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Isolation and Characterization of Nine Microsatellite Loci from the Hawaiian Grouper Epinephelus Quernus (Serranidae) for Population Genetic Analyses

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Abstract

The availability of variable genetic markers for groupers (Serranidae) has generally been limited to mitochondrial DNA. For studies of population genetic structure, more loci are usually required; particularly useful are those that are nuclear in origin such as microsatellites. Here, we isolated and characterized 9 microsatellite loci from the endemic Hawaiian grouper Epinephelus quernus using a biotin-labeled oligonucleotide-streptavidin–coated magnetic bead approach. Of the 20 repeat-containing fragments isolated, 15 had sufficient flanking region in which to design primers. Among these, 9 produced consistent polymerase chain reaction product, and 6 were highly variable. These 6 loci were all composed of dinucleotide repeats, with the number of alleles ranging from 6 to 18, and heterozygosities from 33.3% to 91.7%. The high levels of variability observed should make these markers useful for population genetic studies of E. quernus, and potentially other epinephelines.

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Acknowledgements

We thank Corinna Lange from the Centre for Identification and Diagnostics, University of Queensland, for her help and support in microsatellite library development. We also thank the Hawaii Institute of Marine Biology, Christopher Kelley, Virginia Moriwake, Malia Chow, Aaron Moriwake, Bo Alexander, E.G. Grau, Hal Richman, and the many bottomfish fishermen of Hawaii who collected samples for this work. Funding was provided by the University of California, Berkeley, to G.K. Roderick, and travel expenses to the University of Queensland were provided in part by the Haumana Biomedical Program administered through the University of Hawaii.

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Authors and Affiliations

  1. Department of Environmental Science, Policy and Management, University of California, Berkeley, 201 Wellman Hall #3112, Berkeley, CA 94720-3112, USA

    Malia Ana J. Rivera & George K. Roderick

  2. School of Life Sciences, The University of Queensland, St Lucia, Australia

    Glenn C. Graham

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  1. Malia Ana J. Rivera
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  2. Glenn C. Graham
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  3. George K. Roderick
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Correspondence to Malia Ana J. Rivera.

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Rivera, M.J., Graham, G.C. & Roderick, G.K. Isolation and Characterization of Nine Microsatellite Loci from the Hawaiian Grouper Epinephelus Quernus (Serranidae) for Population Genetic Analyses . Mar. Biotechnol. 5, 126–129 (2003). https://doi.org/10.1007/s10126-002-0093-y

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  • DOI: https://doi.org/10.1007/s10126-002-0093-y

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