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Comparative Transcriptome Analysis Reveals Differentially Expressed Genes and Signaling Pathways Between Male and Female Red-Tail Catfish (Mystus wyckioides)

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Abstract

Sexual dimorphism is widespread in fish species. The red-tail catfish (Mystus wyckioides) is a commercially important catfish in the lower reaches of the Lancang River and the Mekong basin, and it shows a growth advantage in males. Here, RNA-seq was for the first time used to explore the gene expression difference between the sexes in the hypothalamus and pituitary of red-tail catfish, respectively. In the hypothalamus, 5732 and 271 unigenes have significantly higher and lower expressions, respectively, in males compared with females. KEGG analysis showed that 212 DEGs were annotated to 216 signaling pathways, and enrichment analysis suggested different levels of cAMP and glutamatergic synapse signaling between male and female hypothalami and some of the DEGs appear involved in gonad development and growth. In the pituitary, we found only 19 differentially expressed unigenes, which were annotated to 32 signaling pathways, most of which play important roles in gonad development.

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Funding

This work was supported by National Key R&D Program of China (2018YFD0901201), the Key Program of Frontier Sciences of the Chinese Academy of Sciences (QYZDY-SSW-SMC025), the Yunnan Academician Workstation (Y73Z04-1-301), the earmarked fund for Modern Agro-industry Technology Research System (NYCYTX-49), and the Autonomous Project of the State Key Laboratory of Freshwater Ecology and Biotechnology (2016FBZ01).

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  1. Jun-Jie Wu and Yu-Lin Zhou contributed equally to this work.

Authors and Affiliations

  1. Yunnan Institute of Fishery Sciences Research, Kunming, 650111, China

    Jun-Jie Wu, Guang-Hua Li, Fang-Peng Jin, Li-Li Cui & Hai-Tao Gao

  2. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, University of the Chinese Academy of Sciences, Wuhan, 430072, China

    Yu-Lin Zhou, Zhong-Wei Wang, Li Zhou & Jian-Fang Gui

  3. Xishuangbanna Native Fish Research and Breeding Center, Xishuangbanna, 666100, China

    Xin-Ping Li

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  1. Jun-Jie Wu
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Supplementary Fig. 1

Length distribution of the de novo assembled unignes. (PNG 487 kb)

High resolution image

(TIF 2665 kb)

Supplementary Fig. 2

Stastics of species distribution of Blast hits and annotated unigenes against NR, KOG, GO, KEGG and SWISSPROT database. (A), Stastics of annotated unigenes in hypothalamus. (B), Stastics of annotated unigenes in pituitary. (C), Species distribution of hypothalamic Blast hits against NR database. (D), Species distribution of pituitary Blast hits against NR database. (PNG 2711 kb)

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(TIF 62438 kb)

Supplementary Fig. 3

Linear regression analysis of expression data generated by RNA-seq and qRT-PCR. X-axis represents log2(mean normalized RPKM), Y-axis represents log2(mean normalized qRT-PCR). (PNG 212 kb)

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(TIF 8647 kb)

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Wu, JJ., Zhou, YL., Wang, ZW. et al. Comparative Transcriptome Analysis Reveals Differentially Expressed Genes and Signaling Pathways Between Male and Female Red-Tail Catfish (Mystus wyckioides). Mar Biotechnol 21, 463–474 (2019). https://doi.org/10.1007/s10126-019-09894-x

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