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Gene Co-Expression Network Analysis Reveals the Correlation Patterns Among Genes in Different Temperature Stress Adaptation of Manila Clam

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Abstract

The Manila clam (Ruditapes philippinarum) is one of the most important aquaculture species and widely distributed along the coasts of China, Japan, and Korea. Due to its wide distribution, it can tolerate a wide range of temperature. Studying the gene expression profiles of clam gills had found differentially expressed genes (DEGs) and pathway involved in temperature stress tolerance. A systematic study of cellular response to temperature stress may provide insights into the mechanism of acquired tolerance. Here, weighted gene co-expression network analysis (WGCNA) was carried out using RNA-seq data from gill transcriptome in response to high and low temperature stress. There are a total 32 gene modules, of which 18 gene modules were identified as temperature-related modules. Blue module was one significantly correlated with temperature which was associated with cellular metabolism, apoptosis pathway, ER stress, and others.

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Acknowledgements

This work was supported by the Chinese Ministry of Science and Technology through the National Key Research and Development Program of China (2018YFD0901400, 2019YFD0900704) and supported by China Agriculture Research System of MOF and MARA, the Intercollegiate Joint training Program of Colleges and Universities in Liaoning Province, and the Scientific Research funding from Liaoning Provincial Department of Education (LJKZ0701).

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Authors and Affiliations

  1. Engineering and Technology Research Center of Shellfish Breeding in Liaoning Province, College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China

    Kifat Jahan, Zhihui Yin, Yanming Zhang, Xiwu Yan & Hongtao Nie

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  1. Kifat Jahan
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  2. Zhihui Yin
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  3. Yanming Zhang
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  4. Xiwu Yan
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  5. Hongtao Nie
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Correspondence to Hongtao Nie.

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Jahan, K., Yin, Z., Zhang, Y. et al. Gene Co-Expression Network Analysis Reveals the Correlation Patterns Among Genes in Different Temperature Stress Adaptation of Manila Clam. Mar Biotechnol 24, 542–554 (2022). https://doi.org/10.1007/s10126-022-10117-z

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