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Gene Expression Profile of Hemocytes of Kuruma Shrimp, Marsupenaeus japonicus Following Peptidoglycan Stimulation

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Abstract

Shrimps are believed to lack an adaptive immune system and therefore rely heavily on their innate immune mechanisms to ward off pathogens. Moreover, their innate defense reactions are triggered by bacterial and fungal cell wall components such as lipopolysaccharides, peptidoglycan and β-glucans. In this study, we used microarray to examine the gene expression profile of kuruma shrimp, Marsupenaeus japonicus, after stimulation with peptidoglycan. Subsequent results show that the number of upregulated genes and percentage of differential expression (21%) was highest at day 1 poststimulation. Differentially expressed genes in day 7 and day 14, on the other hand, were 3.25% and 11.21%, respectively. Sixty-one (61) genes of unknown function were found to have responded outright to peptidoglycan (PG) stimulation. Administration of PG also caused increases in the expressions of crustin, lysozyme, and a few antibacterial peptides, all of which are known to be involved in crustacean immune response. Taken together, our results suggest that innate response in shrimp is triggered instantaneously upon exposure to a bacterial component.

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Acknowledgments

This study was supported in part by Grants-in-Aid for Scientific Researches from the Ministry of Education, Culture, Sports, Science and Technology of Japan and the grant from the Japan Science and Technology Corporation (JST); Creation and Support Program for Start-ups from Universities. Special thanks to Mr. Mudjekeewis D. Santos for proofreading the manuscript.

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Authors and Affiliations

  1. Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo, 184-8477, Japan

    Fernand F. Fagutao, Motoshige Yasuike, Christopher Marlowe Caipang, Hidehiro Kondo, Ikuo Hirono & Takashi Aoki

  2. Department of Applied Aquaculture, National Fisheries University, 2-7-1, Nagatahonmachi, Shimonoseki, Yamaguchi, Japan

    Yukinori Takahashi

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  1. Fernand F. Fagutao
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  2. Motoshige Yasuike
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  3. Christopher Marlowe Caipang
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  4. Hidehiro Kondo
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  5. Ikuo Hirono
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  6. Yukinori Takahashi
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  7. Takashi Aoki
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Correspondence to Takashi Aoki.

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Supplementary Table 1

Differentially expressed genes in each sampling period (DOC 292 KB)

Supplementary Table 2

Overview of clustered genes with their corresponding deduced functions (DOC 215 KB)

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Fagutao, F.F., Yasuike, M., Caipang, C.M. et al. Gene Expression Profile of Hemocytes of Kuruma Shrimp, Marsupenaeus japonicus Following Peptidoglycan Stimulation. Mar Biotechnol 10, 731–740 (2008). https://doi.org/10.1007/s10126-008-9110-0

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