Abstract
Background
The present study is aiming at elucidating the effect of intraoperative lavage with short-chain fatty acids (SCFAs) on colonic anastomosis in rats.
Methods
Forty male Wistar rats were randomized into four groups (10 rats each). After resection of a segment of transverse colon, an end-to-end anastomosis was performed. In the 1st group, no intraoperative large bowel lavage was performed; in the 2nd, a lavage with normal saline solution; in the 3rd, the animals received a diet rich in SCFAs pre- and postoperatively, and a lavage with normal saline was performed; and in the 4th group, an intraoperative lavage with SCFAs was carried out. On the 4th postoperative day, the animals were sacrificed. Septic complications, adhesions and anastomoses were graded macroscopically and histologically, and bursting pressure of the anastomoses, CRP, IL-6 and TNF-a was measured.
Results
Fewer septic complications (abscesses and minimal ruptures) and adhesions were observed in the 4th group with the intraoperative lavage with SCFAs. The bursting pressure also, in the same group, was higher (73.3 mmHg), followed by the 1st group (67.1 mmHg).
Conclusion
Intraoperative lavage with SCFAs increases the bursting pressure of colonic anastomoses, while lavage with saline solution decreases it, in comparison to the group without lavage.
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References
Kanellos I, Blouhos K, Demetriades H et al (2004) The failed intraperitoneal colon anastomosis after colon resection. Tech Coloproctol 8:s53–s55
Mantzoros I, Kanellos I, Angelopoulos S et al (2006) The effect of insulin-like growth factor I on healing of colonic anastomoses in cortisone-treated rats. Dis Colon Rectum 49:1431–1438
Apostolidis S, Michalopoulos A, Hytiroglou P et al (2000) Prevention of blood transfusion induced impairment of anastomotic healing by leukocyte depletion in rats. Eur J Surg 166(7):562–567
Apostolidis SA, Papadopoulos VN, Michalopoulos AA et al (2004) The effects of ranitidine on anastomotic healing. Experimental study. Tech Coloproctol 8S:104–107
Mantzoros I, Kanellos I, Demetriades H et al (2004) Effects of steroid on the healing of colonic anastomoses in the rat. Tech Coloproctol 8:s180–s183
Kanellos I, Christoforidis E, Kanellos D et al (2006) The healing of colon anastomosis covered with fibrin glue after early postoperative intraperitoneal chemotherapy. Tech Coloproctol 10:115–120
Rolandelli RH, Koruda MJ, Settle G, Rombeau JL (1986) Effects of intraluminal infusion of short- chain fatty acids on the healing of colonic anastomosis in the rat. Surgery 100:198–204
Terzi C, Sevinç AB, Koçdor H et al (2004) Improvement of colonic healing by preoperative rectal irrigation with short-chain fatty acids in rats given radiotherapy. Dis Colon Rectum 47:2184–2194
Kanellos D, Blouhos K, Pramateftakis MG et al (2007) Effect of 5-fluorouracil plus interferon on the integrity of colonic anastomoses covering with fibrin glue. World J Surg 31:186–191
Slavin J, Nash JR, Kingsnorth NA (1992) Effect of transforming growth factor beta and basic fibroblast growth factor on steroid-impaired healing intestinal wounds. Br J Surg 79:69–72
Martin CW, Muir IF (1990) The role of lymphocytes in wound healing. Br J Plast Surg 43:655–662
Matsumoto S, Imaeda Y, Umemoto S et al (2002) Cimetidine increases survival of colorectal cancer patients with high levels of sialyl Lewis-X and sialyl Lewis-A epitope expression on tumor cells. Br J Cancer 86:161–166
Zacharakis E, Demetriades H, Kanellos D et al (2007) Contribution of insulin-like growth factor I to the healing of colonic anastomoses in rats. J Invest Surg 20:9–14
Ishimura K, Moroguchi A, Okano K et al (2002) Local expression of tumor necrosis factor and interleukin-10 on wound healing of intestinal anastomosis during endotoxemia in mice. J Surg Res 108:91–97
Vilcek J, Palombella VJ, Henriksen-DeStefano D et al (1986) Fibroblast growth enhancing activity of tumor necrosis factor and its relationship to other polypeptide growth factors. J Exp Med 163:632–643
Buck M, Houglum K, Chojkier M (1996) Tumor necrosis factor inhibits collagen 1 gene expression and wound healing in a murine model of cachexia. Am J Pathol 149:195–197
Kanellos D, Pramateftakis MG, Demetriades H et al (2008) Healing of colonic anastomoses after immediate postoperative intraperitoneal administration of oxaliplatin. Int J Colorectal Dis 23:1185–1191
Zacharakis E, Demetriades H, Pramateftakis MG et al (2008) Effect of IGF-I on healing of colonic anastomoses in rats under 5-FU treatment. J Surg Res 144:138–144
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Netta, S., Michalopoulos, A., Apostolidis, S. et al. Enhancement of colonic anastomotic strength in rats by short-chain fatty acids. Tech Coloproctol 14 (Suppl 1), 53–55 (2010). https://doi.org/10.1007/s10151-010-0611-2
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DOI: https://doi.org/10.1007/s10151-010-0611-2