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Design and Performance of a Turbot (Scophthalmus maximus) Oligo-microarray Based on ESTs from Immune Tissues

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Abstract

An expressed sequence tag database from immune tissues was used to design the first high-density turbot (Scophthalmus maximus) oligo-microarray with the aim of identifying candidate genes for tolerance to pathogens. Specific oligonucleotides (60 mers) were successfully designed for 2,716 out of 3,482 unique sequences of the database. An Agilent custom oligo-microarray 8 × 15 k (five replicates/gene; eight microarrays/slide) was constructed. The performance of the microarray and the sources of variation along microarray analysis were examined on spleen pools of controls and Aeromonas salmonicida-challenged fish at 3 days postinfection. Only 48 out of 2,716 probes did not show signal of hybridization on the 32 microarrays employed, thus demonstrating the consistency of the bioinformatic applications of our database. An asymmetric hierarchical design was employed to ascertain the noise associated with biological and technical (RNA extraction, labeling, hybridization, slide, and dye bias) factors using 1C and 2C labeling approaches. The high correlation coefficient between replicates at most factors tested demonstrated the high reproducibility of the signal. An analysis of random-effects variance revealed that technical variation was mostly negligible, and biological variation represented the main factor, even using pooled samples. One-color approach performed at least as well as 2C, suggesting their usefulness due to its higher design flexibility and lower cost. A relevant proportion of genes turn out to be differentially labeled depending on fluorophore, which alerts for the likely need of swapping replication in 2C experiments. A set of differentially expressed genes and enriched functions related to immune/defense response were detected at 3 days postchallenging.

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References

  • Allison DB, Cui XQ, Page GP, Sabripour M (2006) Microarray data analysis: from disarray to consolidation and consensus. Nat Rev Genet 7:55–65

    Article  PubMed  CAS  Google Scholar 

  • Avendaño-Herrera R, Núñez S, Barja JL, Toranzo AE (2008) Evolution of drug resistance and minimum inhibitory concentration to enrofloxacin in Tenacibaculum maritimum strains isolated in fish farms. Aquacult Int 16:1–11

    Article  CAS  Google Scholar 

  • Bouza C, Hermida M, Pardo BG, Fernández C, Castro J, Fortes G, Sánchez L, Presa P, Pérez M, Sanjuán A, Comesaña S, Álvarez JA, Calaza M, Cal R, Piferrer F, Martínez P (2007) A microsatellite genetic map in the turbot (Scophthalmus maximus). Genetics 177:2457–2467

    Article  PubMed  CAS  Google Scholar 

  • Bouza C, Hermida M, Millán A, Vilas R, Vera M, Fernández C, Calaza M, Pardo BG, Martínez P (2008) Characterization of EST-derived microsatellites for gene mapping and evolutionary genomics in turbot. Anim Genet 39:666–670

    Article  PubMed  CAS  Google Scholar 

  • Brown SJ, Kuhn D, Wisser R, Power E, Schnell R (2004) Quantification of sources of variation and accuracy of sequence discrimination in a replicated microarray experiment. Biotechniques 36:324–332

    PubMed  CAS  Google Scholar 

  • Canales RD, Luo Y, Willey JC, Austermiller B, Barbacioru CC, Boysen C, Hunkapiller K, Jensen RV, Knight CR, Lee KY, Ma Y, Maqsodi B, Papallo A, Peters EH, Poulter K, Ruppel PL, Samaha RR, Shi L, Yang W, Zhang L, Goodsaid FM (2006) Evaluation of DNA microarray results with quantitative gene expression platforms. Nat Biotechnol 24:1115–1122

    Article  PubMed  CAS  Google Scholar 

  • Castro J, Bouza C, Presa P, Pino-Querido A, Riaza A, Ferreiro I, Sánchez L, Martínez P (2004) Potential sources of error in parentage assessment of turbot (Scophthalmus maximus) using microsatellite loci. Aquaculture 242:119–135

    Article  CAS  Google Scholar 

  • Cerdà J, Mercadé J, Lozano JJ, Manchado M, Tingaud-Sequeira A, Astola A, Infante C, Halm S, Viñas J, Castellana B, Asensio E, Cañavate P, Martínez-Rodríguez G, Piferrer F, Planas JV, Prat F, Yúfera M, Durany O, Subirada F, Rosell E, Maes T (2008) Genomic resources for a commercial flatfish, the Senegalese sole (Solea senegalensis): EST sequencing, oligo-microarray design, and development of the Soleamold bioinformatic platform. BMC Genomics 9:508

    Article  PubMed  CAS  Google Scholar 

  • Chadzinska M, Baginski P, Kolaczkowska E, Savelkoul HFJ, Verburg-van Kemenade BML (2008) Expression profiles of matrix metalloproteinase 9 in teleost fish provide evidence for its active role in initiation and resolution of inflammation. Immunology 125:601–610

    Article  PubMed  CAS  Google Scholar 

  • Chen J, Yang HY, Shi YH, Li MY (2009) An interaction between leukocyte cell-derived chemotaxin 2 and transferrin of ayu, Plecoglossus altivelis. Fish Shellfish Immunol 26:536–542

    Article  PubMed  CAS  Google Scholar 

  • Churchill GA (2002) Fundamentals of experimental design for cDNA microarrays. Nat Genet Suppl 32:490–495

    Article  CAS  Google Scholar 

  • Darias MJ, Zambonino-Infante JL, Hugot K, Cahu CL, Mazurais D (2008) Gene expression patterns during the larval development of European sea bass (Dicentrarchus labrax) by microarray analysis. Mar Biotechnol 10:416–428

    Article  PubMed  CAS  Google Scholar 

  • Dautremepuits C, Marcogliese DJ, Gendron AD, Fournier M (2009) Gill and head kidney antioxidant processes and innate immune system responses of yellow perch (Perca flavescens) exposed to different contaminants in the St. Lawrence River, Canada. Sci Total Environ 407:1055–1064

    Article  PubMed  CAS  Google Scholar 

  • Dobbin KK, Kawasaki ES, Petersen DW, Simon RM (2005) Characterization dye bias in microarray experiments. Bioinformatics 21:2430–2437

    Article  PubMed  CAS  Google Scholar 

  • Douglas SE, Knickle LC, Kimball J, Reith ME (2007) Comprehensive EST analysis of Atlantic halibut (Hippoglossus hippoglossus), a commercially relevant aquaculture species. BMC Genomics 8:144

    Article  PubMed  CAS  Google Scholar 

  • Draghici S, Khatri P, Eklund AC, Szallasi Z (2006) Reliability and reproducibility issues in DNA microarray measurements. Trends Genet 22:101–109

    Article  PubMed  CAS  Google Scholar 

  • Ellis AE (2001) Innate host defense mechanisms of fish against viruses and bacteria. Dev Comp Immunol 25:827–839

    Article  PubMed  CAS  Google Scholar 

  • FAO Fisheries and Aquaculture Department (2007) The state of world fisheries and aquaculture. Rome

  • Fagutao FF, Yasuike M, Caipang CM, Kondo H, Hirono I, Takahashi Y, Aoki T (2008) Gene expression profile of hemocytes of kuruma shrimp, Marsupenaeus japonicus following peptidoglycan stimulation. Mar Biotechnol 10:731–740

    Article  PubMed  CAS  Google Scholar 

  • Gjedrem T (2005) Selection and breeding programs in aquaculture. Springer, Dordrecht

    Book  Google Scholar 

  • Gjerde B, Roer JE, Lein I, Stoss J, Refstie T (1997) Heritability for body weight in farmed turbot. Aquacult Int 5:175–178

    Google Scholar 

  • Iglesias R, Paramá A, Alvarez MF, Leiro J, Fernández J, Sanmartin ML (2001) Philasterides dicentrarchi (Ciliophora, Scuticociliatida) as the causative agent of scuticociliatosis in farmed turbot Scophthalmus maximus in Galicia (NW Spain). Dis Aquat Organ 46:47–55

    Article  PubMed  CAS  Google Scholar 

  • Jenny MJ, Chapman RW, Mancia A, Chen YA, McKillen DJ, Trent H, Lang P, Escoubas JM, Bachere E, Boulo V, Liu ZJ, Gross PS, Cunningham C, Cupit PM, Tanguy A, Guo X, Moraga D, Boutet I, Huvet A, De Guise S, Almeida JS, Warr GW (2007) A cDNA microarray for Crassostrea virginica and C. gigas. Mar Biotechnol 9:577–591

    Article  PubMed  CAS  Google Scholar 

  • Jørgensen SM, Afanasyev S, Krasnov A (2008) Gene expression analyses in Atlantic salmon challenged with infectious salmon anemia virus reveal differences between individuals with early, intermediate and late mortality. BMC Genomics 9:179

    Article  PubMed  CAS  Google Scholar 

  • Jurecka P, Wiegertjes GF, Rakus KL, Pilarczyk A, Irnazarow I (2009) Genetic resistance of carp (Cyprinus carpio L.) to Trypanoplasma borreli: influence of transferrin polymorphisms. Vet Immunol Immunop 127:19–25

    Article  CAS  Google Scholar 

  • Karp NA, Lilley KS (2009) Investigating sample pooling strategies for DIGE experiments to address biological variability. Proteomics 9:388–397

    Article  PubMed  CAS  Google Scholar 

  • Kendziorski CM, Zhang Y, Lan H, Attie AD (2003) The efficiency of pooling mRNA in microarray experiments. Biostatistics 4:465–477

    Article  PubMed  CAS  Google Scholar 

  • Kendziorski C, Irizarry RA, Chen KS, Haag JD, Gould MN (2005) On the utility of pooling biological samples in microarray experiments. Proc Natl Acad Sci U S A 102:4252–4257

    Article  PubMed  CAS  Google Scholar 

  • Kim SJ, Dix DJ, Thompson KE, Murrell RN, Schmid JE, Gallagher JE, Rockett JC (2007) Effects of storage, RNA extraction, GeneChip type, and donor sex on gene expression profiling of human whole blood. Clin Chem 53:1038–1045

    Article  PubMed  CAS  Google Scholar 

  • Klebanov L, Yakovlev A (2007) How high is the level of technical noise in microarray data. Biol Direct 2:9

    Article  PubMed  CAS  Google Scholar 

  • Koski LB, Gray MW, Lang BF, Burger G (2005) AutoFACT: an automatic functional annotation and classification tool. BMC Bioinformatics 6:151

    Article  PubMed  CAS  Google Scholar 

  • Lamas J, Cepeda C, Dopazo C, Toranzo AE, Anadón R, Barja JL (1996) Occurrence of an erythrocytic virus infection in cultured turbot Scophthalmus maximus. Dis Aquat Org 24:159–167

    Article  Google Scholar 

  • Lamas J, Sanmartin ML, Parama AI, Castro R, Cabaleiro S, de Ocenda MVR, Barja JL, Leiro J (2008) Optimization of an inactivated vaccine against a scuticociliate parasite of turbot: effect of antigen, formalin and adjuvant concentration on antibody response and protection against the pathogen. Aquaculture 278:22–26

    Article  CAS  Google Scholar 

  • Lang RP, Bayne CJ, Camara MD, Cunningham C, Jenny MJ, Langdon CJ (2009) Transcriptome profiling of selectively bred Pacific oyster Crassostrea gigas families that differ in tolerance of heat shock. Mar Biotechnol. doi:10.1007/s10126-009-9181-6

    PubMed  Google Scholar 

  • Lo J, Lee S, Xu M, Liu F, Ruan H, Eun A, He Y, Ma W, Wang W, Wen Z, Peng J (2003) 15,000 unique zebrafish EST clusters and their future use in microarray for profiling gene expression patterns during embryogenesis. Genome Res 13:455–466

    Article  PubMed  Google Scholar 

  • López-Vázquez C, Dopazo CP, Olveira JG, Barja JL, Bandín I (2006) Development of a rapid, sensitive and non-lethal diagnostic assay for the detection of viral haemorrhagic septicaemia virus. J Virol Methods 133:167–174

    Article  PubMed  CAS  Google Scholar 

  • Ma Y, Lu BJ, Ruan WJ, Wang HQ, Lin J, Hu H, Deng H, Huang Q, Lai MD (2008) Tumor suppressor gene insulin-like growth factor binding protein-related protein 1 (IGFBP-rP1) induces senescence-like growth arrest in colorectal cancer cells. Exp Mol Pathol 85:141–145

    Article  PubMed  CAS  Google Scholar 

  • Magnadottir B (2006) Innate immunity of fish (overview). Fish Shellfish Immunol 20:137–151

    Article  PubMed  CAS  Google Scholar 

  • Martínez P, Hermida M, Pardo BG, Fernández C, Castro J, Cal RM, Alvarez-Dios JA, Gómez-Tato A, Bouza C (2008) Centromere-linkage in the turbot (Scophthalmus maximus) through half-tetrad analysis in diploid meiogynogenetics. Aquaculture 280:81–88

    Article  Google Scholar 

  • Martínez P, Bouza C, Hermida M, Fernández J, Toro MA, Vera M, Pardo BG, Millán A, Fernández C, Vilas R, Viñas A, Sánchez L, Felip A, Piferrer F, Cabaleiro S, Riaza A (2009) Identification of the major sex-determining region of turbot (Scophthalmus maximus). Genetics (in press)

  • Mary-Huard T, Daudin JJ, Baccini M, Biggeri A, Bar-Hen A (2007) Biases induced by pooling samples in microarray experiments. Bioinformatics 23:i313–i318

    Article  PubMed  CAS  Google Scholar 

  • Miller K, Traxler G, Kaukinen K, Li S, Richard J, Ginther N (2007) Salmonid host response to infectious hematopoietic necrosis (IHN) virus: cellular receptors, viral control, and novel pathways of defence. Aquaculture 272:S217–S237

    Article  Google Scholar 

  • Molvarec A, Prohaszka Z, Nagy B, Kalabay L, Szalay J, Fust G, Karadi I, Rigo J (2007) Association of increased serum heat shock protein 70 and C-reactive protein concentrations and decreased serum alpha(2)-HS glycoprotein concentration with the syndrome of hemolysis, elevated liver enzymes, and low platelet count. J Reprod Immunol 73:172–179

    Article  PubMed  CAS  Google Scholar 

  • Padhi A, Buchheim MA, Verghese B (2008) Dynamic evolutionary pattern of alpha(2)-macroglobulin in a model organism, the zebrafish (Danio rerio). Mol Immunol 45:3312–3318

    Article  PubMed  CAS  Google Scholar 

  • Palaksha KJ, Shin GW, Kim YR, Jung TS (2008) Evaluation of non-specific immune components from the skin mucus of olive flounder (Paralichthys olivaceus). Fish Shellfish Immunol 24:479–488

    Article  PubMed  CAS  Google Scholar 

  • Pardo BG, Fernández C, Millán A, Bouza C, Vázquez-López A, Vera M, Alvarez-Dios JA, Calaza M, Gómez-Tato A, Vázquez M, Cabaleiro S, Magariños B, Lemos ML, Leiro JM, Martínez P (2008) Expressed sequence tags (ESTs) from immune tissues of turbot (Scophthalmus maximus) challenged with pathogens. BMC Vet Res 4:37

    Article  PubMed  CAS  Google Scholar 

  • Park KC, Osborne JA, Montes A, Dios S, Nerland AH, Novoa B, Figueras A, Brown LL, Johnson SC (2009) Immunological responses of turbot (Psetta maxima) to nodavirus infection or polyriboinosinic polyribocytidylic acid (pIC) stimulation, using expressed sequence tags (ESTs) analysis and cDNA microarrays. Fish Shellfish Immunol 26:91–108

    Article  PubMed  CAS  Google Scholar 

  • Patterson TA, Lobenhofer EK, Fulmer-Smentek SB, Collins PJ, Chu TM, Bao W, Fang H, Kawasaki ES, Hager J, Tikhonova IR, Walker SJ, Zhang L, Hurban P, de Longueville F, Fuscoe JC, Tong W, Shi L, Wolfinger RD (2006) Performance comparison of one-color and two-color platforms within the MicroArray Quality Control (MAQC) project. Nat Biotechnol 24:1140–1150

    Article  PubMed  CAS  Google Scholar 

  • Peng XJ, Wood CL, Blalock EM, Chen KC, Landfield PW, Stromberg AJ (2003) Statistical implications of pooling RNA samples for microarray experiments. BMC Bioinformatics 4:26

    Article  PubMed  Google Scholar 

  • Petrik V, Saadoun S, Loosemore A, Hobbs J, Opstad KS, Sheldon J, Tarelli E, Howe FA, Bell BA, Papadopoulos MC (2008) Serum alpha(2)-HS glycoprotein predicts survival in patients with glioblastoma. Clin Chem 54:713–722

    Article  PubMed  CAS  Google Scholar 

  • Repsilber D, Ziegler A (2005) Two-color microarray experiments—technology and sources of variance. Methods Inf Med 44:400–404

    PubMed  CAS  Google Scholar 

  • Rise ML, von Schalburg KR, Brown GD, Mawer MA, Devlin RH, Kuipers N, Busby M, Beetz-Sargent M, Alberto R, Gibbs AR, Hunt P, Shukin R, Zeznik JA, Nelson C, Jones SR, Smailus DE, Jones SJ, Schein JE, Marra MA, Butterfield YS, Stott JM, Ng SH, Davidson WS, Koop BF (2004) Development and application of a salmonid EST database and cDNA microarray: data mining and interspecific hybridization characteristics. Genome Res 14:478–490

    Article  PubMed  Google Scholar 

  • Sarropoulou E, Sepulcre P, Poisa-Beiro L, Mulero V, Meseguer J, Figueras A, Novoa B, Terzoglou V, Reinhardt R, Magoulas A, Kotoulas G (2009) Profiling of infection specific mRNA transcripts of the European sea bass Dicentrarchus labrax. BMC Genomics 10:157

    Article  PubMed  CAS  Google Scholar 

  • Sato Y, Chen Z, Miyazaki K (2007) Strong suppression of tumor growth by insulin-like growth factor-binding protein-related protein 1/tumor-derived cell adhesion factor/mac25. Cancer Sci 98:1055–1063

    Article  PubMed  CAS  Google Scholar 

  • Schneider DJ, Chen Y, Sobel BE (2008) The effect of plasminogen activator inhibitor type 1 on apoptosis. Thromb Haemostasis 100:1037–1040

    CAS  Google Scholar 

  • Searle SR, Casella G, McCulloch CE (1992) Variance components. Wiley, New York

    Book  Google Scholar 

  • Secombes CJ, Wang T, Hong S, Peddie S, Crampe M, Laing KJ, Cunningham C, Zou J (2001) Cytokines and innate immunity of fish. Dev Comp Immunol 25:713–723

    Article  PubMed  CAS  Google Scholar 

  • Spruill SE, Lu J, Hardy S, Weir B (2002) Assessing sources of variability in microarray gene expression data. Biotechniques 33:916–923

    PubMed  CAS  Google Scholar 

  • Stekel D (2003) Microarray Bioinformatics. Cambridge University Press, Cambridge

    Book  Google Scholar 

  • Sun L, Zou ZY, Collodi P, Xu F, Xu XF, Zhao QS (2005) Identification and characterization of a second fibronectin gene in zebrafish. Matrix Biol 24:69–77

    PubMed  Google Scholar 

  • Toranzo AE, Barja JL (1992) First report of furunculosis in turbot reared in floating cages in North-West of Spain. Bull Eur Ass Fish Pathol 12:147–149

    Google Scholar 

  • Tsoi SCM, Ewart KV, Penny S, Melville K, Liebscher RS, Brown LL, Douglas SE (2004) Identification of immune-relevant genes from Atlantic salmon using suppression subtractive hybridization. Mar Biotechnol 6:199–214

    Article  PubMed  CAS  Google Scholar 

  • Wang HC, Zhang MH, Bianchi M, Sherry B, Sama A, Tracey KJ (1998) Fetuin (alpha(2)-HS-glycoprotein) opsonizes cationic macrophage-deactivating molecules. Proc Natl Acad Sci U S A 95:14429–14434

    Article  PubMed  CAS  Google Scholar 

  • Wang B, Li F, Luan W, Xie Y, Zhang C, Luo Z, Gui L, Yan H, Xiang J (2008) Comparison of gene expression profiles of Fenneropenaeus chinensis challenged with WSSV and Vibrio. Mar Biotechnol 10:664–675

    Article  PubMed  CAS  Google Scholar 

  • Wynne JW, O'Sullivan MG, Cook MT, Stone G, Nowak BF, Lovell DR, Elliott NG (2008) Transcriptome analyses of amoebic gill disease-affected Atlantic salmon (Salmo salar) tissues reveal localized host gene suppression. Mar Biotechnol 10:388–403

    Article  PubMed  CAS  Google Scholar 

  • Yi JK, Chang JW, Han W, Lee JW, Ko E, Kim DH, Bae JY, Yu J, Lee C, Yu MH, Noh DY (2009) Autoantibody to tumor antigen, alpha 2-HS glycoprotein: a novel biomarker of breast cancer screening and diagnosis. Cancer Epidemiol Biomarkers Prev 18:1357–1364

    Article  PubMed  CAS  Google Scholar 

  • Yoong S, O'Connell B, SoaneS A, Crowhurst MO, Lieschke GJ, Ward AC (2007) Characterization of the zebrafish matrix metalloproteinase 9 gene and its developmental expression pattern. Gene Expr Patterns 7:39–46

    Article  PubMed  CAS  Google Scholar 

  • Yue H, Eastman PS, Wang BB, Minor J, Doctolero MH, Nuttall RL, Stack R, Becker JW, Montgomery JR, Vainer M, Johnston R (2001) An evaluation of the performance of cDNA microarrays for detecting changes in global mRNA expression. Nucleic Acids Res 29:E41

    Article  PubMed  CAS  Google Scholar 

  • Zahurak M, Parmigiani G, Yu W, Scharpf RB, Berman D, Schaeffer E, Shabbeer S, Cope L (2007) Pre-processing Agilent microarray data. BMC Bioinformatics 8:142

    Article  PubMed  CAS  Google Scholar 

  • Zakharkin SO, Kim K, Mehta T, Chen L, Barnes S, Scheirer KE, Parrish RS, Allison DB, Page GP (2005) Sources of variation in Affymetrix microarray experiments. BMC Bioinformatics 6:214

    Article  PubMed  CAS  Google Scholar 

  • Zhang WY, Carriquiry A, Nettleton D, Dekkers JCM (2007) Pooling mRNA in microarray experiments and its effect on power. Bioinformatics 23:1217–1224

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

This study was supported by a Consellería de Pesca e Asuntos Marítimos and the Dirección Xeral de I+D-Xunta de Galicia project (2004/CP480) and by the Spanish Government (Consolider Ingenio Aquagenomics: CSD200700002) project. Authors wish to thank to Lucía Insua for technical assistance.

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Authors and Affiliations

  1. Departamento de Genética, Facultad de Veterinaria, Universidad de Santiago de Compostela, Campus de Lugo, 27002, Lugo, Spain

    Adrián Millán, Carlos Fernández, Belén G. Pardo, Carmen Bouza & Paulino Martínez

  2. Departamento de Matemática Aplicada, Facultad de Matemáticas, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain

    José A. Álvarez-Dios & Manuel Calaza

  3. Departamento de Geometría y Topología, Facultad de Matemáticas, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain

    Antonio Gómez-Tato

  4. Cluster de la Acuicultura de Galicia (CETGA), Punta de Couso s/n, Aguiño (Ribeira), 15965, A Coruña, Spain

    María Vázquez & Santiago Cabaleiro

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  1. Adrián Millán
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  2. Antonio Gómez-Tato
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  3. Carlos Fernández
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  4. Belén G. Pardo
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  5. José A. Álvarez-Dios
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  7. Carmen Bouza
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  9. Santiago Cabaleiro
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  10. Paulino Martínez
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Correspondence to Paulino Martínez.

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Millán, A., Gómez-Tato, A., Fernández, C. et al. Design and Performance of a Turbot (Scophthalmus maximus) Oligo-microarray Based on ESTs from Immune Tissues. Mar Biotechnol 12, 452–465 (2010). https://doi.org/10.1007/s10126-009-9231-0

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