Abstract
The present study was conducted to study the composition of gut microbiome in the advanced fingerling and fingerling stage of striped pangasius catfish and catla during healthy and diseased conditions. Healthy pangasius and catla fishes were obtained from commercial farms and injected with the LD50 dose of A. hydrophila. The intestinal samples from the control and injected group were collected and pooled for 16 s metagenomic analysis. Community analysis was performed by targeting the 16 s rRNA gene to explore and compare the gut microbiota composition of these fishes. The operational taxonomic units (OTUs) consisted of four major phyla: Bacteroidia, Proteobacteria, Firmicutes, and Actinobacteria. Alpha and beta diversity indices were carried out to understand the diversity of microbes within and between a sample. While comparing the advanced fingerling and fingerling stage gut microbiome of Pangasius catfish, the dominance of Proteobacteria was found in fingerlings, whereas Firmicutes and Bacteroides were found in advanced fingerlings. In catla, Proteobacteria and Bacteroides were predominant. Taxonomic abundance of the microbiota in control and diseased Pangasius and catla fishes at phylum, class, order, family, genus, and species levels were also depicted. The present study is the first of its kind, and it will help to identify the diversity of novel potential bacterial species involved in disease protection of fishes. It can lead to the development of sustainable prophylactic measures against (re-)emerging bacterial diseases in aquaculture.
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Data availability
The data used in the present study are available in the NCBI database under the accession numbers PRJNA932971, PRJNA932969, and PRJNA932968.
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Acknowledgements
The authors sincerely thank Dr. C. N. Ravishankar, the Director and Vice-Chancellor of the Indian Council of Agricultural Research- Central Institute of Fisheries Education, Mumbai, India, for providing support and necessary facilities for this experiment.
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R. Bharathi Rathinam- Experimental trial, data curation and analysis, compilation and original draft preparation; Gayatri Tripathi- Conceptualization, overall supervision, resources and final correction of the manuscript; Basanta Kumar Das- Data analysis and reviewing of manuscript; Ratnesh Jain- Data interpretation and reviewing of manuscript; Arpit Acharya- Experimental trial and data analysis.
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Bharathi Rathinam, R., Tripathi, G., Das, B.K. et al. Comparative analysis of gut microbiome in Pangasionodon hypopthalmus and Labeo catla during health and disease. Int Microbiol (2024). https://doi.org/10.1007/s10123-024-00494-x
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DOI: https://doi.org/10.1007/s10123-024-00494-x