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Genomics analysis of three phosphorus-dissolving bacteria isolated from Torreya grandis soil

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Abstract

With the increasingly serious problem of phosphorus deficiency in the subtropical zone, chemical fertilizers are widely used. But it pollutes the environment. Phosphorus-solubilizing microorganisms (PSMs) are referred to as a new solution to this problem. We explored the phosphorus-dissolving characteristics of PSB strains isolated from the rhizosphere soil of Torreya grandis to provide a theoretical basis for selecting the strain for managing phosphorus deficiency in subtropical soils and also provides a more sufficient theoretical basis for the utilization of PSMs. From 84 strains, three strains exhibiting high phosphorus solubility and strong IAA producing capacity were selected through a series of experiments. The phosphate-solubilizing capacity of the three selected strains W1, W74, and W83 were 339.78 mg/L, 332.57 mg/L, and 358.61 mg/L, respectively. Furthermore, W1 showed the strongest IAA secreting capacity of 8.62 mg/L, followed by W74 (7.58 mg/L), and W83 (7.59 mg/L). Determination by metabolites, it was observed that these three strains dissolved phosphorus by secreting a large amount of lactic acid, aromatic acid, and succinic acid. The genome of these PSBs were sequenced and annotated in this study. Our results revealed that PSB primarily promotes their metabolic pathway, especially carbon metabolism, to secrete plenty organic acids for dissolving insoluble phosphorus.

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Data availability

The data presented in the study are deposited in the NCBI’s SRA repository, BioProject number PRJNA891368 (W1 genome), PRJNA891423 (W74 genome) and PRJNA891438 (W83 genome).

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Funding

This work was supported by the “Pioneer” and “Leading Goose” R&D Program of Zhejiang(2022C02009 and 2022C02061); the National Natural Science Foundation of China (Grant no. 32171830,); The Breeding of New Varieties of Torreya grandis Program (2021C02066-11); Scientific R&D Foundation for Talent Start-up Project of Zhejiang A&F University (2020FR073).

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  1. Qi Wang and Chenliang Yu contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou, China

    Qi Wang, Chenliang Yu, Congcong Kong, Hao Zeng, Weiwu Yu & Jiasheng Wu

  2. School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, China

    Qi Wang, Chenliang Yu, Congcong Kong, Hao Zeng, Weiwu Yu & Jiasheng Wu

  3. NFGA Engineering Research Center for Torreya Grandis ‘Merrillii’, Zhejiang A&F University, Hangzhou, 311300, China

    Weiwu Yu & Jiasheng Wu

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  1. Qi Wang
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Contributions

JiaSheng Wu and WeiWu Yu: provides Funding acquisition and Project administration. Chenliang Yu, Qi Wang, and WeiWu Yu: did formal analysis. Qi Wang: investigation, data curation. CongCong Kong and Hao Zeng: data curation and visualization. Qi Wang, Chenliang Yu, WeiWu Yu, and JiaSheng Wu: did the manuscript writing and review. All authors contributed to the article and approved the submitted manuscript.

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Correspondence to Weiwu Yu or Jiasheng Wu.

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Wang, Q., Yu, C., Kong, C. et al. Genomics analysis of three phosphorus-dissolving bacteria isolated from Torreya grandis soil. Int Microbiol 27, 361–376 (2024). https://doi.org/10.1007/s10123-023-00393-7

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