Abstract
2-Cyanopyridine proved to act as a powerful nitrilase inducer in Aspergillus niger K10, Fusarium solani O1, Fusarium oxysporum CCF 1414, Fusarium oxysporum CCF 483 and Penicillium multicolor CCF 2244. Valeronitrile also enhanced the nitrilase activity in most of the strains. The highest nitrilase activities were produced by fungi cultivated in a Czapek-Dox medium with both 2-cyanopyridine and valeronitrile. The specific nitrilase activities of these cultures were two to three orders of magnitude higher than those of cultures grown on other nitriles such as 3-cyanopyridine or 4-cyanopyridine.
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Acknowledgments
Financial support of this work by the grants IAA4020213 (Grant Agency of the Academy of Sciences of the Czech Republic), 203/05/2267 (Czech Science Foundation), OC D25.001 (Ministry of Education, Czech Republic) and the institutional research concept AVOZ50200510 (Institute of Microbiology) is gratefully acknowledged.
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Kaplan, O., Vejvoda, V., Charvátová-Pišvejcová, A. et al. Hyperinduction of nitrilases in filamentous fungi. J Ind Microbiol Biotechnol 33, 891–896 (2006). https://doi.org/10.1007/s10295-006-0161-9
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DOI: https://doi.org/10.1007/s10295-006-0161-9