Abstract
SOS2-like protein kinases (PKS/CIPK) family genes are known to be involved in various abiotic stresses in plants. Even though, its functions have been well characterized under salt and drought stresses. The roles of PKS genes associated with alkaline stress response are not fully established yet. In this study, we identified 56 PKS family genes which could be mainly classified into three groups in wild soybean (Glycine soja). PKS family genes transcript profiles revealed different expression patterns under alkali stress. Furthermore, we confirmed the regulatory roles of GsPKS24 in response to NaHCO3, pH and ABA treatments. Overexpression of GsPKS24 enhanced plant tolerance to pH stress in Arabidopsis and soybean hairy roots but conferred suppressed pH tolerance in Arabidopsis atpks mutant. Additionally, Overexpression of GsPKS24 decreased the ABA sensitivity compared to Arabidopsis atpks mutant which displayed more sensitivity towards ABA. Moreover, upregulated expression of stress responsive and ABA signal-related genes were detected in GsPKS24 overexpression lines. In conclusion, we identified the wild soybean PKS family genes, and explored the roles of GsPKS24 in positive response to pH stress tolerance, and in alleviation of ABA sensitivity.
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The datasets generated and analyzed during the current study is available from the corresponding authors on reasonable request.
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Acknowledgements
The authors would like to thank Researchers Supporting Project number (RSP2023R118), King Saud University, Riyadh, Saudi Arabia. The authors thank the Heilongjiang Provincial Academy of Agricultural Sciences for providing soybean seeds.
Funding
This work was supported by the Natural and Science Foundation of China (grant number 32001454), Natural Science Foundation of Heilongjiang Province (grant number YQ2023C034) and Researchers Supporting Project number (grant number RSP2023R118), King Saud University.
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XM, DD and CC performed the experiment, analyzed data; DD and CC wrote the manuscript; JW and ZN edited the manuscript; HE, LJ, CC and XJ provided ideas, designed the research; all authors read and approved the final manuscript.
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Ding, D., Mi, X., Wu, J. et al. GsPKS24, a calcineurin B-like protein-interacting protein kinase gene from Glycine soja, positively regulates tolerance to pH stress and ABA signal transduction. Funct Integr Genomics 23, 276 (2023). https://doi.org/10.1007/s10142-023-01213-x
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DOI: https://doi.org/10.1007/s10142-023-01213-x