Abstract
Both coccolith-bearing cells (C-cells) and naked cells (N-cells) of the coccolithophorid Pleurochrysis haptonemofera can grow in salinities of more than 7‰ (about 20% of a “normal” sea water salinity [35‰]), with the highest growth rates in salinities of more than 14‰. Microscopic observations of cells suspended in 100 mM NaCl (7‰) showed that, while N-cells were swelling uniformly all over the cell surface, C-cells were bulging the plasma membrane from the hole of the coccosphere at the apical (flagellar) pole of the cell. Effects of several cations and anions on the morphological change of C-cells under hypoosmotic pressure were investigated. When 100 mM K+ was used, protoplasts were released from the coccosphere completely in almost all the cells. This phenomenon was shown with K+ most effectively. The protoplasts could grow in the fresh medium and form the first coccolith within 9 h.
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Acknowledgments
The authors thank Drs. Isao Inouye and Masanobu Kawachi of Tsukuba University, Japan, for providing the P. haptonemofera cells, and Ms. Tamiko Minamisawa of Tokyo University of Pharmacy and Life Science, Japan, for helpful comments. We are indebted to Dr. Shigeyuki Kawano, Graduate School of Frontier Sciences, the University of Tokyo for his kind help with electron microscopy. This work was supported by Grants-in-Aid from the Ministry of Education, Science, Sports and Culture, Japan (13640657, 13740463, and 13874112), the Promotion and Mutual Aid Corporation for Private Schools, and a Sasakawa Scientific Research Grant from The Japan Science Society to Y. H.
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Takayanagi, T., Hirokawa, Y., Yamamoto, M. et al. Protoplast Formation of the Coccolithophorid Pleurochrysis haptonemofera in Hypoosmotic K+ Solution: Shedding of the Coccosphere and Regrowth of the Protoplast in Normal Medium. Mar Biotechnol 9, 56–65 (2007). https://doi.org/10.1007/s10126-006-6058-9
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DOI: https://doi.org/10.1007/s10126-006-6058-9