Abstract
We evaluated the biocompatibility of a dimethylpolysiloxane-coated micro-device which had been designed for monitoring real-time bladder volume in previous studies. The extract assay with dimethylpolysiloxane which had been used for coating the micro-device to measure the bladder volume was performed as an in vitro cytotoxicity test. For in vivo biocompatibility testing, the inflammatory responses around the implantation site of the micro-device in subcutaneous tissue of rat were assessed by light microscope with H&E stain and fluorescence microscope with ED1 stain and von Willebrand factor stain. The averages of cell viability in dimethylpolysiloxane group were 84.6% and 82.3% at 24 h and 72 h incubation, respectively. The qualitative evaluations with light and fluorescence microscope revealed that the inflammatory changes peaked during 2 weeks but almost disappeared at 4 weeks after implantation of devices. The quantitative evaluations for granulation layer formation and neovascularization showed that the thickness of the layer in dimethylpolysiloxane group peaked during 2 weeks but it came to be stabilized at 4 weeks as thin as at 2 weeks in control group, and the frequency of neovascularization was higher in dimethylpolysiloxane group than in control group but it was not increased with time. The dimethylpolysiloxane-coated micro-device is thought be a reliable bio-medical device.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Kakaday, T., Plunkett, M., McInnes, S., Jimmy Li, J.S., Voelcker, N.H. and Craig, J.E., IFMBE Proc., 2009, 23: 198
Tan, R., McClure, T., Lin, C.K., Jea, D., Dabiri, F., Massey, T., Sarrafzadeh, M., Srivastava, M., Montemagno, C.D., Schulam, P. and Schmidt, J., Biomed. Microdevices, 2009, 11: 259
Lee, D.S., Kim, S.J., Sohn, D.W., Choi, B., Lee, M.K., Lee, S.J. and Kim, S.W., Kaohsiung J. Med. Sci., 2011, 27(4): 133
Provost, B. and Sawan, M., Med. Biol. Eng. Comput., 1997, 35(6): 691
Pizzoferrato, A., Ciapetti, G., Stea S., Cenni, E., Arciola, C.R., Granch, D. and Savarino, L., Clin. Mater., 1994, 15(3): 173
Bélanger, M.C., Marois, Y., Roy, R., Mehri, Y., Wagner, E., Zhang, Z., King, M.W., Yang, M., Hahn, C. and Guidoin, R., Artif. Organs, 2000, 24: 879
Sasoglu, F.M., Bohl, A.J. and Layton, B.E., J. Micromech. Microeng., 2007, 17: 623
Tang, L. and Eaton, J.W., Am. J. Clin. Pathol., 1995, 103(4): 466
Anderson, J.M., Cardiovasc. Pathol., 1993, 2(3): 33S
Dalu, A., Blaydes, B.S., Lomax, L.G. and Delclos, K.B., Biomaterials, 2000, 21(19): 1947
Keegan, A.D., In “Cytokine reference, IL-4”, ed. by Oppenheim, J.J. and Feldman, M., Academic Press, San Diego, 2000, p. 167
Johnston, R.B. Jr., N. Eng. J. Med., 1988, 318: 747
Kim, B.S., Oha, J.M., Kim, K.S., Seo, K.S., Cho, J.S., Khang, G., Lee, H.B., Park, K. and Kim, M.S., Biomaterials, 2009, 30(5): 902
Taglinger, K., Day, M.J. and Foster, A.P., J. Comp. Pathol., 2007, 137(4): 211
Cotran, R.S., Kumar, V. and Collins, T., in “Robbins pathologic basis of disease,” 6th ed., ed. by Albert, D.M., Anthony, D.C., Aster, J., et al., W.B. Saunders Company, Philadelphia, 1999, p. 108
Klinge, U., Klosterhalfen, B. and Muller, M., Eur. J. Surg., 1999, 165(7): 665
Author information
Authors and Affiliations
Corresponding author
Additional information
This work was financially supported in part by the development program for future fundamental technology of the Ministry of Education, Science and Technology (No. 2009-0082114), and in part by the Healthy Medical Treatment Research and Development Program of the Ministry of Health & Welfare (No. A090481).
Rights and permissions
About this article
Cite this article
Lee, D.S., Kim, S.J., Sohn, J.H. et al. Biocompatibility of a PDMS-coated micro-device: Bladder volume monitoring sensor. Chin J Polym Sci 30, 242–249 (2012). https://doi.org/10.1007/s10118-012-1119-1
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10118-012-1119-1