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High Frequency and Large Number of Polymorphic Microsatellites in Cultured Shrimp, Penaeus (Litopenaeus) vannamei [Crustacea:Decapoda]

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Abstract

A total of 1479 recombinant clones were obtained from a Sau3A-digested genomic library of Penaeus (Litopenaeus) vannamei and used for probe hybridization. Of the 251 clones that tested positive to one or more of the probes and were sequenced, 173 (69%) contained 573 simple sequence repeats, or microsatellites, with 3 or more repeats. The frequency of microsatellites with 3, 5, and 10 or more repeats was 1 in 0.94 kb, 1 in 2.78 kb, and 1 in 5.94 kb, respectively. To increase the number of polymorphic markers for mapping, 136 primer sets that flanked microsatellites containing single or multiple motifs with 3 or more repeats were designed and tested. Of the 136 primers, 93 (68.0%) were polymorphic in cultured shrimp, with polymorphism information content (PIC) values ranging from 0.195 to 0.873, and observed heterozygosities ranging from 10% to 100%. These markers are being used along with other markers to construct a linkage map for P. vannamei.

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Acknowledgements

Thanks to Steve Arce, Brad Argue, Fernanda Calderon, William Carr, Shaun Moss, Monica Traub, and Jim Sweeney, from the Oceanic Institute, Honolulu, Hawaii, for access to shrimp tissue samples, Kelly Johnson for assistance with plasmid DNA isolation, and Kireina Bell for editing the cloned sequences. This work was supported by the U.S. Department of Agriculture, through a grant (#95-388-1424) to the USMSFP. Partial support was received from the Department of Environmental and Population Health at Tufts University School of Veterinary Medicine.

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  1. Environmental and Comparative Genomics Section, Department of Environmental and Population Health, Tufts University School of Veterinary Medicine, 200 Westboro Road, North Grafton, MA 01536, USA

    Dawn Meehan, Zhenkang Xu, Gladys Zuniga & Acacia Alcivar-Warren

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  1. Dawn Meehan
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  2. Zhenkang Xu
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Correspondence to Acacia Alcivar-Warren.

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Meehan, D., Xu, Z., Zuniga, G. et al. High Frequency and Large Number of Polymorphic Microsatellites in Cultured Shrimp, Penaeus (Litopenaeus) vannamei [Crustacea:Decapoda] . Mar. Biotechnol. 5, 311–330 (2003). https://doi.org/10.1007/s10126-002-0092-z

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