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Genomic Cloning of Mud Loach Misgurnus mizolepis (Cypriniformes, Cobitidae) β-Actin Gene and Usefulness of Its Promoter Region for Fish Transgenesis

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Abstract

As a part of our effort to obtain a strong regulatory element for the construction of an autogenic mud loach transgenic vector, we isolated a genomic clone that contains an open reading frame encoding the β-actin gene, then examined the transcriptional activity of the upstream sequences, including the first intron, in transiently transfected cell lines. It showed that the upstream region has substantially strong transcriptional activity, and that both the proximal promoter and distal region of intron 1 play a crucial role in the activity. A similar result, based on fish growth, was obtained with the expression vectors containing the growth hormone gene of mud loach. These were driven by the regulatory region of the mud loach β-actin gene with various mutations, and were directly transferred into the trunk muscle of fish using an electrostimulation-mediated method. Fish weights were monitored over the next 4 weeks. These data suggest that the proximal promoter and the first intron enhancer of the mud loach β-actin gene are useful for autogenic mud loach transgenesis.

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References

  1. M.S. Alam F.L. Lavender A. lyengar M.A. Rahman H.H. Ayad R. Lathe S.D. Morley N. Maclean (1996) ArticleTitleComparison of the activity of carp and rat β-actin gene regulatory sequences in tilapia nd rainbow trout embryos. Mol Reprod Dev 45 117–122 Occurrence Handle10.1002/(SICI)1098-2795(199610)45:2<117::AID-MRD2>3.3.CO;2-7 Occurrence Handle1:CAS:528:DyaK28XmsFGmtLw%3D Occurrence Handle8914067

    Article  CAS  PubMed  Google Scholar 

  2. W.D. Benton R.W. Davis (1977) ArticleTitleScreening λ, gt recombinant clones by hybridization to a single plaque in situ. Science 196 180–182 Occurrence Handle1:STN:280:CSiC2M%2FnsF0%3D Occurrence Handle322279

    CAS  PubMed  Google Scholar 

  3. J.H. Chae Y.H. Lee C.G. Kim (1999) ArticleTitleTranscription factor CP2 is crucial in hemoglobin synthesis during erythroid terminal differentiation in vitro. Biochem Biophys Res Commun 263 580–583 Occurrence Handle10.1006/bbrc.1999.1408 Occurrence Handle1:CAS:528:DyaK1MXlvFams74%3D Occurrence Handle10491335

    Article  CAS  PubMed  Google Scholar 

  4. S.J. Du Z. Gong C.L. Hew C.H. Tan G.L. Fletcher (1992) ArticleTitleDevelopment of an all-fish gene cassette for gene transfer in aquaculture. Mol Mar Biol Biotechnol 1 290–300 Occurrence Handle1:CAS:528:DyaK3sXhvVCmt7k%3D Occurrence Handle1308820

    CAS  PubMed  Google Scholar 

  5. R.M. Frederickson M.R. Micheau A. Iwamoto N.G. Miyamoto (1989) ArticleTitle5′ Flanking and first intron sequences of the human β-actin gene required for efficient promoter activity. Nucleic Acids Res 17 253–270 Occurrence Handle1:CAS:528:DyaL1MXht12lu7s%3D Occurrence Handle2911466

    CAS  PubMed  Google Scholar 

  6. Z. Gong M.J. King G.L. Fletcher C.L. Hew (1995) ArticleTitleThe antifreeze protein genes of the winter flounder, Pleuronectus americanus, are differentially regulated in liver and non-liver tissues. Biochem Biophys Res Commun 206 387–392 Occurrence Handle10.1006/bbrc.1995.1053 Occurrence Handle1:CAS:528:DyaK2MXjtVWlsL4%3D Occurrence Handle7818543

    Article  CAS  PubMed  Google Scholar 

  7. F.L. Graham A.J. van der Eb (1973) ArticleTitleA new technique for the assay of infectivity of human adenovirus 5. Virology 52 456–467 Occurrence Handle1:STN:280:CSyC1c%2Fot10%3D Occurrence Handle4705382

    CAS  PubMed  Google Scholar 

  8. L.E. Hightower J.L. Renfro (1988) ArticleTitleRecent applications of fish cell culture to biomedical research. J Exp Zool 248 290–302 Occurrence Handle1:STN:280:BiaD1MvislY%3D Occurrence Handle3062124

    CAS  PubMed  Google Scholar 

  9. S. Irniger C.M. Egli M. Kuenzler G.H. Braus (1992) ArticleTitleThe yeast actin intron contains a cryptic promoter that can be switched on by preventing transcriptional interference. Nucleic Acids Res 20 4733–4739 Occurrence Handle1:CAS:528:DyaK38Xmt1Olurw%3D Occurrence Handle1408785

    CAS  PubMed  Google Scholar 

  10. J.M. Jankowski G.H. Dixon (1984) ArticleTitleTranscription of a trout protamine gene in vitro: the effects of alteration of promoters. Can J Biochem Cell Biol 62 291–300 Occurrence Handle1:CAS:528:DyaL2cXksV2nt7g%3D Occurrence Handle6329491

    CAS  PubMed  Google Scholar 

  11. G.M. Kelly B. Reversade (1997) ArticleTitleCharacterization of a cDNA encoding a novel band 4.1-like protein in zebrafish. Biochem Cell Biol 75 623–632 Occurrence Handle1:CAS:528:DyaK1cXitFCiurc%3D Occurrence Handle9551184

    CAS  PubMed  Google Scholar 

  12. C.G. Kim (1996) ArticleTitleA simple embryonic stem cell-based in vitro differentiation system that recapitulates early erythropoietic event in mouse embryo. Korean J Zool 39 239–247

    Google Scholar 

  13. D.S. Kim Y.K. Nam J.Y. Jo (1997) ArticleTitleEffect of oestradiol-17β17 immersion treatment of sex reversal of mud loach, Misgurnus mizolepis (funther). Aquacult Res 28 941–946

    Google Scholar 

  14. N.-G. Kim J.H. Chae S.Y. Choe C.G. Kim (2000) ArticleTitleReporter constructs showing proper globin gene switching in cultured erythroid cell lines. Mol Cells 10 281–288 Occurrence Handle1:CAS:528:DC%2BD3cXks1Kkt7g%3D Occurrence Handle10901166

    CAS  PubMed  Google Scholar 

  15. J.-S. Lee (2000) ArticleTitleThe internally self-fertilizing hermaphroditic teleost Rivulus marmoratus (Cyprinodontiformes, Rivulidae) β-actin gene: amplification and sequence analysis with conserved primers. Mar Biotechnol 2 161–166 Occurrence Handle1:CAS:528:DC%2BD3cXjtF2jtrg%3D Occurrence Handle10811955

    CAS  PubMed  Google Scholar 

  16. J.-S. Lee T.M. Franklin J.K. Chipman (2000a) ArticleTitleCloning of the β-actin gene from the European flounder (Platichthys flesus L.). DNA Seq 11 83–86 Occurrence Handle1:CAS:528:DC%2BD3MXhtVGru7o%3D

    CAS  Google Scholar 

  17. J.-S. Lee S.-H. Lee M.C. Gye (2000b) ArticleTitleThe β-actin gene of two species of southern top mouth minnow (Pseudorasbora parva) and the common fat minnow (Rhynchocypris oxycephalus) from the family Cyprinidae. DNA Seq 11 301–307

    Google Scholar 

  18. Z.J. Liu B. Moav A.J. Faras K.S. Guise A.R. Kapuscinski P.B. Hackett (1990a) ArticleTitleFunctional analysis of elements affecting expression of the β-actin gene of carp. Mol Cell Biol 10 3432–3440 Occurrence Handle1:CAS:528:DyaK3cXksFemtbk%3D

    CAS  Google Scholar 

  19. Z.J. Liu Z.Y. Zhu K. Roberg A. Faras K. Guise A.R. Kapuscinski P.B. Hackett (1990b) ArticleTitleIsolation and characterization of beta-actin gene of carp (Cyprinus carpio). DNA Seq 1 125–136 Occurrence Handle1:CAS:528:DyaK3MXlvF2qsbo%3D

    CAS  Google Scholar 

  20. A. Mange P. Couble J.-C. Prudhomme (1996) ArticleTitleTwo alternative promoters drive the expression of the cytoplasmic actin A4 gene of Bombyx mori. Gene 183 191–199 Occurrence Handle10.1016/S0378-1119(96)00558-6 Occurrence Handle1:CAS:528:DyaK28XntlOhtbY%3D Occurrence Handle8996106

    Article  CAS  PubMed  Google Scholar 

  21. L.M. Mir M.F. Bureau J. Gehl R. Rangara D. Rouy J.M. Caillaud P. Delaere D. Dranellec B. Schwartz D. Scherman (1999) ArticleTitleHigh-efficiency gene transfer into skeletal muscle mediated by electric pulses. Pro Natl Acad Sci USA 96 4262–4267 Occurrence Handle10.1073/pnas.96.8.4262 Occurrence Handle1:CAS:528:DyaK1MXjs1ymur8%3D

    Article  CAS  Google Scholar 

  22. F. Muller D.W. Williams J. Kobolak L. Gauvry G. Goldspink L. Orban N. Maclean (1997) ArticleTitleActivator effect of coinjected enhancers on the muscle-specific expression of promoters in zebrafish embryos. Mol Reprod Dev 47 404–412 Occurrence Handle10.1002/(SICI)1098-2795(199708)47:4<404::AID-MRD6>3.3.CO;2-F Occurrence Handle1:CAS:528:DyaK2sXkt1CjsLk%3D Occurrence Handle9211424

    Article  CAS  PubMed  Google Scholar 

  23. Y.K. Nam M.S. Kim H.H. Lee D.S. Kim (1996) ArticleTitleProduction of transgenic homozygous diploid in mud loach (Misgurnus mizolepis), I: transfer of luciferase gene and evaluation of mud loach expression vector. J Acquacult 9 293–300

    Google Scholar 

  24. Y.K. Nam C.H. Noh D.S. Kim (1999) ArticleTitleTransmission and expression of an integrated reporter construct up to three generations in transgenic mud loach. Misgurnus mizolepis. Aquacult 172 229–245 Occurrence Handle10.1016/S0044-8486(98)00433-5 Occurrence Handle1:CAS:528:DyaK1MXpslWisA%3D%3D

    Article  CAS  Google Scholar 

  25. Y.K. Nam J.K. Noh Y.S. Cho H.J. Cho K.-N. Cho C.G. Kim D.S. Kim (2001a) ArticleTitleDramatically accelerated growth and extraordinary gigantism of transgenic mud loach Misgurnus mizolepis. Transgen Res 10 353–362 Occurrence Handle1:CAS:528:DC%2BD3MXntlSisr4%3D

    CAS  Google Scholar 

  26. Y.K. Nam J.K. Noh Y.S. Cho H.J. Cho C.G Kim D.S. Kim (2001b) ArticleTitleAccelerated growth, gigantism and likely sterility in autotransgenic triploid mud loach Misgurnus mizolepis. J World Aquacult Soc 32 353–363

    Google Scholar 

  27. J.K. Noh K.N. Cho Y.K. Nam D.S. Kim C.G. Kim (1999) ArticleTitleGenomic organization and sequence of the mud loach (Misgurnus mizolepis) growth hormone gene: a comparative analysis of teleost growth hormone gene. Mol Cells 9 638–645 Occurrence Handle1:CAS:528:DC%2BD3cXltFCitQ%3D%3D Occurrence Handle10672931

    CAS  PubMed  Google Scholar 

  28. P.E. Olsson P. Kling L.J. Erkell P. Kille (1995) ArticleTitleStructural and functional analysis of the rainbow trout (Oncorhyncus mykiss) metallothionein-A gene. Eur J Biochem 230 344–349 Occurrence Handle1:CAS:528:DyaK2MXlvFaltb8%3D Occurrence Handle7601121

    CAS  PubMed  Google Scholar 

  29. J.E. Onyia D.L. Halladay J.L. Messina (1995) ArticleTitleOne of three CCArGG box/serum response elements of the β-actin gene is an insulin-responsive element. Endocrinology 136 306–15 Occurrence Handle1:CAS:528:DyaK2MXjt1Kntbw%3D Occurrence Handle7828546

    CAS  PubMed  Google Scholar 

  30. S. Orita K. Makino T. Kawamoto H. Niwa H. Sugiyama T. Kakunaga (1989) ArticleTitleIdentification of a site that mediates transcriptional response of the human β-actin gene to serum factors. Gene 30 13–19

    Google Scholar 

  31. J.H. Park J.J. Lee S. Yoon J.-S. Lee S.Y. Choe J. Choe E.-H. Park C.G. Kirn (2001) ArticleTitleGenomic cloning of the Hsc71 gene in the hermaphroditic teleost Rivulus marmoratus and analysis of its expression in skeletal muscle: identification of a novel muscle-preferred regulatory element. Nucleic Acids Res 29 3041–3050 Occurrence Handle10.1093/nar/29.14.3041 Occurrence Handle1:CAS:528:DC%2BD3MXlslKis70%3D Occurrence Handle11452029

    Article  CAS  PubMed  Google Scholar 

  32. A.T. Sands T.N. Hansen F.J. Demayo L.A. Stanley L. Xin R.J. Schwartz (1993) ArticleTitleCytoplasmic β-actin promoter produces germ cell and preimplantation embryonic transgene expression. Mol Reprod Dev 34 117–126 Occurrence Handle1:CAS:528:DyaK3sXit1Kkurg%3D Occurrence Handle8442950

    CAS  PubMed  Google Scholar 

  33. F. Sanger S. Nicklen A.R. Coulson (1977) ArticleTitleDNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74 5463–5467 Occurrence Handle271968

    PubMed  Google Scholar 

  34. M.E. Schultz R.J. Schultz (1982) ArticleTitleInduction of hepatic tumors with 7,12-dimethylbenz[a]anthracene in two species of viviparous fishes (genus Poeciliopsis). Environ Res 27 337–351 Occurrence Handle1:CAS:528:DyaL38XhvFCqsr0%3D Occurrence Handle6806092

    CAS  PubMed  Google Scholar 

  35. B.T. Spear T. Longley S. Moulder S.L. Wang M.L. Peterson (1995) ArticleTitleA sensitive lacZ-based expression vector for analyzing transcriptional control elements in eukaryotic cells. DNA Cell Biol 14 635–642 Occurrence Handle1:CAS:528:DyaK2MXntFejtrw%3D Occurrence Handle7626223

    CAS  PubMed  Google Scholar 

  36. S. Takagi T. Sasado G. Tamiya K. Ozato Y. Wakamatsu A. Takeshita M. Kimura (1994) ArticleTitleAn efficient expression vector for transgenic medaka construction. Mol Mar Biol Biotechnol 3 192–199 Occurrence Handle1:CAS:528:DyaK2MXit1yrsrY%3D Occurrence Handle8000477

    CAS  PubMed  Google Scholar 

  37. B. Venkatesh B.H. Tay G. Elgar S. Brenner (1996) ArticleTitleIsolation, characterization and evolution of nine pufferfish (Fugu rubripes) actin genes. J Mol Biol 259 655–665 Occurrence Handle10.1006/jmbi.1996.0347 Occurrence Handle1:CAS:528:DyaK28XjvFajs7g%3D Occurrence Handle8683572

    Article  CAS  PubMed  Google Scholar 

  38. M.C. Walters S. Fiering J. Eidemiller W. Magis M. Groudine D.I. Martin (1995) ArticleTitleEnhancers increase the probability but not the level of gene expression. Proc Natl Acad Sci USA 92 7125–7129 Occurrence Handle1:CAS:528:DyaK2MXntVSkurk%3D Occurrence Handle7624382

    CAS  PubMed  Google Scholar 

  39. M. Zafarullah K. Bonham L. Gedamu (1988) ArticleTitleStructure of the rainbow trout metallothionein B gene and characterization of its metal-responsive region. Mol Cell Biol 8 4469–4476 Occurrence Handle1:CAS:528:DyaL1MXit1Kgsrc%3D Occurrence Handle3185557

    CAS  PubMed  Google Scholar 

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Acknowledgements

We thank Dr. Eun-Ho Park for his encouragement and valuable discussions, and Dr. Tim D. Williams at the University of Birmingham in England for the critical comments on the manuscript. This work was partially supported by Hanyang University Faculty Research grants to C.G.K. and the Brain Korea 21 projects to C.G.K. and D.S.K.

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Authors and Affiliations

  1. Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 133-791, South Korea

    Jae Koo Noh, Kyu-Nam Cho, Eun Hwa Han, AeRi Kim & Chul Geun Kim

  2. Department of Environmental Science, Graduate School, Hanyang University, Seoul 133-791, South Korea

    Jae-Seong Lee

  3. Department of Aquaculture, College of Fisheries Sciences, Pukyong National University, Pusan 608-737, South Korea

    Dong Soo Kim

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  1. Jae Koo Noh
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  2. Kyu-Nam Cho
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Correspondence to Chul Geun Kim.

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Noh, J., Cho, KN., Han, E. et al. Genomic Cloning of Mud Loach Misgurnus mizolepis (Cypriniformes, Cobitidae) β-Actin Gene and Usefulness of Its Promoter Region for Fish Transgenesis . Mar. Biotechnol. 5, 244–252 (2003). https://doi.org/10.1007/s10126-002-0066-1

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