) Experiments in physiology. Benjamin Cummings, New York
Tracey WD, Wilson RI, Laurent G, Benzer S (2003) Painless, a Drosophila gene essential for nociception. Cell 113:261–273
Acknowledgments
Eileen Gilhooly and the sixth form Biology Class from King James’s School Knaresborough who tried this practical first. Trevor Illingworth, York Biology Electronics Workshops for amplifier manufacture. The authors would also like to thank BBSRC for support through the Regional Schools Champion Scheme.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Harrison, A.B., Oswald, M. & Sweeney, S.T. Teaching report: the use of Drosophila melanogaster larval thermosensitive escape behaviour as a model system to demonstrate sensory function. Invert Neurosci 11, 109–112 (2011). https://doi.org/10.1007/s10158-011-0123-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10158-011-0123-4