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Effect of Astaxanthin on the Antioxidant Capacity and Intestinal Microbiota of Tsinling Lenok Trout (Brachymystax lenok tsinlingensis)

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Abstract

Astaxanthin (Ast) has been shown to be beneficial for the antioxidant capacity, immune system, and stress tolerance of fish. This study was conducted to investigate the effects of dietary supplementation of Ast on the antioxidant capacity and intestinal microbiota of tsinling lenok trout. We formulated four diets with 0 (CT), 50 (A50), 100 (A100), and 150 (A150) mg/kg Ast. The results showed that Ast increased total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px), lysozyme (LZM), and catalase (CAT) activities. Malondialdehyde (MDA) content was lower in A150 and A100 than in CT (P < 0.05). Furthermore, the activities of acid phosphatase (ACP) were higher in A100 and A150 than in CT (P < 0.05). We harvested the midgut and applied next-generation sequencing of 16S rDNA. Compared to the control group, the Ast group had a greater abundance of Halomonas. Functional analysis showed that polycyclic aromatic hydrocarbon degradation was significantly higher with Ast, while novobiocin biosynthesis and C5-branched dibasic acid metabolism were significantly lower with Ast. In conclusion, Ast could enhance the antioxidant capacity, non-specific immunity, and intestinal health of tsinling lenok trout.

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Data Availability

The datasets are available from the corresponding author on reasonable request.

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Funding

This work was supported by the Natural Science Foundation of Gansu Province (No. 21JR7RE178), and Double First-Class Key Projects in Gansu Province (No. GSSYLXM-08).

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  1. Key Laboratory of Resource Utilization of Agricultural Solid Waste in Gansu Province, Tianshui Normal University, South Xihe Road, Qinzhou District, Tianshui, 741000, Gansu Province, People’s Republic of China

    Fang Ma, Ruilin Ma, Yali Zou & Lei Zhao

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  1. Fang Ma
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Contributions

F.M. and R.L. conceived and designed the research. F.M., R.L., and Y.L. performed experiments. F.M. and L.Z. analyzed data. F.M. wrote the manuscript. All authors contributed to the discussion and review of the manuscript.

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Correspondence to Fang Ma.

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All the methods used in this study were carried out according to the guiding principles of the Chinese Legislation on the Use and Care of Laboratory Animals. The protocol was approved by the Institutional Ethic Committee of Tianshui Normal University (TSNU).

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Ma, F., Ma, R., Zou, Y. et al. Effect of Astaxanthin on the Antioxidant Capacity and Intestinal Microbiota of Tsinling Lenok Trout (Brachymystax lenok tsinlingensis). Mar Biotechnol 24, 1125–1137 (2022). https://doi.org/10.1007/s10126-022-10175-3

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