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Localization and possible function of nrF-AGP, an alpha-1-acid glycoprotein-like protein in viviparous fish Neoditrema ransonnetii (Perciformes, Embiotocidae)

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Abstract

The nrF-AGP, a 51-kDa acidic glycoprotein found in surfperch (Neoditrema ransonnetii; Perciformes, Embiotocidae), is a member of the lipocalin superfamily. nrF-AGP is the major component in ovarian cavity fluid (OCF), but not in plasma of pregnant females, which suggests its potential relevance in pregnancy. However, its production in the liver, irrespective of reproductive cycle and sex, indicates that the protein also has physiological functions other than its contribution to reproduction. In the present study, Western blot analysis indicated that this protein is widely distributed in the cutaneous and intestinal mucosa, bile, and abdominal adipose tissue of fish, as well as plasma and OCF. Immunohistochemical staining of nrF-AGP was observed in hepatocytes, adipocytes, pancreatic cells, epidermal cells, and epithelial cells of ovigerous lamellae. Transcripts were detected in adipose tissue as well as hepatocytes by reverse transcription PCR analysis. This broad distribution of nrF-AGP suggests that this protein participates in various biological processes through its ability to bind to hydrophobes. After administration of biotinylated F-AGP into the ovarian cavity, the protein was detected in the cytoplasm of the intestinal epithelial cells of the fetus within 4 h. This suggests that nrF-AGP in the ovarian cavity acts as a transporter delivering maternal resources to the fetus.

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Authors and Affiliations

  1. School of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa, 252-0373, Japan

    Osamu Nakamura, Yuki Watabe, Naoko Matsumoto, Osamu Takasugi, Ayako Watanabe & Shigeyuki Tsutsui

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  1. Osamu Nakamura
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  2. Yuki Watabe
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  3. Naoko Matsumoto
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  4. Osamu Takasugi
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  5. Ayako Watanabe
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  6. Shigeyuki Tsutsui
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Correspondence to Osamu Nakamura.

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Nakamura, O., Watabe, Y., Matsumoto, N. et al. Localization and possible function of nrF-AGP, an alpha-1-acid glycoprotein-like protein in viviparous fish Neoditrema ransonnetii (Perciformes, Embiotocidae). Fish Physiol Biochem 40, 1907–1915 (2014). https://doi.org/10.1007/s10695-014-9978-8

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  • DOI: https://doi.org/10.1007/s10695-014-9978-8

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