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Marine Microorganisms for Biocatalysis: Selective Hydrolysis of Nitriles with a Salt-Resistant Strain of Meyerozyma guilliermondii

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Abstract

A screening among marine yeasts was carried out for nitrile hydrolyzing activity. Meyerozyma guilliermondii LM2 (UBOCC-A-214008) was able to efficiently grow on benzonitrile and cyclohexanecarbonitrile (CECN) as sole nitrogen sources. A two-step one-pot method for obtaining cells of M. guilliermondii LM2 (UBOCC-A-214008) endowed with high nitrilase activity was established; the resulting whole cells converted different nitriles with high molar conversions and showed interesting enantioselectivity toward racemic substrates. Nitrilase from M. guilliermondii LM2 (UBOCC-A-214008) displayed high activity on aromatic substrates, but also arylaliphatic and aliphatic substrates were accepted. Salt-resistant M. guilliermondii LM2 (UBOCC-A-214008) was used in media with different salinity, being highly active up to 1.5 M NaCl concentration. Finally, hydrolysis of nitriles was efficiently performed using a bioprocess (yeast growth and biotransformation with resting cells) entirely carried out in seawater.

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Acknowledgements

The authors acknowledge G. Burgaud (University of Brest) for kindly providing the yeast strains.

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Authors and Affiliations

  1. Department of Food, Environmental and Nutritional Sciences (DeFENS), University of Milan, Via L. Mangiagalli 25, Milan, Italy

    Immacolata Serra, Claudia Capusoni, Francesco Molinari, Loana Musso, Luisa Pellegrino & Concetta Compagno

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  1. Immacolata Serra
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  2. Claudia Capusoni
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  3. Francesco Molinari
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  4. Loana Musso
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  5. Luisa Pellegrino
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  6. Concetta Compagno
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Correspondence to Immacolata Serra.

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Serra, I., Capusoni, C., Molinari, F. et al. Marine Microorganisms for Biocatalysis: Selective Hydrolysis of Nitriles with a Salt-Resistant Strain of Meyerozyma guilliermondii. Mar Biotechnol 21, 229–239 (2019). https://doi.org/10.1007/s10126-019-09875-0

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