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Thermal Sensitivity of Mitochondrial Respiration Efficiency and Protein Phosphorylation in the Clam Mercenaria mercenaria

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Abstract

The mitochondria of intertidal invertebrates continue to function when organisms are exposed to rapid substantial shifts in temperature. To test if mitochondrial physiology of the clam Mercenaria mercenaria is compromised under elevated temperatures, we measured mitochondrial respiration efficiency at 15°C, 18°C, and 21°C using a novel, high-throughput, microplate respirometry methodology developed for this study. Though phosphorylating (state 3) and resting (state 4) respiration rates were unaffected over this temperature range, respiratory control ratios (RCRs: ratio of state 3 to state 4 respiration rates) decreased significantly above 18°C (p < 0.05). The drop in RCR was not associated with reduction of phosphorylation efficiency, suggesting that, while aerobic scope of mitochondrial respiration is limited at elevated temperatures, mitochondria continue to efficiently produce adenosine triphosphate. We further investigated the response of clam mitochondria to elevated temperatures by monitoring phosphorylation of mitochondrial protein. Three proteins clearly demonstrated significant time- and temperature-specific phosphorylation patterns. The protein-specific patterns of phosphorylation may suggest that a suite of protein kinases and phosphatases regulate mitochondrial physiology in response to temperature. Thus, while aerobic scope of clam mitochondrial respiration is reduced at moderate temperatures, specific protein phosphorylation responses reflect large shifts in function that are initiated within the organelle at higher temperatures.

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Acknowledgments

We are grateful to J. Ewart for providing the clams for these experiments. We would like to acknowledge Dr. T. Hanson for his assistance with the protein phosphorylation experiments. This work was supported by SeaGrant R/B-43 and NSF grant #0238281.

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  1. College of Marine and Earth Studies, University Delaware, 700 Pilottown Road, Lewes, DE, 19958, USA

    P. N. Ulrich & A. G. Marsh

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  1. P. N. Ulrich
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  2. A. G. Marsh
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Correspondence to A. G. Marsh.

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Ulrich, P.N., Marsh, A.G. Thermal Sensitivity of Mitochondrial Respiration Efficiency and Protein Phosphorylation in the Clam Mercenaria mercenaria . Mar Biotechnol 11, 608–618 (2009). https://doi.org/10.1007/s10126-009-9177-2

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  • DOI: https://doi.org/10.1007/s10126-009-9177-2

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