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The hypervariable domain of the mitochondrial control region in Atlantic spiny lobsters and its potential as a marker for investigating phylogeographic structuring

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Abstract

Atlantic spiny lobsters support major fisheries in northeastern Brazilian waters and in the Caribbean Sea. To avoid reduction in diversity and elimination of distinct stocks, understanding their population dynamics, including structuring of populations and genetic diversity, is critical. We here explore the potential of using the hypervariable domain in the control region of the mitochondrial DNA as a genetic marker to characterize population subdivision in spiny lobsters, using Panulirus argus as the species model. The primers designed on the neighboring conserved genes have amplified the entire control region (approx. 780 bases) of P. argus and other closely related species. Average nucleotide and haplotype diversity within P. argus were found to be high, and population structuring was hypothesized. The data suggest a division of P. argus into genetically different phylogeographic groups. The hypervariable domain seems to be useful for determining genetic differentiation of geographically distinct stocks of P. argus and other Atlantic spiny lobsters.

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Acknowledgments

The authors are grateful to the numerous people who helped in this study by “sharing” lobster tissue samples: Dr. Mark Butler IV and Dr. Don Behringer (Old Dominion University, Norfolk, Virginia, U.S.A.); Dr. Michael J. Childress and Dr. Margaret B. Ptacek (Clemson University, Clemson, South Carolina, U.S.A. [Lobster Phylogeny Project]); Fred Lentz (Borinquen Aquaculture) and Jose A. Rivera (NOAA) from Puerto Rico; James Azueta and Ramon Carcamo, Jr. (Belize Fisheries Department); Nicole Esteban (St. Eustatius National and Marine Parks) from the Netherlands Antilles); Rui M. Freitas (Universidade do Algarve, Faro, Portugal); B. Chakalall (FAO, Subregional Office for the Caribbean); Italo Vieira (IBAMA/CEPNOR, Para, Brazil); and Lim Jeung Sik (Napesca, Espírito Santo, Brazil). Useful comments from 2 anonymous reviewers are gratefully acknowledged. F.M. Diniz is supported by a PhD scholarship from the Brazilian Government (CAPES Scholarship Program).

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Authors and Affiliations

  1. Biomedical Sciences Building, School of Biological Sciences, University of Southampton, Bassett Crescent East, Southampton, SO16 7PX, U.K.

    Fabio M. Diniz & Norman Maclean

  2. CP:01, CEP:64., EMBRAPA Meio-Norte, 006-220, Teresina, PI, Brazil

    Fabio M. Diniz

  3. Department of Fisheries Engineering, Federal University of Ceará, Fortaleza, Brazil

    Masayoshi Ogawa

  4. Department of Aquatic Sciences, Federal Rural University of Amazônia, Belém, Brazil

    Israel H.A. Cintra

  5. Department of Biology, Dalhousie University, Halifax, N.S., Canada

    Paul Bentzen

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  1. Fabio M. Diniz
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  2. Norman Maclean
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  3. Masayoshi Ogawa
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Correspondence to Fabio M. Diniz.

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Diniz, F.M., Maclean, N., Ogawa, M. et al. The hypervariable domain of the mitochondrial control region in Atlantic spiny lobsters and its potential as a marker for investigating phylogeographic structuring. Mar Biotechnol 7, 462–473 (2005). https://doi.org/10.1007/s10126-004-4062-5

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