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Immunohistochemical location of the p75 neurotrophin receptor (p75NTR) in oral leukoplakia and oral squamous cell carcinoma

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Abstract

Background

Recent studies have demonstrated that the p75 neurotrophin receptor (p75NTR) is a useful marker of keratinocyte stem cells. Although the stem cell markers of original normal tissue have been used to identify cancer stem cells in a variety of cancers, the expression and function of p75NTR have been poorly understood in oral squamous cell carcinoma (OSCC). The objective of this study is, thus, to examine p75NTR expression immunohistochemically in oral leukoplakia (OL), the most frequent precancerous lesion, and OSCC, and to reveal the usefulness of p75NTR as a marker for undifferentiated cancer cells and a novel prognostic factor for OSCC patients.

Methods

In this study immunohistochemical expression of p75NTR, Ki-67, cytokeratin (CK) 5, and CK14 was examined in 112 cases of OL and 81 of OSCC. The labeling indices (LIs) of p75NTR and Ki-67 were calculated, and the association of these LIs with histopathologic characteristics was then evaluated.

Results

In the normal oral epithelium and OL, p75NTR was expressed only in the basal layer, and its LI was invariant, irrespective of the extent of epithelial dysplasia. In OSCC, however, p75NTR-LI was significantly increased in association with upgrading of histologic grade and mode of tumor invasion. Furthermore, the prognosis of the high p75NTR-LI group (LI ≥ 53.1%) was poorer than that of the low p75NTR-LI group (LI < 53.1%).

Conclusions

These results suggest that p75NTR is expressed in undifferentiated cell populations in OL and OSCC. Furthermore, p75NTR is possibly involved in invasion and poor prognosis in OSCC.

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Acknowledgments

The work was supported by a Grant-in-Aid (no. 20791531) from the Japanese Ministry of Education, Culture, Science, Sports and Technology of Japan

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Section of Oral and Maxillofacial Oncology, Division of Maxillofacial Diagnostic and Surgical Sciences, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan

    Takahiro Kiyosue, Shintaro Kawano, Ryota Matsubara, Yuichi Goto, Mitsuhiro Hirano, Teppei Jinno, Takeshi Toyoshima, Ryoji Kitamura, Kazunari Oobu & Seiji Nakamura

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  1. Takahiro Kiyosue
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  2. Shintaro Kawano
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  3. Ryota Matsubara
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  5. Mitsuhiro Hirano
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Correspondence to Shintaro Kawano.

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Kiyosue, T., Kawano, S., Matsubara, R. et al. Immunohistochemical location of the p75 neurotrophin receptor (p75NTR) in oral leukoplakia and oral squamous cell carcinoma. Int J Clin Oncol 18, 154–163 (2013). https://doi.org/10.1007/s10147-011-0358-4

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  • DOI: https://doi.org/10.1007/s10147-011-0358-4

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