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Filamentous Marine Fungi as Producers of O-Glycosylhydrolases: β-1,3-Glucanase from Chaetomium indicum

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Abstract

Ninety fungal strains (42 species) isolated from marine habitats were studied for their ability to produce extracellular enzymes. Cultural filtrates of these strains were shown to contain a series of glycosidases (β-glucosidases, N-acetyl-β-glucosaminidases, β-galactosidases α-mannosidases) and glucanases (1,3-β-glucanases, amylases) which varied with habitat. The level of activity depended on the species of fungi. Several promising strains capable of producing both individual enzymes and a set of enzymes for splitting carbohydrate-containing compound have been isolated. Optimal conditions for growth of Chaetomium indicum and for biosynthesis of β-1,3-glucanase were determined. β-1,3-Glucanase was isolated using ion-exchange chromatography, ultrafiltration, and gel filtration. The presence of 2 enzyme forms was shown; both forms were exo-β-1,3-glucanases.

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Acknowledgements

We are grateful to Mrs. N. Shepetova for helpful discussions and translating this article into English. This work was supported by the Governmental Program “Intergratsiya” grant of the Russian Foundation of Fundamental Research 02-04-49518, and grant 01-04-06197, and by grant Rec-003 of the U.S. Civilian Research & Development Foundation for the Independent States of the Former Soviet Union (CRDF) and Ministry of Education of the Russian Federation.

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  1. Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, 690022, Vladivostok-22, Russia

    Yu. V. Burtseva, N. S. Verigina, V. V. Sova, M. V. Pivkin & T. N. Zvyagintseva

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  1. Yu. V. Burtseva
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  2. N. S. Verigina
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  3. V. V. Sova
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  4. M. V. Pivkin
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  5. T. N. Zvyagintseva
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Correspondence to M. V. Pivkin.

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Burtseva, Y., Verigina, N., Sova, V. et al. Filamentous Marine Fungi as Producers of O-Glycosylhydrolases: β-1,3-Glucanase from Chaetomium indicum . Mar. Biotechnol. 5, 349–359 (2003). https://doi.org/10.1007/s10126-002-0070-2

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  • DOI: https://doi.org/10.1007/s10126-002-0070-2

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