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Altered monocyte-derived dendritic cell function in patients on hemodialysis: a culprit for underlying impaired immune responses

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Abstract

Background

Patients with end-stage renal disease (ESRD) are known to have impaired immune function. Dendritic cells (DCs) are the major antigen-presenting cells that initiate primary immune responses, linking innate and adaptive immunity. Although suboptimal immune responses to vaccination, as frequently observed in ESRD patients, might suggest the presence of impaired DC function, the precise nature of altered DC function is not fully understood.

Methods

In the current study, we compared the maturation status, viability, and function of monocyte-derived DCs (moDCs) of patients on hemodialysis (HD) with healthy controls.

Results

Surface expression of major histocompatibility complex class II, CD83, and CD86, and chemokine receptor CCR7 in moDCs was not different between HD patients and healthy controls. No significant difference was detected in the viability of moDCs determined by expression of annexin V and propidium iodide between two groups. However, moDCs from HD patients produced significantly higher amounts of IL-6 when stimulated by cytokine cocktails compared to healthy controls. In addition, mature moDCs from HD patients showed significantly enhanced allogeneic T-cell proliferation compared to healthy controls.

Conclusions

Our data demonstrate aberrant DC function in HD patients and suggest that this might contribute to impaired immune responses.

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Acknowledgments

We thank Mrs. Yoon Sook Ko and Mrs. Hee Young Lee for technical assistance in these experiments.

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Authors and Affiliations

  1. Division of Nephrology, Department of Internal Medicine, Korea University Medical College, Seoul, Korea

    Hye Min Choi, Young Seok Woo, Myung Gyu Kim, Sang-Kyung Jo, Won Yong Cho & Hyoung Kyu Kim

  2. Department of Internal Medicine, Korea University Anam Hospital, 5Ka, Anam-Dong, Sungbuk-Gu, Seoul, 136-705, Korea

    Sang-Kyung Jo

Authors
  1. Hye Min Choi
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  2. Young Seok Woo
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  3. Myung Gyu Kim
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  4. Sang-Kyung Jo
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  5. Won Yong Cho
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  6. Hyoung Kyu Kim
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Correspondence to Sang-Kyung Jo.

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Choi, H.M., Woo, Y.S., Kim, M.G. et al. Altered monocyte-derived dendritic cell function in patients on hemodialysis: a culprit for underlying impaired immune responses. Clin Exp Nephrol 15, 546–553 (2011). https://doi.org/10.1007/s10157-011-0424-2

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  • DOI: https://doi.org/10.1007/s10157-011-0424-2

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