Abstract
Antimicrobial peptides (AMPs) are an important part of non-specific immunity and play a key role in the cellular host defense against pathogens and tissue injury infections. We investigated the effects of AMP supplementation on the antioxidant capacity, non-specific immunity, and gut microbiota of tsinling lenok trout. 240 fish were fed diets (CT, A120, A240 and A480) containing different amounts of AMP peptides (0, 120 mg kg−1, 240 mg kg−1, 480 mg kg−1) for 8 weeks. Our results showed that the activity of total antioxidant capacity (T-SOD) and glutathione peroxidase (GSH-Px), lysozyme (LZM), catalase (CAT) and acid phosphatase (ACP) in the A240 and A480 group were higher than that in the CT group (P < 0.05). The content of malondialdehyde (MDA) in AMP group was significantly lower than that in CT group (P < 0.05). Furthermore, we harvested the mid-gut and applied next-generation sequencing of 16S rDNA. The results showed that the abundance of Halomonas in AMP group was significantly lower than that in CT group. Functional analysis showed that the abundance of chloroalkane and chloroalkene degradation pathway increased significantly in AMP group. In conclusion, AMP enhanced the antioxidant capacity, non-specific immunity, and intestinal health of tsinling lenok trout.
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The data which support the result of this study are available from the corresponding author upon reasonable request.
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Funding
This work was supported by the National Natural Science Foundation of China (No. 32260258), Natural Science Foundation of Gansu Province (No. 21JR7RE178); Double first-class key projects in Gansu Province (No. GSSYLXM-08).
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F.M., and R.L. conceived and designed research. F.M., R.L., and L.Z. performed experiments. F.M., and L.Z. analyzed data. F.M. wrote the manuscript. All authors contributed to discussion and review of the manuscript.
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Ma, F., Ma, R. & Zhao, L. Effects of Antimicrobial Peptides on Antioxidant Properties, Non-specific Immune Response and Gut Microbes of Tsinling Lenok Trout (Brachymystax lenok tsinlingensis). Biochem Genet (2024). https://doi.org/10.1007/s10528-024-10708-6
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DOI: https://doi.org/10.1007/s10528-024-10708-6