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Proteomic analysis indicates altered expression of plasma proteins in a rat nephropathy model

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Abstract

Background

Minimal-change nephrotic syndrome is an idiopathic disease in which protein leaks through podocytes into the urine. We used proteomic tools to examine differences of plasma protein expression in healthy rats and rats with doxorubicin-induced nephropathy treated with or without prednisone.

Methods

Healthy three-month-old Sprague–Dawley male rats were randomly chosen for one injection of doxorubicin (5.5 mg/kg) through the caudal vein to induce nephropathy (n = 50) or the same volume of saline (control, n = 20). After 1 week, 25 rats in the nephropathy group received topical prednisone (5.5 mg/kg/day) for 21 days and another 25 rats (untreated nephropathy) and the control rats received topical water. At 4 weeks, protein chips generated from rat plasma samples were analyzed by surface enhanced laser desorption/ionization–time of flight mass spectrometry (SELDI–TOF–MS) to obtain mass-to-charge ratios (m/z) of proteins of 2–50 kDa.

Results

Relative to control rats, untreated nephropathic rats had four significantly higher and seven significantly lower m/z peaks. Prednisone treatment significantly normalized the intensities of peaks 9069 and 15005 (which correspond to cortexin-1 and interleukin-17A, respectively, according to Swiss Prot database) by increasing the expression of 9069 but reducing expression of 15005.

Conclusion

Significant differences in plasma proteins can be identified by proteomic analysis using SELDI–TOF–MS in a rat model of nephropathy.

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Acknowledgments

The study was supported by the Natural Science Foundation of Fujian (project numbers 2007J0307, 2008J0093).

Conflict of interest

The authors have declared that no conflict of interest exists.

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Authors and Affiliations

  1. Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Huatuo Road No.1 Minhou County, Fuzhou, 350108, China

    Si Ai & Jian Zheng

  2. Division of Pediatric Nephrology, Fujian University of Traditional Chinese Medicine, People Hospital Affiliated to Fujian University of Traditional Chinese Medicine, 817 Road No. 602, Fuzhou, 350004, Fujian, China

    Si Ai, Jian Zheng, Qing Lin & Rongyan Chen

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  1. Si Ai
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  2. Jian Zheng
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  3. Qing Lin
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Correspondence to Jian Zheng.

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Ai, S., Zheng, J., Lin, Q. et al. Proteomic analysis indicates altered expression of plasma proteins in a rat nephropathy model. Clin Exp Nephrol 17, 24–31 (2013). https://doi.org/10.1007/s10157-012-0662-y

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  • DOI: https://doi.org/10.1007/s10157-012-0662-y

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