Abstract
Pigments are coloring agents used widely in different industrial sectors. There is a demand for using natural pigments rather than synthetic dyes because of the health hazards caused by synthetic dyes. Many natural pigments have different medicinal activities which can contribute to the nutritional value of the product. This study was carried forward with marine yeasts which can produce pigments. A total of 4 marine yeast isolates were recovered from the mangrove area of Sundarbans, West Bengal, India. Among them, the isolate KSB1 produced 856 µg/g total concentration of carotenoid pigment and the dry mass weight was 3.56 g/L. The stability of the extracted pigments was checked using temperature, pH, UV light exposure time, and different saline conditions. The pigments were characterized using HPLC and FTIR analysis. All of the extracted pigments showed good antioxidant activity in DPPH, metal chelating, and reducing power assay. The pigments were also found to have good antibacterial activity against the bacterial pathogens Staphylococcus aureus, Listeria monocytogenes, and Escherichia coli. Carotenoid pigment from KSB1 was found to have maximum activity in all the pathogens. The cytogenotoxicity using onion roots and phytotoxicity analysis indicated that the pigments were non-toxic and safe for cells. Finally, the potential marine yeast was identified using 18 s rRNA sequencing and identified as Rhodotorula sp. KSB1 (Accession no. MH782232).
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References
Aamir S, Sutar S, Singh SK, Baghela A (2015) A rapid and efficient method of fungal genomic DNA extraction, suitable for PCR based molecular methods. Plant Pathol Quar 5(2):74–81
Aksu Z, Eren AT (2005) Carotenoids production by the yeast Rhodotorula mucilaginosa: use of agricultural wastes as a carbon source. Process Biochem 40(9):2985–2991. https://doi.org/10.1016/j.procbio.2005.01.011
Asema SU, Tanveer ST, Sultan S (2015) Analysis of soil samples for its physico-chemical parameters from Aurangabad City. Int J Innov Res Dev. 4(13).
Ashenafi EL, Nyman MC, Shelley JT, Mattson NS (2023) Spectral properties and stability of selected carotenoid and chlorophyll compounds in different solvent systems. Food Chem Adv 2:100178. https://doi.org/10.1016/j.focha.2022.100178
Atencio S, Verkempinck SH, Reineke K, Hendrickx M, Van Loey A (2022) Heat and light stability of pumpkin-based carotenoids in a photosensitive food: a carotenoid-coloured beverage. Foods 11(3):485. https://doi.org/10.3390/foods11030485
BaN N (2018) Lv P, Zhao WE (2018) Suppressive effects of lycopene and β-carotene on the viability of the human esophageal squamous carcinoma cell line EC109. Oncol Lett 15(5):6727–6732. https://doi.org/10.3892/ol.2018.8175
Banerjee S, Pandey K, Mazumdar S, Sarkar A, Rao KVB (2019) Characterization and applications of protease inhibitor compound from marine yeast Candida orthopsilosis KSSB3. Res J Biotechnol 14:43–52
Barlett M (2000) Diagnostic bacteriology, a study guide. F. A. Danis Co., Philadelphia, PA
Bartholomew JW, Mittwer T (1952) The gram stain. Bacteriol Rev 16(1):1–29
Bauer AW (1966) Antibiotic susceptibility testing by a standardised single disc method. Am J Clin Pathol 45:149–158
Caritá R, Marin-Morales MA (2008) Induction of chromosome aberrations in the Allium cepa test system caused by the exposure of seeds to industrial effluents contaminated with azo dyes. Chemosphere 72(5):722–725. https://doi.org/10.1016/j.chemosphere.2008.03.056
Carreira A, Ferreira LM, Loureiro V (2001) Production of brown tyrosine pigments by the yeast Yarrowia lipolytica. J Appl Microbiol 90(3):372–379. https://doi.org/10.1046/j.1365-2672.2001.01256.x
Chandra RK (1997) (1997) Food hypersensitivity and allergic disease: a selective review. American J Clin Nutr 66(2):526S-529S
Chen YS, Yanagida F, Chen LY (2009) Isolation of marine yeasts from coastal waters of northeastern Taiwan. Aquat Biol 8(1):55–60. https://doi.org/10.3354/ab00207
Chi ZM, Liu G, Zhao S, Li J, Peng Y (2010) Marine yeasts as biocontrol agents and producers of bio-products. Appl Microbiol Biotechnol 86:1227–1241. https://doi.org/10.1007/s00253-010-2483-9
Ciaraldi TP, Boeder SC, Mudaliar SR, Giovannetti ER, Henry RR, Pettus JH (2023) Astaxanthin, a natural antioxidant, lowers cholesterol and markers of cardiovascular risk in individuals with prediabetes and dyslipidaemia. Diabetes Obes Metab 25(7):1985–1994. https://doi.org/10.1111/dom.15070
Clarke H, Cowan ST (1952) Biochemical methods for bacteriology. J Gen Microbiol 6:187–197
Cong L, Chi Z, Li J, Wang X (2007) Enhanced carotenoid production by a mutant of the marine yeast Rhodotorula sp. hidai. J Ocean Univ China 6:66–71
Cook SM, Linden DR (1997) Use of rhodamine WT to facilitate dilution and analysis of atrazine samples in short-term transport studies. J Environ Qual 26(5):1438–1440. https://doi.org/10.2134/jeq1997.00472425002600050035x
Correa-Llantén DN, Amenábar MJ, Blamey JM (2012) Antioxidant capacity of novel pigments from an Antarctic bacterium. J Microbiol 50(3):374–379. https://doi.org/10.1007/s12275-012-2029-1
Davis AR, Collins J, Fish WW, Tadmor Y, Webber Iii CL, Perkins-Veazie P (2007) Rapid method for total carotenoid detection in canary yellow-fleshed watermelon. J Food Sci 72(5):S319-323. https://doi.org/10.1111/j.1750-3841.2007.00381.x
Dhaliwal MK, Chandra N (2016) Isolation of carotenoids producing marine red yeasts. Indian J Geo-Mar Sci 45(8):1029–1034
Dinis TC, Madeira VM, Almeida LM (1994) Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 315(1):161–169. https://doi.org/10.1006/abbi.1994.1485
Doyle WM (1992) Principles and applications of Fourier transform infrared (FTIR) process analysis. Process Control Qual 2(1):11–41.
El-Naggar NE, El-Ewasy SM (2017) Bioproduction, characterization, anticancer and antioxidant activities of extracellular melanin pigment produced by newly isolated microbial cell factories Streptomyces glaucescens NEAE-H. Sci Rep 7(1):42129. https://doi.org/10.1038/srep42129
El-Ziney MG, Abu Zaid EA, El-Naggar MY (2018) Characterization of carotenogenic Rhodotorula strains isolated from Delta Region, Egypt and their potential for carotenoids production. J Pure Appl Microbiol 12(2):587–599
Erbaş E, Üstündağ H, Öztürk E, Parlak SN, Atcalı T (2024) Astaxanthin treatment reduces kidney damage and facilitates antioxidant recovery in lithium-intoxicated rats. Toxicon 241:107664. https://doi.org/10.1016/j.toxicon.2024.107664
Fell JW, Ahearn DG, Meyers SP, Roth FJ Jr (1960) Isolation of yeasts from Biscayne Bay, Florida and adjacent benthic areas 1, 2. Limnol Oceanogr 5(4):366–371. https://doi.org/10.4319/lo.1960.5.4.0366
Fischer ER, Hansen BT, Nair V, Hoyt FH, Dorward DW (2012) Scanning Electron Microscopy. Curr Protoc Microbiol 25:2B
Fukumoto LR, Mazza G (2000) Assessing antioxidant and prooxidant activities of phenolic compounds. J Agric Food Chem 48(8):3597–3604. https://doi.org/10.1021/jf000220w
Gerster H (1997) The potential role of lycopene for human health. J Am Coll Nutr 16:109–126. https://doi.org/10.1080/07315724.1997.10718661
Guo XY, Wang J, Wang NL, Kitanaka S, Yao XS (2007) 9, 10-Dihydrophenanthrene derivatives from Pholidota yunnanensis and scavenging activity on DPPH free radical. J Asian Nat Prod Res 9(2):165–174. https://doi.org/10.1080/10286020500480522
Hegazy GE, Abu-Serie MM, Abo-Elela GM, Ghozlan H, Sabry SA, Soliman NA, Abdel-Fattah YR (2020) In vitro dual (anticancer and antiviral) activity of the carotenoids produced by haloalkaliphilic archaeon Natrialba sp. M6. Sci Rep 10:5986. https://doi.org/10.1038/s41598-020-62663-y
Iatrou M, Papadopoulos A, Papadopoulos F, Dichala O, Psoma P (2014) Bountla A (2014) Determination of soil available phosphorus using the Olsen and Mehlich 3 methods for Greek soils having variable amounts of calcium carbonate. Commun Soil Sci Plant Anal 45(16):2207–2214. https://doi.org/10.1080/00103624.2014.911304
Jayanthi P, Lalitha P (2011) Reducing power of the solvent extracts of Eichhornia crassipes (Mart.) Solms. Int J Pharm Sci 3(3):126–128.
Johnson EA, An GH (1991) Astaxanthin from microbial sources. Crit Rev Biotechnol 11(4):297–326. https://doi.org/10.3109/07388559109040622
Joshi VK, Attri D, Bala A, Bhushan S (2003) Microbial pigments. Indian. J Biotechnol 2:362–369
Kato S, Takaichi S, Ishikawa T, Asahina M, Takahashi S, Shinomura T (2016) Identification and functional analysis of the geranylgeranyl pyrophosphate synthase gene (crtE) and phytoene synthase gene (crtB) for carotenoid biosynthesis in Euglena gracilis. BMC Plant Biol 16:1–2. https://doi.org/10.1186/s12870-015-0698-8
Kim JK, Kim JI, Lee NK, Hahm YT, Baik MY, Kim BY (2010) Extraction of β-carotene produced from yeast Rhodosporidium sp. and its heat stability. Food Sci Biotechnol 19:263–266. https://doi.org/10.1007/s10068-010-0038-6
Kohlmeier L, Kark JD, Gomez GE, Martin BC, Steck SE, Kardinaal AFM, Ringstad J, Thamm TM, Masaev V, Riemersma R, Moreno-Martin JM, Huttunene JK, Kok FJ (1997) Lycopene and myocardial infarction risk in the EURAMIC study. Am J Epidemiol 146:618–626. https://doi.org/10.1093/oxfordjournals.aje.a009327
Kohlmeyer J, Kohlmeyer E (2013) Marine mycology: the higher fungi. Elsevier
Kumar SRS, Rao KVB (2016) Postprandial anti-hyperglycemic activity of marine Streptomyces coelicoflavus SRBVIT13 mediated gold nanoparticles in streptozotocin induced diabetic male albino Wister rats. IET Nanobiotechnol 10(5):308–314. https://doi.org/10.1049/iet-nbt.2015.0094
Kutty SN, Philip R (2008) Marine yeasts—a review. Yeast 25:465–483. https://doi.org/10.1002/yea.1599
Lau W, Carvajal-Zarrabal O, Nolasco-Hipólito C, Kohei M, Gregory ZA, Abdullah MO, Toh S, Lihan S (2018) Production of pigments by Rhodotorula mucilaginosa. Malaysian J Microbiol 14(4):344–350
Lazarus CR, Koburger JA (1974) Identification of yeasts from the Suwannee River Florida estuary. Appl Microbiol 27(6):1108–1111. https://doi.org/10.1128/am.27.6.1108-1111.1974
Le Tutour B, Benslimane F, Gouleau MP, Gouygou JP, Saadan B, Quemeneur F (1998) Antioxidant and pro-oxidant activities of the brown algae, Laminaria digitata, Himanthalia elongata, Fucus vesiculosus, Fucus serratus and Ascophyllum nodosum. J Appl Phycol 10:121–129. https://doi.org/10.1023/A:1008007313731
Lee J, Heo SC, Kim Y (2024) Combination of oxaliplatin and β-carotene suppresses colorectal cancer by regulating cell cycle, apoptosis, and cancer stemness in vitro. Nutr Res Prac 18(1):62. https://doi.org/10.4162/nrp.2024.18.1.62
Li F, Xue F, Yu X (2017) GC–MS, FTIR and Raman analysis of antioxidant components of red pigments from Stemphylium lycopersici. Curr Microbiol 74(4):532–539. https://doi.org/10.1007/s00284-017-1220-3
Libkind D, van Broock M (2006) Biomass and carotenoid pigment production by patagonian native yeasts. World J Microbiol Biotechnol 22:687–692. https://doi.org/10.1007/s11274-005-9091-3
Lobo V, Patil A, Phatak A, Chandra N (2010) Free radicals, antioxidants and functional foods: impact on human health. Pharmacogn Rev 4(8):118. https://doi.org/10.4103/0973-7847.70902
Malik K, Tokkas J, Goyal S (2012) Microbial pigments: a review. Int J Microbial Res Technol 1(4):361–365
Mata-Gómez LC, Montañez JC, Méndez-Zavala A, Aguilar CN (2014) Biotechnological production of carotenoids by yeasts: an overview. Microb Cell Factories 13(1):1–11. https://doi.org/10.1186/1475-2859-13-12
Medentsev AG, Akimenko VK (1992) Mechanism of phytotoxic action of naphthoquinone pigments of the fungus Fusarium decemcellulare. Phytochem 31(1):77–79. https://doi.org/10.1016/0031-9422(91)83009-A
Meléndez-Martínez AJ, Vicario IM, Heredia FJ (2004) Stability of carotenoid pigments in foods. Arch Latinoam Nutr 54(2):209–215
Muthezhilan R, Ragul R, Pushpam AC, Narayanan RL, Hussain AJ (2014) Isolation, optimization and extraction of microbial pigments from marine yeast Rhodotorula Sp. (Amby109) as food colourants. Biosci Biotechnol Res Asia 11:271–278
Naghavi FS, Hanachi P, Soudi MR, Saboora A (2013) The capability of Rhodotorula slooffiae to Produce carotenoid. Zahedan J Res Med Sci 17(2):52–56
Obe G, Pfeiffer P, Savage JR, Johannes C, Goedecke W, Jeppesen P, Natarajan AT, Martınez-López W, Folle GA, Drets ME (2002) Chromosomal aberrations: formation, identification and distribution. Mut Res/fundam Mol Mech Mutagen 504(1–2):17–36. https://doi.org/10.1016/S0027-5107(02)00076-3
Pandey K, Saha P, Rao KB (2019) A study on the utility of immobilized cells of indigenous bacteria for biodegradation of reactive azo dyes. Prep Biochem Biotechnol 50(4):317–329. https://doi.org/10.1080/10826068.2019.1692219
Rani MH, Ramesh T, Subramanian J, Kalaiselvam M (2013) Production and characterization of melanin pigment from halophilic black yeast Hortaea werneckii. Int J Pharma Res Rev 2(8):9–17
Ranković BR, Kosanić MM, Stanojković TP (2011) Antioxidant, antimicrobial and anticancer activity of the lichens Cladonia furcata, Lecanora atra and Lecanora muralis. BMC Complement Altern Med 11(1):1–8. https://doi.org/10.1186/1472-6882-11-97
Rao P, Sudershan RV (2008) Risk assessment of synthetic food colours: a case study in Hyderabad, India. Int J Food Saf Nutr Public Health 1(1):68–87. https://doi.org/10.1504/IJFSNPH.2008.018857
Rathod JP, Vira C, Lali AM, Prakash G (2020) Metabolic engineering of Chlamydomonas reinhardtii for enhanced β-carotene and lutein production. Appl Biochem Biotechnol 190:1457–1469. https://doi.org/10.1007/s12010-019-03194-9
Razzak SA (2024) Comprehensive overview of microalgae-derived carotenoids and their applications in diverse industries. Algal Res 7:103422. https://doi.org/10.1016/j.algal.2024.103422
Rodrigues TV, Amore TD, Teixeira EC, de Medeiros Burkert JF (2019) Carotenoid production by Rhodotorula mucilaginosa in batch and fed-batch fermentation using agroindustrial byproducts. Food Technol Biotechnol 57(3):388. https://doi.org/10.17113/ftb.57.03.19.6068
Rodriguez-Amaya DB (2010) (2010) Quantitative analysis, in vitro assessment of bioavailability and antioxidant activity of food carotenoids—a review. J Food Compos Anal 23(7):726–740. https://doi.org/10.1016/j.jfca.2010.03.008
Rodriguez-Carmona M, Kvansakul J, Alister Harlow J, Kopcke W, Schalch W, Barbur JL (2006) The effects of supplementation with lutein and/or zeaxanthin on human macular pigment density and colour vision. Ophthalmic Physiol Opt 26:137–147. https://doi.org/10.1111/j.1475-1313.2006.00386.x
Saha P, Rao KV (2020) Biotransformation of Reactive Orange 16 by alkaliphilic bacterium Bacillus flexus VITSP6 and toxicity assessment of biotransformed metabolites. Int J Environ Sci Technol 17:99–114. https://doi.org/10.1007/s13762-019-02256-z
Saha N, Samanta AK, Chaudhuri S, Dutta D (2015) Characterization and antioxidant potential of a carotenoid from a newly isolated yeast. Food Sci Biotechnol 24:117–124. https://doi.org/10.1007/s10068-015-0017-z
Salama HM, Al Watban AA, Al-Fughom AT (2011) Effect of ultraviolet radiation on chlorophyll, carotenoid, protein and proline contents of some annual desert plants. Saudi J Biol Sci 18(1):79–86. https://doi.org/10.1016/j.sjbs.2010.10.002
Sankari M, Hridya H, Sneha P, Doss CG, Ramamoorthy S (2017) Effect of UV radiation and its implications on carotenoid pathway in Bixa orellana L. J Photochem Photobiol B Biol 176:136–144. https://doi.org/10.1016/j.jphotobiol.2017.10.002
Sarkar A, Rao KVB (2016) Marine yeast: a potential candidate for biotechnological applications—a review. Asian J Microbiol Biotechnol Environ Sci 18:627–634
Sarkar A, Rao KVB (2023) Unraveling anticancer potential of a novel serine protease inhibitor from marine yeast Candida parapsilosis ABS1 against colorectal and breast cancer cells. World J Microbiol Biotechnol 39:225. https://doi.org/10.1007/s11274-023-03670-9
Sarkar S, Pramanik A, Mitra A, Mukherjee J (2010) Bioprocessing data for the production of marine enzymes. Mar Drugs 8(4):1323–1372. https://doi.org/10.3390/md8041323
Sarkar A, Philip AM, Thakkar DP, Wagh MS, Rao KVB (2020) In vitro Antioxidant activity of extracellular l-glutaminase enzyme isolated from marine yeast Rhodotorula sp. DAMB1. Res J Pharm Technol 13:209–215. https://doi.org/10.5958/0974-360X.2020.00042.6
Sarkar A, Ghosh D, Das S, Rao KVB (2021) Antioxidant and antibacterial activity of biogenic zirconium oxide nanoparticles from Candida orthopsilosis DSB1 isolated from backwaters of Sunderbans, West Bengal. Int J Nanopart 13:174–194. https://doi.org/10.1504/IJNP.2021.118111
Scarpi C, Ninci F, Centini M, Anselmi C (1998) High-performance liquid chromatography determination of direct and temporary dyes in natural hair colourings. J Chromatogr A 796(2):319–325
Simova ED, Frengova GI, Beshkova DM (2004) Synthesis of carotenoids by Rhodotorula rubra GED8 co-cultured with yogurt starter cultures in whey ultrafiltrate. J Ind Microbiol Biotechnol 31(3):115–121. https://doi.org/10.1007/s10295-004-0122-0
Singh S, Kumar P, Gopalan N, Shrivastava B, Kuhad RC, Chaudhary HS (2012) Isolation and partial characterization of actinomycetes with antimicrobial activity against multidrug resistant bacteria. Asian Pac J Trop Biomed 2(2):S1147-1150. https://doi.org/10.1016/S2221-1691(12)60375-X
Singhal M, Paul A, Singh HP (2014) Synthesis and reducing power assay of methyl semicarbazone derivatives. J Saudi Chem Soc 18(2):121–127. https://doi.org/10.1016/j.jscs.2011.06.004
Soare JR, Dinis TC, Cunha AP, Almeida L (1997) Antioxidant activities of some extracts of Thymus zygis. Free Rad Res 26(5):469–478. https://doi.org/10.3109/10715769709084484
Sokolowska-Gajda J, Freeman HS, Reife A (1996) Synthetic dyes based on environmental considerations Part 2: Iron complexes formazan dyes. Dyes pigments 30(1):1–20. https://doi.org/10.1016/0143-7208(95)00048-8
Srivastava R (2021) Physicochemical, antioxidant properties of carotenoids and its optoelectronic and interaction studies with chlorophyll pigments. Sci Rep 11(1):18365. https://doi.org/10.1038/s41598-021-97747-w
Stahl W, Sies H (2003) Antioxidant activity of carotenoids. Mol Asp Med 24(6):345–351. https://doi.org/10.1016/S0098-2997(03)00030-X
Tanngoen PI, Lamlertthon S, Tiyaboonchai W (2019) Effects of alpha-mangostin and citronella oil against acne-causing bacteria. Int J Pharm Sci 11:45–49
Tuli HS, Chaudhary P, Beniwal V, Sharma AK (2015) Microbial pigments as natural color sources: current trends and future perspectives. J Food Sci Technol 52:4669–4678. https://doi.org/10.1007/s13197-014-1601-6
Tünay O, Kabdasli I, Eremektar G, Orhon D (1996) Color removal from textile wastewaters. Water Sci Technol 34(11):9–16. https://doi.org/10.1016/S0273-1223(96)00815-3
Tuormaa TE (1994) The adverse effects of food additives on health: a review of the literature with a special emphasis on childhood hyperactivity. J Orthomol Med 9:225
Ushakumari UN, Ramanujan R (2013) Isolation of astaxanthin from marine yeast and study of its pharmacological activity. Int Curr Pharm J 2(3):67–69
Vendruscolo F, Tosin I, Giachini AJ, Schmidell W, Ninow JL (2014) Antimicrobial activity of Monascus pigments produced in submerged fermentation. J Food Process Preserv 38(4):1860–1865. https://doi.org/10.1111/jfpp.12157
Visalakchi S, Muthumary J (2009) Antimicrobial activity of the new endophytic Monodictys castaneae SVJM139 pigment and its optimization. African J Microbiol Res 2009 3(9):550–556
Wahyuningsih S, Wulandari L, Wartono MW, Munawaroh H, Ramelan AH (2017) The effect of pH and color stability of anthocyanin on food colorant. In IOP conference series: Materials science and engineering 193(1):012047 (IOP Publishing)
Wang L, Liu Z, Jiang H, Mao X (2021) Biotechnology advances in β-carotene production by microorganisms. Trends Food Sci 111:322–332. https://doi.org/10.1016/j.tifs.2021.02.077
Willey JM, Sherwood LM, Woolverton CJ (2008) Prescott, Harley, and Klein’s microbiology. McGraw-Hill
Wissgott U, Bortlik K (1996) Prospects for new natural food colorants. Trends Food Sci Technol 7(9):298–302. https://doi.org/10.1016/0924-2244(96)20007-X
Wong FC, Yong AL, Ting EP, Khoo SC, Ong HC, Chai TT (2014) Antioxidant, metal chelating, anti-glucosidase activities and phytochemical analysis of selected tropical medicinal plants. Iranian J Pharm Res 13(4):1409
Xie WJ, Liu M, Zhang X, Zhang YG, Jian ZH, Xiong XX (2024) Astaxanthin suppresses LPS-induced myocardial apoptosis by regulating PTP1B/JNK pathway in vitro. Int Immunopharmacol 127:111395. https://doi.org/10.1016/j.intimp.2023.111395
Yan X, Chuda Y, Suzuki M, Nagata T (1999) Fucoxanthin as the major antioxidant in Hijikia fusiformis, a common edible seaweed. Biosci Biotechnol Biochem 63(3):605–607. https://doi.org/10.1271/bbb.63.605
Zaky AS, Tucker GA, Daw ZY, Du C (2014) Marine yeast isolation nd industrial application. FEMS Yeast Res 14:813–825. https://doi.org/10.1111/1567-1364.12158
Zhao GR, Xiang ZJ, Ye TX, Yuan YJ, Guo ZX (2006) Antioxidant activities of Salvia miltiorrhiza and Panax notoginseng. Food Chem 99(4):767–774. https://doi.org/10.1016/j.foodchem.2005.09.002
Zhao Y, Guo L, Xia Y, Zhuang X, Chu W (2019) Isolation, identification of carotenoid-producing Rhodotorula sp. from marine environment and optimization for carotenoid production. Mar Drugs 17(3):161. https://doi.org/10.3390/md17030161
Zhenming C, Zhiqiang L, Lingmei G, Fang G, Chunling MA, Xianghong W, Haifeng LI (2006) Marine yeasts and their applications in mariculture. J Ocean Un China 5:251–256. https://doi.org/10.1007/s11802-006-0010-5
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Banerjee, S., Sarkar, A. & Rao, K.V.B. Extraction and characterization of carotenoid pigments with antioxidant and antibacterial potential from marine yeast Rhodotorula sp. KSB1. Int Microbiol (2024). https://doi.org/10.1007/s10123-024-00529-3
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DOI: https://doi.org/10.1007/s10123-024-00529-3