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Phosphate solubilization potential and stress tolerance of rhizobacteria from rice soil in Northern Thailand

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Abstract

Plant growth-promoting rhizobacteria (PGPR) are known to influence plant growth by various direct or indirect mechanisms. A total of 216 phosphate-solubilizing bacterial isolates were isolated from different rice rhizospheric soil in Northern Thailand. These isolate were screened in vitro for their plant growth-promoting activities such as solubilization of inorganic phosphate, ammonia (NH3), catalase and cell wall-degrading enzyme activity. It was found that 100% solubilized inorganic phosphate, 77.77% produced NH3 and most of the isolates were positive for catalase. In addition, some strains also produced cell wall-degrading enzymes such as protease (7%), chitinase (1%), cellulase (3%) and β-glucanase (3%), as evidenced by phenotypic biochemical test and quantitative assay using spectrophotometry. The isolates could exhibit more than two or three plant growth-promoting (PGP) traits, which may promote plant growth directly or indirectly or synergistically. Part of this study focused on the effect of NaCl, temperature, and pH on a specific the bacterial isolate Acinetobacter CR 1.8. Strain CR 1.8 was able to grow on up to 25% NaCl, between 25 and 55°C, and at pH 5–9. Maximum solubilization of tricalcium phosphate and aluminium phosphate was obtained at neutral pH, and 37°C. Strain CR 1.8 had protease activity but no cellulase, β-glucanase and cellulase activities.

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Abbreviations

c.f.u.:

Colony forming units

g :

Centrifugal field

A :

Absorbance (−log T)

PGP:

Plant growth-promoting

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Acknowledgement

A grant from The Commission of Higher Education is greatly appreciated. We thank Dr. Peter Green, NCIMB, UK for helping edit the manuscript.

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Authors and Affiliations

  1. Department of Biotechnology, Graduated School, Chiang Mai University, Chiang Mai, Thailand

    Mathurot Chaiharn

  2. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand

    Saisamorn Lumyong

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  1. Mathurot Chaiharn
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  2. Saisamorn Lumyong
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Correspondence to Saisamorn Lumyong.

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Chaiharn, M., Lumyong, S. Phosphate solubilization potential and stress tolerance of rhizobacteria from rice soil in Northern Thailand. World J Microbiol Biotechnol 25, 305–314 (2009). https://doi.org/10.1007/s11274-008-9892-2

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  • DOI: https://doi.org/10.1007/s11274-008-9892-2

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