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Cloning and Characterization of Microsatellite Loci in a Gorgonian Coral, Junceella juncea (Anthozoa; Octocorallia; Ellisellidae) and Its Application in Clonal Genotyping

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Abstract

We attempted to isolate microsatellites from a Symbiodinium-free gorgonian octocoral, Junceella juncea, using two methods, partial genomic library screening and enrichment. Among the 3856 clones screened by the partial library method, 10 possibly positive signals were found, and 3 of them could be used to design primers and amplified consistently. In contrast, only one locus isolated by the enrichment method gave reliable amplification and was useful. The results indicate that microsatellites are rare in Junceella juncea, as reported for other cnidarians. Overall, we obtained 4 polymorphic loci to test the feasibility in investigating clonal structure of J. juncea. A total of 40 multilocus genotypes were found among 152 colonies, and the number of genotypes (clones) identified at 7 reefs ranged from 2 to 16. The results of a nonmetric multidimensional scaling analysis indicated the recruitment of J. juncea populations mainly comes from self-retention. These novel microsatellite loci will provide a useful tool to study clonal structure and population genetics for J. juncea in the future.

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Acknowledgments

Allen Chen offered discussions and comments on an earlier draft. Many people in H.-T. Yu’s lab shared their experiences in cloning. We thank them all. This study was supported by the National Science Council of Taiwan (NSC90-2311-B-291-003).

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Authors and Affiliations

  1. Institute of Oceanography, National Taiwan University, Taipei, Taiwan, ROC 10617

    Shang-Yin Vanson Liu & Chang-Feng Dai

  2. National Museum of Marine Biology and Aquarium, Pingtung, Taiwan, ROC 944

    Tung-Yung Fan

  3. Institute of Zoology and Department of Life Science, National Taiwan University, Taipei, Taiwan, ROC 10617

    Hon-Tsen Yu

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  1. Shang-Yin Vanson Liu
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  2. Chang-Feng Dai
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  3. Tung-Yung Fan
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  4. Hon-Tsen Yu
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Correspondence to Hon-Tsen Yu.

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Liu, SY.V., Dai, CF., Fan, TY. et al. Cloning and Characterization of Microsatellite Loci in a Gorgonian Coral, Junceella juncea (Anthozoa; Octocorallia; Ellisellidae) and Its Application in Clonal Genotyping. Mar Biotechnol 7, 26–32 (2005). https://doi.org/10.1007/s10126-004-0015-2

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  • DOI: https://doi.org/10.1007/s10126-004-0015-2

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