Skip to main content

Advertisement

SpringerLink
Log in

Structural Analysis of cDNAs Coding for 4SNc-Tudor Domain Protein from Fish and Their Expression in Yellowtail Organs

  • Published:
Marine Biotechnology Aims and scope Submit manuscript

Abstract

We cloned complementary DNAs for 4SNc-Tudor protein (SN4TDR) from yellowtail (Seriola quinqueradiata), torafugu (Takifugu rubripes), and zebrafish (Danio rerio). This protein contains 4 staphylococcal nuclease domains at the N terminus followed by a Tudor domain. We also identified the 4SNc-Tudor proteins highly homologous to that in yellowtail from the Takifugu genomic database. According to the smart database, these fish proteins had an overlapping Tudor domain (smart00333) with a complete 5 SNc domain (smart00318). In addition, 2 possible translation start sites were observed at the 5′ sequences in all 3 fish species. Northern blot analysis of different yellowtail organs showed that the full SN4TDR messenger RNA was approximately 4000 nucleotides long and that its expression was highest in liver and gallbladder, being about 2 to 5 times higher than in kidney, brain, ovary, and gills, and exceedingly low in spleen, heart, and muscle. A minor 2000-nucleotide transcript observed in kidney, spleen, and gallbladder, was attributable to an alternatively spliced variant of this gene. Total proteins extracted from yellowtail liver were fractionated by heparin affinity column chromatography and separated by sodium dodecylsulfate polyacrylamide gel electrophoresis. Analyses by SDS-PAGE and liquid chromatography with tandem mass spectroscopy identified the polypeptide encoded by SN4TDR as a single molecule of 102 kDa.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Abe S, Sakai M, Yagi K, Hagino T, Ochi K, Shibata K, Davies E (2003) A Tudor protein with multiple SNc domains from pea seedlings: cellular localization, partial characterization, sequence analysis, and phylogenetic relationships. J Exp Bot 54: 971–983

    Article  Google Scholar 

  • Abe S, Chamnan C, Miyamoto K, Minamino Y, Nouda M (2004a) Isolation and identification of 3-methylcrotonyl coenzyme A carboxylase cDNAs and pyruvate carboxylase, and their expression in red seabream (Pagrus major) organs. Mar Biotechnol 6, 527–540

    Article  Google Scholar 

  • Abe S, Chiba S, Mishra N, Minamino Y, Nakasuji H, Doi M, Gray TA (2004) Origin and evolution of the genomic region encoding RAF1, MKRN2, PPARG, and SYN2 in human chromosome 3p25. Mar Biotechnol 6: S404–S412

    Article  Google Scholar 

  • Abe S, Davies E (1991) Isolation of F-actin from pea stems: Evidence from fluorescence microscopy. Protoplasma 163: 51–61

    Article  Google Scholar 

  • Broadhurst MK, Wheeler TT (2001) The p100 coactivator is present in the nuclei of mammary epithelial cells and its abundance is increased in response to prolactin in culture and in mammary tissue during lactation. Journal of Endocrinology 171: 329–337

    Article  Google Scholar 

  • Caudy AA, Ketting RF, Hammond SM, Denli AM, Bathoorn AM, Tops BB, Silva JM, Myers MM, Hannon GJ, Plasterk RH (2003) A micrococcal nuclease homologue in RNAi effector complexes. Nature 425(6956): 411–414

    Article  Google Scholar 

  • Chamnan C, Abe S, Doi M, Chiba S, Gray TA (2003) The genomic organization of MKRN1, and expression profile of MKRN1, MKRN2, and RAF1 in yellowtail fish (Seriola quinqueradiata). Histol Histochem 42: 57–75

    Google Scholar 

  • Davies E, Stankovic B, Azama K, Shibata K, Abe S (2001) Novel components of the plant cytoskeleton: A beginning to plant ‘cytomics’. Plant Science 160: 185–196

    Article  Google Scholar 

  • Ito Y, Abe S, Davies E (1994) Co-localization of cytoskeleton proteins and polysomes with a membrane fraction from peas. Journal of Experimental Botany 45: 253–359

    Google Scholar 

  • Gray TA, Azama K, Whitmore K, Min A, Abe S, Nicholls RD (2001) Phylogenetic conservation of the makorin-2 gene, encoding multiple zinc-finger protein,antisense to the RAF1 proto-oncogene. Genomics 77: 119–126

    Article  Google Scholar 

  • Keefe LJ, Sondek J, Shortle D, Lattman EE (1993) The alpha aneurism: a structural motif revealed in an insertion mutant of staphylococcal nuclease. Proceedings of the National Academy of Sciences of the United States of America 90: 3275–3279

    Google Scholar 

  • Keefe LJ, Quirk S, Gittis A, Sondek J, Lattman EE (1994) Accommodation of insertion mutations on the surface and in the interior of staphylococcal nuclease. Protein Science 3: 391–401

    Google Scholar 

  • Kozak M (1999) Initiation of translation in prokaryotes and eukaryotes. Gene 2: 187–208

    Google Scholar 

  • Liu Z, Kim S, Karsi A (2001) Channel catfish follicle-stimulating hormone and luteinizing hormone: Complementary DNA cloning and expression during ovulation. Mar Biotechnol 3: 590–599

    Article  Google Scholar 

  • Peyush P, Moriyama S, Takahashi A, Kawauchi H (2000) Molecular cloning of growth hormone complementary DNA in barfin flounder (Verasper moseri). Mar Biotechnol 2: 21–26

    Google Scholar 

  • Pohar N, Godenschwege TA, Buchner E (1999) Invertebrate tissue inhibitor of metalloproteinase: Structure and nested gene organization within the synapsin locus is conserved from Drosophila to human. Genomics 57(2): 293–296

    Article  Google Scholar 

  • Ponting CP (1997) Tudor domains in proteins that interact with RNA. Trends Biochem Sci 2: 51–52

    Google Scholar 

  • Porta A, Colonna-Romano S, Callebaut I, Franco A, Marzullo L, Kobayashi GS, Maresca B (1999) An homologue of the human 100-kDa protein (p100) is differentially expressed by Histoplasma capsulatum during infection of murine macrophages. Biochem Biophys Res Commun 254: 605–613

    Article  Google Scholar 

  • Sami-Subbu R, Choi S-B, Wu Y, Wang C, Okita TW (2001) Identification of a cytoskeleton-associated 120 kDa RNA-binding protein in developing rice seeds. Plant Mol Biol 46: 79–88

    Article  Google Scholar 

  • Shibata K, Morita Y, Abe S, Stankovic B, Davies E (1999) Apyrase from pea stems: Isolation, purification, characterization and identification of a NTPase from the cytoskeleton fraction of pea stem tissue. Plant Physiol Biochemistry 37: 881–888

    Article  Google Scholar 

  • Strausberg RL, Feingold EA, Marra MA (2002) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. J Proc Natl Acad Sci USA 99(26): 16899–16903

    Google Scholar 

  • Tijsterman M, May RC, Simmer F, Okihara KL, Plasterk RH (2004). Genes required for systemic RNA interference in Caenorhabditis elegans. Current Biology 14(2): 111–116

    Article  Google Scholar 

  • Tong X, Drapkin R, Yalamanchili R, Mosialos G, Kieff E (1995) The Epstein-Barr virus nuclear protein 2 acidic domain forms a complex with a novel cellular coactivator that can interact with TFIIE. Mol Cell Biol 15: 4735–4744

    Google Scholar 

  • Wang JR, Li XL, Fan SQ, Tan C, Xiang JJ, Tang K, Wang R, Li GY (2003) Expression of LRRC4 has the potential to decrease the growth rate and tumorigenesis of glioblastoma cell line U251. Ai Zheng. 22(9): 897–902

    Google Scholar 

  • Yang J, Aittomäki S, Pesu M, Carter K, Saarinen J, Kalkkinen N, Kieff E, Silvennoinen O (2002) Identification of p100 as a coactivator for STAT6 that bridges STAT6 with RNA polymerase II. EMBO J 21(18): 4950–4958

    Article  Google Scholar 

  • Zhao CT, Shi KH, Su Y, Liang LY, Yan Y, Postlethwait J Meng AM (2003) Two variants of zebrafish p100 are expressed during embryogenesis and regulated by Nodal signaling. FEBS Lett 543(1–3): 190–195

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Molecular Cell Biology, Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, 790-8566, Japan

    Shunnosuke Abe, Pi-Lin Wang, Fuminori Takahashi & Eiji Sasaki

Authors
  1. Shunnosuke Abe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pi-Lin Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Fuminori Takahashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Eiji Sasaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shunnosuke Abe.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Abe, S., Wang, PL., Takahashi, F. et al. Structural Analysis of cDNAs Coding for 4SNc-Tudor Domain Protein from Fish and Their Expression in Yellowtail Organs. Mar Biotechnol 7, 677–686 (2005). https://doi.org/10.1007/s10126-004-5137-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10126-004-5137-z

Keywords

Search

Navigation