Synthesis of Hemolymph High-Density Lipoprotein β-Glucan Binding Protein by Penaeus vannamei Shrimp Hepatopancreas

Abstract:

It was recently shown that the shrimp high-density lipoprotein (HDL) and the β-1,3-glucan binding protein (BGBP) are identical, implying dual functions for the same protein: lipid transport and involvement in the defense system. Because this protein is present in plasma, and the hepatopancreas is a major lipid storage gland, we investigated the presence of the HDL/BGBP polypeptide and its messenger RNA in this tissue using a monospecific antibody against HDL/BGBP. Hepatopancreas crude protein extracts, as well as polypeptides produced by poly(A)⁺ RNA in vitro translation, were recognized by the anti-HDL/BGBP. Furthermore, a specific pattern was revealed in hepatopancreas thin sections by immunodetection. Strong recognition was seen in the epithelial cells of hepatopancreatic tubules, probably related to the secretion process of this protein.