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Concentration of Marine Birnavirus from Seawater with a Glass Fiber Filter Precoated with Bovine Serum Albumin

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Abstract

In the present study an efficient method for sampling the marine birnavirus (MABV) gene from seawater was developed. MABV gene was monitored by a specific polymerase chain reaction. When Millipore filters were used, MABV was efficiently collected on a filter with 0.05-µm pore size. When both millipore and glass fiber filters were used, MABV was recovered from both filters. Use of plain glass fiber filters resulted in poor recovering efficiency. However, coating the glass fiber filters with 1% bovine serum albumin trapped MABV efficiently. Combining concentration on glass fiber filters with polymerase chain reaction is quantitative, economic and fast, suggesting that this method can be used to detect genetically identified fish disease viruses, algal viruses, and phages.

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References

  1. InstitutionalAuthorNameAmerican Public Health Association. (1998) Virus concentration from small sample volumes by adsorption to and elution from microporous filters. Standard methods for the examination of water and wastewater. American Public Health Association New York, N. Y. 9:117–9:119

    Google Scholar 

  2. S.L. Blake W.B. Schill P.E. Mcallister M. Lee J.T. Singer B.L. Nicholson (1995) ArticleTitleDetection and identification of aquatic birnaviruses by PCR assay. J Clin Microbiol 33 835–839 Occurrence Handle1:CAS:528:DyaK2MXlsVOms7o%3D Occurrence Handle7790447

    CAS  PubMed  Google Scholar 

  3. J.L. Fryer A. Yusha K.S. Pilcher (1965) ArticleTitleThe in vitro cultivation of tissue and cells of Pacific salmon and steelhead trout. Ann N Y Acad Sci 126 555–586 Occurrence Handle5220177

    PubMed  Google Scholar 

  4. C. Gantzer S. Senouci A. Maul Y. Levi L. Schwatzbrod (1997) ArticleTitleEnterovirus genomes in wastewater: concentration on glass wool and glass powder and detection by RT-PCR. J Virol Methods 65 265–271 Occurrence Handle10.1016/S0166-0934(97)02193-9 Occurrence Handle1:CAS:528:DyaK2sXktVSksbs%3D Occurrence Handle9186950

    Article  CAS  PubMed  Google Scholar 

  5. N. Hosono S. Suzuki R. Kusuda (1996) ArticleTitleGenogrouping of birnaviruses isolated from marine fish: a comparison of VP2/NS junction regions on genome segment A. J Fish Dis 19 295–302 Occurrence Handle1:CAS:528:DyaK28Xltl2nu7k%3D

    CAS  Google Scholar 

  6. N. Jothikumar P. Khanna R. Paulmurugan S. Kamatchiammal P. Padmanabhan (1995) ArticleTitleA simple device for the concentration and detection of enterovirus, hepatitis E virus and rotavirus from water samples by reverse transcriotion-polymerase chain reaction. J Virol Methods 55 401–415 Occurrence Handle10.1016/0166-0934(95)00089-9 Occurrence Handle1:CAS:528:DyaK2MXps1Gitbc%3D Occurrence Handle8609205

    Article  CAS  PubMed  Google Scholar 

  7. S. Kitamura S. Suzuki (2000) ArticleTitleOccurrence of marine birnavirus through the year in coastal seawater in the Uwa sea. Mar Biotechnol 2 188–194 Occurrence Handle1:CAS:528:DC%2BD3cXjtF2jt74%3D Occurrence Handle10811959

    CAS  PubMed  Google Scholar 

  8. R. Kusuda A. Kawarasaki (1993) ArticleTitleEstablishment and characterization of a cell line derived from the kidney of red sea bream, Pagrus major. Suisanzoshoku 41 455–460

    Google Scholar 

  9. R. Kusuda Y. Nishi N. Hosono S. Suzuki (1993) ArticleTitleSerological comparison of birnaviruses isolated from several species of marine fish in south west Japan. Fish Pathol 28 91–92

    Google Scholar 

  10. D. Leon R.C. Shieh R.S. Baric M.D. Sobsey (1990) Detection of enteroviruses and hepatitis A virus in environmental samples by gene probes and polymerase chain reaction. Advances in Water Analysis and Treatment: Proceeding of the Water Quality Technology Conference. American Water Works Association Denver, Colo. 833–853

    Google Scholar 

  11. S. Maheshkumar S.M. Goyal R.B. Peterson P.P. Economon (1991) ArticleTitleMethod for the concentration of infectious pancreatic necrosis virus from hatchery water. J Virol Methods 31 211–218 Occurrence Handle10.1016/0166-0934(91)90159-W Occurrence Handle1:STN:280:By6A38vjvFA%3D Occurrence Handle1864906

    Article  CAS  PubMed  Google Scholar 

  12. P.E. McAllister J. Bebak (1997) ArticleTitleInfectious pancreatic necrosis virus in environment: relationship to effluent from aquaculture facilities. J Fish Dis 20 201–207

    Google Scholar 

  13. K. Nakajima Y. Maeno M. Aritomo K. Inoue M. Sorimachi (1993) ArticleTitleViral deformity of yellowtail fingerings. Gyobo Kenkyu 28 125–129

    Google Scholar 

  14. K.J. Schwab R.D. Leon R.S. Baric M.D. Sobsey (1991) Detection of rotaviruses, enteroviruses and hepatitis A virus by reverse-transcriptase polymerase chain reaction. Advances in Water Analysis and Treatment: Proceeding of the Water Quality Technology Conference. American Water Works Association Denver, Colo. 475–491

    Google Scholar 

  15. S. Suzuki T. Nakata M. Kamakura M. Yoshinaka Y. Furukawa Y. Yamashita R. Kusuda (1997a) ArticleTitleIsolation of birnavirus from Agemaki (jack knife clam) Shinonovacura consticta and survey of virus using PCR technique. Fisheries Sci 63 563–566 Occurrence Handle1:CAS:528:DyaK2sXls1yms7o%3D

    CAS  Google Scholar 

  16. S. Suzuki N. Hosono R. Kusuda (1997b) ArticleTitleDetection of aquatic birnavirus gene from marine fish using a combination of reverse transcription and nested PCR. J Mar Biotechnol 5 205–209 Occurrence Handle1:CAS:528:DyaK2sXmtVGlsr4%3D

    CAS  Google Scholar 

  17. S. Suzuki M. Kamakura R. Kusuda (1998) ArticleTitleIsolation of birnavirus from Japanese peal oyster Pinctada fucata. Fisheries Sci 64 342–343 Occurrence Handle1:CAS:528:DyaK1cXivFCqsLo%3D

    CAS  Google Scholar 

  18. S. Suzuki S. Kitamura H.X. Chiura (2001) ArticleTitleAquabirnavirus widely distributes in the ocean, providing a dissolved RNA pool. Microb Environ 16 191–196 Occurrence Handle10.1264/jsme2.2001.191

    Article  Google Scholar 

  19. Y. Tsai M.D. Sobsey L.R. Sangermano C.J. Palmer (1993) ArticleTitleSimple method of concentrating enteroviruses and hepatitis A virus from seawage and ocean water for rapid detection by reverse transcriptase–polymerase chain reaction. Appl Microbiol 59 3488–3491 Occurrence Handle1:CAS:528:DyaK2cXhs1Sk

    CAS  Google Scholar 

  20. R.A. Watanabe J.L. Fryer J.S. Rohovec (1998) ArticleTitleMolecular filtration for recovery of waterborne viruses of fish. Appl Environ Microbiol 54 1606–1609

    Google Scholar 

  21. K. Williams S. Blake A. Sweeney J.T. Singer B.L. Nicholson (1999) ArticleTitleMultiplex reverse transcriptase PCR assay for simultaneous detection of three fish viruses. J Clin Microbiol 37 4139–4141 Occurrence Handle1:CAS:528:DyaK1MXotV2ms78%3D Occurrence Handle10565946

    CAS  PubMed  Google Scholar 

  22. K. Wolf M.C. Quimby (1973) ArticleTitleFish viruses: buffers and methods for plaquing eight agents under normal atmosphere. Appl Microbiol 25 659–664 Occurrence Handle1:STN:280:CSyC28zhsVA%3D Occurrence Handle4349252

    CAS  PubMed  Google Scholar 

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Acknowledgements

We thank Shin-Ichi Nakano and Shin-Ichi Kitamura for their suggestions on this study. We also are grateful to John Bower for his critical review of this manuscript. This study was partly supported by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan.

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  1. Center for Marine Environmental Studies (CMES), Ehime University, Matsuyama 790-8577, Japan

    Shin-Ichiro Kamata & Satoru Suzuki

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  1. Shin-Ichiro Kamata
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  2. Satoru Suzuki
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Correspondence to Satoru Suzuki.

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Kamata, SI., Suzuki, S. Concentration of Marine Birnavirus from Seawater with a Glass Fiber Filter Precoated with Bovine Serum Albumin . Mar. Biotechnol. 5, 157–162 (2003). https://doi.org/10.1007/s10126-002-0057-2

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