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Sexually dimorphic expression and regulatory sequence of dnali1 in the olive flounder Paralichthys olivaceus

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Abstract

Dynein axonemal light intermediate chain 1 (dnali1) is an important part of axonemal dyneins and plays an important role in the growth and development of animals. However, there is little information about dnali1 in fish. Herein, we cloned dnali1 gene from the genome of olive flounder (Paralichthys olivaceus), a commercially important maricultured fish in China, Japan, and Korea, and analyzed its expression patterns in different gender fish. The flounder dnali1 DNA sequence contained a 771 bp open reading frame (ORF), two different sizes of 5′ untranslated region (5′UTR), and a 1499 bp 3′ untranslated region (3′UTR). Two duplicated 922 nt fragments were found in dnali1 mRNA. The first fragment contained the downstream coding region and the front portion of 3′UTR, and the second fragment was entirely located in 3′UTR. Multiple alignments indicated that the flounder Dnali1 protein contained the putative conserved coiled-coil domain. Its expression showed sexually dimorphic with predominant expression in the flounder testis, and lower expression in other tissues. The gene with the longer 5′UTR was specifically expressed in the testis. The highest expression level in the testis was detected at stages IV and V. Transient expression analysis showed that the 922 bp repeated sequence 3′UTR of dnali1 down-regulated the expression of GFP at the early stage in zebrafish. The flounder dnali1 might play an important role in the testis, especially in the period of spermatogenesis, and the 5′UTR and the repetitive sequences in 3′UTR might contain some regulatory elements for the cilia.

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Abbreviations

dnali1 :

Dynein axonemal light intermediate chain 1

ORF:

Open reading frame

5′UTR:

5′ Untranslated region

3′UTR:

3′ Untranslated region

TL:

Total length; MS222, tricaine methane sulfonate

HE:

Hematoxylin/eosin

RT-PCR:

Reverse transcription polymerase chain reaction

RACE:

Rapid amplification of cDNA ends

qPCR:

Real-time quantitative polymerase chain reaction

IDA:

Inner dynein arms

ODA:

Outer dynein arms

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Acknowledgements

This work was supported by the projects from the National Sciences of Foundation of China (Nos. 31772834, 31672636, and 31702337), and the National Key R&D Program of China (No. 2018YFD0900202) and Key Technology Research and Development Program of Shandong (CN) (No. 2018YFD0901202).

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Authors and Affiliations

  1. CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, P.R. China

    Ling Wang, Xungang Tan, Congcong Zou, Lijuan Wang, Zhihao Wu, Yuxia Zou & Feng You

  2. Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, 266071, P.R. China

    Ling Wang, Xungang Tan, Congcong Zou, Lijuan Wang, Zhihao Wu, Yuxia Zou & Feng You

  3. University of Chinese Academy of Sciences, Beijing, 10049, P.R. China

    Ling Wang & Congcong Zou

  4. Shenghang Aquatic Science and Technology Co. Ltd, Weihai, China

    Zongcheng Song

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  1. Ling Wang
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11033_2021_6342_MOESM2_ESM.tif

Developmental stages of the flounder gonads. The stages were determined using histological section with HE staining (tif 21396 kb)

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Wang, L., Tan, X., Zou, C. et al. Sexually dimorphic expression and regulatory sequence of dnali1 in the olive flounder Paralichthys olivaceus. Mol Biol Rep 48, 3529–3540 (2021). https://doi.org/10.1007/s11033-021-06342-9

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